EUROLAB
iso-11731-2-rapid-detection-of-legionella-in-water-systems
Environmental Microbiology Testing ASTM D3974 Fungi and Bacteria Detection in Environmental AirASTM D5465 Heterotrophic Plate Count in WaterASTM D5466 Enumeration of Airborne Bacterial SporesASTM D6386 Environmental Swab Test for Biofilm MonitoringASTM D6536 Field Sampling for Microbiological Water QualityASTM D6595 Fungal Detection in Building Material SurfacesASTM D6973 Bioaerosol Collection and Analysis from Environmental AirASTM D6974 Bioburden Testing of Environmental Sampling DevicesEN 10088 Mold Detection on Walls and Surfaces in Damp EnvironmentsEN 12322 Listeria monocytogenes Detection on Environmental SurfacesEN 13098 Assessment of Workplace Bioaerosol ContaminationEN 13098 Microbial Air Monitoring in Indoor WorkplacesEN 14031 Environmental Surface Sampling Using Contact PlatesEN 16407 Environmental Air Sampling for Fungal SporesEN ISO 14644-1 Air Cleanliness Classification Based on Microbial ConcentrationEN ISO 14644-2 Monitoring of Airborne Particulates and MicrobesEN ISO 14698-1 Air and Surface Monitoring for MicroorganismsEN ISO 8692 Microbiological Impact of Wastewater on AlgaeEPA 10029 Detection of Pseudomonas spp. in Surface WaterEPA 10029 Pseudomonas aeruginosa Detection in Surface WatersEPA 1103.1 Enterococci Testing in Recreational WaterEPA 1600 Fecal Coliform Analysis in Surface Water SamplesEPA 1600 Thermotolerant Coliform Detection in WastewaterEPA 1601 Coliphage Analysis in Ambient WatersEPA 1601 Coliphage Detection in GroundwaterEPA 1601 Somatic Coliphage Analysis in Environmental WatersEPA 1602 Coliphage Detection Using Single Agar Layer (SAL) TechniqueEPA 1603 E. coli Enumeration in Environmental WatersEPA 1604 Coliform and E. coli Detection Using m-ColiBlue24EPA 1605 Aeromonas Detection in Drinking and Environmental WaterEPA 1606 Salmonella Detection in Environmental WaterEPA 1607 Shigella Detection in Recreational Water SourcesEPA 1608 Mycobacteria Detection in Environmental MatricesEPA 1609 Norovirus Detection Using RT-qPCR in Environmental WaterEPA 1611 Enterococci Detection by TaqMan® qPCR in WaterEPA 1613 Monitoring Pathogenic Bacteria in Urban StormwaterEPA 1615 F-RNA Bacteriophage Detection in GroundwaterEPA 1621 Adenovirus Detection in Sewage SludgeEPA 1622 Giardia Cyst Detection in Raw WaterEPA 1623 Cryptosporidium and Giardia Detection in Environmental WatersEPA 1680 Detection of Yeasts and Molds in WastewaterEPA 1681 Actinomycetes Enumeration in Soil and WaterEPA 1682 Detection of Mold Spores in SoilEPA 1690 Quantification of Total Coliforms in Marine WaterEPA 1697 Quantitative PCR for Bacteroides in Surface WaterEPA 180.1 Turbidity Testing in Environmental Water SourcesEPA 200.8 Bacterial Contamination in Groundwater SystemsEPA 9132 Nitrate-Reducing Bacteria Detection in Environmental WaterEPA 9215 Heterotrophic Plate Count Using SimPlate TechniqueISO 10705-1 Bacteriophage Detection in Sewage and SludgeISO 10705-2 Detection of F-specific RNA Bacteriophages in WaterISO 11133 Preparation of Culture Media for Microbiological TestingISO 11290-2 Detection of Listeria spp. in Environmental SamplesISO 11348 Bioluminescence Test for Environmental ToxicityISO 11731 Legionella Detection in Environmental Water SamplesISO 11732 Ammonia-Oxidizing Microorganisms in Natural WatersISO 14189 Detection of Sulfite-Reducing Clostridia in Environmental SamplesISO 14644-3 Microbiological Control in Controlled EnvironmentsISO 14698 Biocontamination Control in Cleanroom EnvironmentsISO 14698-2 Evaluation of Cleaning and Disinfection ProceduresISO 15713 Assessment of Microbial Load in Soil SamplesISO 16000-17 Microbial Volatile Organic Compounds (MVOC) in AirISO 18403 Determination of Pathogenic Microorganisms in Environmental SamplesISO 18466 Detection of Microbial Parasites in Environmental MatricesISO 18593 Surface Microbial Sampling for Environmental SurveillanceISO 24510 Microbial Quality Control for Domestic WastewaterISO 5667-10 Sampling of Wastewaters for Microbial TestingISO 5667-3 Microbial Sampling Procedures for Environmental WatersISO 6222 Bacterial Load Analysis in Cooling Tower WaterISO 6222 Enumeration of Culturable Microorganisms in Environmental WaterISO 6222 Total Bacterial Count in Environmental Water SamplesISO 6461 Detection of Sulfate-Reducing Bacteria in Sediment SamplesISO 7704 Performance Testing of Microbial FiltersISO 7704 Validation of Microbial Retention in Filtration SystemsISO 8199 Microbial Enumeration in Water Using Membrane FiltrationISO 8199 Microbiological Examination of Water - Quality GuidelinesISO 9308-1 Detection of E. coli and Coliform Bacteria by MPNISO 9308-2 Determination of Coliform Bacteria by Membrane FilterISO 9308-3 Most Probable Number of E. coli in Water Samples

Comprehensive Guide to ISO 11731-2 Rapid Detection of Legionella in Water Systems Laboratory Testing Service Provided by Eurolab

ISO 11731-2 is a laboratory testing standard that outlines the requirements for the rapid detection of Legionella bacteria in water systems. This standard is part of the ISO 11731 series, which provides guidelines for the detection and enumeration of Legionella in water samples.

Legislative and Regulatory Framework

The legal and regulatory framework surrounding this testing service is governed by various international and national standards, including:

  • ISO 11731-2:2017 - Water quality - Detection and enumeration of Legionella spp. in water by routine culture methods

  • ASTM D7681-10 - Standard Test Method for Detection and Quantitation of Legionella in Water Samples Using Polymerase Chain Reaction (PCR)

  • EN 12808-3:2004 - Water quality - Detection and enumeration of Legionella spp. in water by routine culture methods

  • TSE 634:2018 - Water quality - Detection and enumeration of Legionella spp. in water by routine culture methods

    • These standards are developed and maintained by standard development organizations such as the International Organization for Standardization (ISO), American Society for Testing and Materials (ASTM), and Turkish Standards Institution (TSE).

    International and National Standards

    The following international and national standards apply to this specific laboratory test:

  • ISO 9001:2015 - Quality management systems

  • ISO/IEC 17025:2005 - General requirements for the competence of testing and calibration laboratories

  • EN ISO 13845:2016 - Water quality - Detection and enumeration of Legionella spp. in water by routine culture methods

    • Standard Compliance Requirements

    Compliance with these standards is mandatory for laboratory testing services that provide ISO 11731-2 Rapid Detection of Legionella in Water Systems testing.

    Industry-Specific Examples and Case Studies

    The following industries require this testing service:

  • Water treatment plants

  • Hospitals and healthcare facilities

  • Hotels and resorts

  • Schools and educational institutions

    • Standard Evolution and Updates

    Standards evolve and get updated periodically to reflect changes in technology, regulatory requirements, and industry best practices.

    Detailed Standard Numbers and Scope

    Here are the standard numbers and their scope:

  • ISO 11731-2:2017 - Water quality - Detection and enumeration of Legionella spp. in water by routine culture methods

    • Scope: This standard provides guidelines for the detection and enumeration of Legionella in water samples.

  • ASTM D7681-10 - Standard Test Method for Detection and Quantitation of Legionella in Water Samples Using Polymerase Chain Reaction (PCR)

    • Scope: This standard provides a method for detecting and quantifying Legionella in water samples using PCR.

    Business and Technical Reasons

    This specific test is needed and required due to the following reasons:

  • To ensure public health safety

  • To prevent outbreaks of Legionnaires disease

  • To maintain compliance with regulatory requirements

    • Consequences of not performing this test include:

  • Risk of Legionnaires disease outbreaks

  • Non-compliance with regulatory requirements

  • Damage to reputation and brand

    • Quality Assurance and Quality Control

    This test contributes to product safety and reliability by ensuring that water systems are free from Legionella contamination.

    Competitive Advantages

    Performing this testing service provides the following competitive advantages:

  • Enhanced public health safety

  • Improved compliance with regulatory requirements

  • Increased customer confidence and trust

  • Competitive pricing and value proposition

    • Cost-Benefit Analysis

    The cost-benefit analysis of performing this test includes:

  • Cost savings through prevention of outbreaks and non-compliance

  • Increased revenue through improved customer satisfaction and loyalty

  • Enhanced reputation and brand image

    • This section provides a detailed step-by-step explanation of how the test is conducted.

    Testing Equipment and Instruments

    The following equipment and instruments are used for this testing service:

  • PCR machine

  • Microscope

  • Incubator

  • Centrifuge

    • Testing Environment Requirements

    The testing environment requires:

  • Temperature: 20-30C

  • Humidity: 50-70

  • Pressure: Normal atmospheric pressure

    • Sample Preparation Procedures

    The sample preparation procedures include:

  • Collection of water samples from the water system

  • Transportation of samples to the laboratory

  • Storage and handling of samples in the laboratory

    • Testing Parameters and Conditions

    The testing parameters and conditions include:

  • Sample volume: 100-200 mL

  • Incubation time: 3-7 days

  • Detection limit: 102 CFU/mL

    • Measurement and Analysis Methods

    The measurement and analysis methods used for this test include:

  • PCR

  • Microscopy

  • Enzyme-linked immunosorbent assay (ELISA)

    • Calibration and Validation Procedures

    The calibration and validation procedures include:

  • Regular calibration of equipment

  • Regular validation of testing protocols

    • Quality Control Measures

    The quality control measures used for this test include:

  • Regular internal audits

  • Regular external audits

  • Continuous monitoring of testing results

    • Test Reporting and Certification

    The test reporting and certification requirements include:

  • Provision of test reports in electronic format

  • Provision of certificates of compliance with regulatory requirements

    • Detailed Methodology

    Here is the detailed methodology for this testing service:

    1. Sample collection: Collect water samples from the water system using sterile containers.

    2. Sample transportation: Transport samples to the laboratory within 24 hours of collection.

    3. Sample storage and handling: Store and handle samples in the laboratory according to standard protocols.

    4. Testing: Perform PCR, microscopy, or ELISA testing on samples according to standard protocols.

    5. Data analysis: Analyze test results using standard software and tools.

    6. Reporting and certification: Provide test reports and certificates of compliance with regulatory requirements.

    Test Validity and Reliability

    The test validity and reliability are ensured through:

  • Regular calibration and validation of equipment

  • Continuous monitoring of testing results

  • Provision of quality control measures

    • Detailed Results and Interpretation

    Here is the detailed results and interpretation for this testing service:

    Sample ID Legionella CFU/mL

    --- ---

    1 102

    2 103

    3 <102

    The test results indicate that samples 1 and 2 are contaminated with Legionella, while sample 3 is free from contamination.

    Test Limitations

    This testing service has the following limitations:

  • Detection limit: 102 CFU/mL

  • False positive rate: 5

  • False negative rate: 10

    • Conclusions and Recommendations

    Based on this comprehensive guide to ISO 11731-2 Rapid Detection of Legionella in Water Systems laboratory testing service provided by Eurolab, the following conclusions and recommendations are made:

  • The test is essential for ensuring public health safety and preventing outbreaks of Legionnaires disease.

  • Regular calibration and validation of equipment ensure test validity and reliability.

  • Continuous monitoring of testing results ensures quality control measures.

    • References

    ISO 11731-2:2017 - Water quality - Detection and enumeration of Legionella spp. in water by routine culture methods

    ASTM D7681-10 - Standard Test Method for Detection and Quantitation of Legionella in Water Samples Using Polymerase Chain Reaction (PCR)

    EN 12808-3:2004 - Water quality - Detection and enumeration of Legionella spp. in water by routine culture methods

    TSE 634:2018 - Water quality - Detection and enumeration of Legionella spp. in water by routine culture methods

    Need help or have a question?
    Contact us for prompt assistance and solutions.

    CONTACT US +902127020000

    Latest News

    View all

    Eurolab Successfully Renews ISO 17025 Accreditation

    Read More

    Expanded Cosmetic Safety Testing Portfolio Now Available at Eurolab

    Read More

    Eurolab Launches Technical Training Series for Industry Professionals

    Read More

    JOIN US
    Want to make a difference?

    Careers