EUROLAB
iso-24271-gmo-screening-using-real-time-pcr-in-soy-based-products
GMO Testing AOAC 2005.01 Multiplex PCR for GMO Event Detection in MaizeAOAC 2007.01 GMO Screening in Corn-Based SnacksAOAC 2009.01 Detection of Roundup Ready Soybean in Food ProductsAOAC 2010.02 GMO DNA Quantification in Canola Oil ProductsAOAC 2010.03 GMO Identification in Cereal-Based Food ProductsAOAC 2011.04 GMO Identification in Processed Foods by PCRAOAC 2012.02 GMO Quantification in Vegetable Protein PowdersAOAC 2013.01 GMO Identification in Meat and Poultry FeedAOAC 2014.05 GMO Testing in Processed Dairy ProductsAOAC 2015.01 Detection of GM Canola in Oil and Meal ProductsAOAC 2016.03 GMO Screening in Wheat and DerivativesAOAC 2017.05 GMO Testing in Infant Formula and Baby FoodAOAC 2018.04 GMO Detection in Fish and Seafood FeedAOAC Official Method for GMO Screening in Mixed Grain ProductsCodex Alimentarius GMO Risk Assessment and Testing ProceduresCodex Alimentarius Guidelines for GMO Labeling TestingCodex GMO Sampling and Testing Guidelines for Food SafetyCodex GMO Testing Procedures for Labeling ComplianceEU Commission Decision 2012/540 GMO Reference Materials TestingEU Official Methods for GMO Testing in Food Supply ChainsEU Reference Laboratory Protocol for GMO Testing in Imported FoodsEU Regulation 1830/2003 Compliance Testing for GMO TraceabilityEU Regulation 1830/2003 GMO Traceability and Testing in Food ChainsEU Regulation 1830/2003 Labeling and GMO Testing in Infant FoodsEU Regulation No 1829/2003 GMO Testing Compliance for Food ImportsISO 21568 GMO Detection in Rice Products by ELISA and PCRISO 21568 GMO Event Detection in Biofuel Raw MaterialsISO 21568 GMO Quantification in Fermented Food ProductsISO 21568 GMO Screening and Quantification in Meat ProductsISO 21568 GMO Screening for Herbicide-Tolerant CropsISO 21569 GMO Detection in Food and Feed ProductsISO 21569 GMO Detection in Tomato and Derivative ProductsISO 21569 GMO Screening in Pulse and Legume CropsISO 21569 GMO Testing in Bakery and Confectionery ProductsISO 21570 Detection of GM Papaya in Fresh ProduceISO 21570 DNA Extraction Methods for GMO Analysis in CropsISO 21570 Event-Specific PCR for GMO Monitoring in Cotton SeedsISO 21570 GMO Detection in Processed Fruits and VegetablesISO 21570 GMO Presence Analysis in Vegetable OilsISO 21570 GMO Quantification in Baby Food FormulationsISO 21570 PCR-Based GMO Event Detection in Sugar CaneISO 21571 GMO Detection in Processed Food Using Digital PCRISO 21571 GMO Identification in Edible Oils and FatsISO 21571 Real-Time PCR GMO Quantification in Processed FoodsISO 21571 Validation of GMO Detection Methods in Food IndustryISO 24276 GMO Event Quantification in Sugar Beet ProductsISO 24276 GMO Quantification in Animal Feed MaterialsISO 24276 Multiplex PCR Assays for GMO Detection in Mixed CropsISO 24276 Quantitative PCR for GMO Content in Corn and SoyISO 24276 Quantitative PCR for GMO Detection in Processed Snacks

ISO 24271 GMO Screening Using Real-Time PCR in Soy-Based Products: Eurolabs Laboratory Testing Service

The testing of genetically modified organisms (GMOs) in soy-based products is governed by various international and national standards. ISO 24271, titled Molecular biological detection of genetically modified organisms (GMOs) provides a framework for the detection of GMOs using real-time polymerase chain reaction (PCR).

Relevant Standards:

  • ISO 24271:2015 - Molecular biological detection of genetically modified organisms (GMOs)
  • ASTM E2653-11 - Standard Guide for PCR-Based Detection and Quantitation of Genetically Modified Organisms in Food
  • EN ISO/IEC 17025:2005 - General requirements for the competence of testing and calibration laboratories
  • TSE (Turkish Standards Institution) 1002:2014 - Molecular biological detection of genetically modified organisms (GMOs)
  • Standard Development Organizations:

    The International Organization for Standardization (ISO) is responsible for developing and publishing ISO 24271. The European Committee for Standardization (CEN) and the Turkish Standards Institution (TSE) have adopted this standard, while ASTM has developed a similar guide.

    Standard Evolution:

    Standards evolve over time to address new technologies, methodologies, and regulatory requirements. Updates are made through a consensus-based process involving experts from industry, academia, and government.

    Specific Standard Numbers and Scope:

  • ISO 24271:2015 - Molecular biological detection of genetically modified organisms (GMOs)
  • Scope: This standard provides a framework for the molecular biological detection of GMOs using real-time PCR.

  • ASTM E2653-11 - Standard Guide for PCR-Based Detection and Quantitation of Genetically Modified Organisms in Food
  • Scope: This guide provides recommendations for the use of PCR-based methods for detecting and quantifying GMOs in food.

    Standard Compliance Requirements:

    Compliance with these standards is essential for laboratories, manufacturers, and regulatory agencies to ensure the accuracy and reliability of GMO testing results. Industry-specific compliance requirements may vary depending on regional regulations and market demands.

    The legal and regulatory framework surrounding GMO testing is complex and evolving. Countries have implemented their own laws and regulations governing GMO labeling, detection, and approval. Laboratories must comply with these regulations to ensure the accuracy and reliability of their results.

    The need for ISO 24271 GMO Screening Using Real-Time PCR in Soy-Based Products testing arises from several business and technical reasons:

  • Regulatory Compliance: Manufacturers must comply with labeling regulations, which require accurate detection and quantification of GMOs.
  • Product Safety: Unintended consequences of GMO introduction into the food chain can have significant health implications. Testing ensures that products meet safety standards.
  • Market Access: Manufacturers may face trade barriers if their products do not meet regulatory requirements in importing countries.
  • Risk Assessment: Testing helps manufacturers identify potential risks associated with GMOs, enabling them to mitigate these risks.
  • Consequences of Not Performing this Test:

    Failure to perform ISO 24271 GMO Screening Using Real-Time PCR in Soy-Based Products testing can result in:

  • Non-compliance with labeling regulations
  • Product recalls or withdrawal from the market
  • Loss of market access and reputation damage
  • Increased costs associated with corrective actions
  • Industries and Sectors Requiring this Testing:

    Manufacturers of soy-based products, including food and animal feed, must comply with regulatory requirements for GMO detection.

    Risk Factors and Safety Implications:

    GMOs can pose unintended health risks if not properly detected and managed. Manufacturers must ensure that their products meet safety standards to avoid potential liabilities.

    Quality Assurance and Quality Control Aspects:

    Laboratories must adhere to strict quality control measures, including standard operating procedures (SOPs), calibration, and validation of equipment to ensure accurate results.

    Competitive Advantages and Cost-Benefit Analysis:

    Performing ISO 24271 GMO Screening Using Real-Time PCR in Soy-Based Products testing provides manufacturers with a competitive advantage by:

  • Ensuring compliance with regulatory requirements
  • Mitigating potential risks associated with GMOs
  • Enhancing product safety and reliability
  • The cost-benefit analysis of performing this test is favorable, as it can prevent costly recalls, reputational damage, and lost market access.

    To maintain industry competitiveness, manufacturers must comply with evolving regulatory requirements. The benefits of ISO 24271 GMO Screening Using Real-Time PCR in Soy-Based Products testing far outweigh the costs associated with its implementation.

    The test is conducted using real-time PCR technology to detect and quantify GMOs in soy-based products.

    Testing Equipment and Instruments:

  • Real-time PCR machines (e.g., ABI 7500, QuantStudio)
  • DNA extraction kits
  • Primers and probes specific for the target GMO
  • DNA Extraction:

    The first step involves extracting DNA from the sample using a commercial kit or in-house protocol. The extracted DNA is then purified to remove contaminants.

    PCR Reaction:

    The purified DNA is subjected to real-time PCR, where the primers and probes specific for the target GMO are used to amplify and detect the target sequence.

    Data Analysis:

    The resulting data are analyzed using software provided by the manufacturer or in-house analysis tools to determine the presence and quantity of the target GMO.

    The test conditions and methodology are critical to ensure accurate results. Laboratories must adhere to strict quality control measures, including standard operating procedures (SOPs), calibration, and validation of equipment.

    Interpretation of Results:

    The interpretation of results is based on the presence or absence of the target GMO sequence. The quantity of the target GMO can be determined using quantitation protocols.

    To ensure accurate results, laboratories must follow strict quality control measures, including standard operating procedures (SOPs), calibration, and validation of equipment.

    Interpretation of Results:

    The interpretation of results is based on the presence or absence of the target GMO sequence. The quantity of the target GMO can be determined using quantitation protocols.

    To ensure accurate results, laboratories must follow strict quality control measures, including standard operating procedures (SOPs), calibration, and validation of equipment.

    Interpretation of Results:

    The interpretation of results is based on the presence or absence of the target GMO sequence. The quantity of the target GMO can be determined using quantitation protocols.

    To ensure accurate results, laboratories must follow strict quality control measures, including standard operating procedures (SOPs), calibration, and validation of equipment.

    Interpretation of Results:

    The interpretation of results is based on the presence or absence of the target GMO sequence. The quantity of the target GMO can be determined using quantitation protocols.

    Conclusion:

    ISO 24271 GMO Screening Using Real-Time PCR in Soy-Based Products testing is a critical step in ensuring compliance with regulatory requirements, mitigating potential risks associated with GMOs, and enhancing product safety and reliability. Manufacturers must adhere to strict quality control measures to ensure accurate results.

    Eurolabs Laboratory Testing Service:

    Eurolab offers a comprehensive laboratory testing service for ISO 24271 GMO Screening Using Real-Time PCR in Soy-Based Products. Our expert team ensures that all samples are analyzed using state-of-the-art equipment and techniques, guaranteeing accurate and reliable results.

    Contact Us:

    For more information about Eurolabs laboratory testing service or to discuss your specific testing needs, please contact us at:

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