EUROLAB
iso-24276-multiplex-pcr-assays-for-gmo-detection-in-mixed-crops
GMO Testing AOAC 2005.01 Multiplex PCR for GMO Event Detection in MaizeAOAC 2007.01 GMO Screening in Corn-Based SnacksAOAC 2009.01 Detection of Roundup Ready Soybean in Food ProductsAOAC 2010.02 GMO DNA Quantification in Canola Oil ProductsAOAC 2010.03 GMO Identification in Cereal-Based Food ProductsAOAC 2011.04 GMO Identification in Processed Foods by PCRAOAC 2012.02 GMO Quantification in Vegetable Protein PowdersAOAC 2013.01 GMO Identification in Meat and Poultry FeedAOAC 2014.05 GMO Testing in Processed Dairy ProductsAOAC 2015.01 Detection of GM Canola in Oil and Meal ProductsAOAC 2016.03 GMO Screening in Wheat and DerivativesAOAC 2017.05 GMO Testing in Infant Formula and Baby FoodAOAC 2018.04 GMO Detection in Fish and Seafood FeedAOAC Official Method for GMO Screening in Mixed Grain ProductsCodex Alimentarius GMO Risk Assessment and Testing ProceduresCodex Alimentarius Guidelines for GMO Labeling TestingCodex GMO Sampling and Testing Guidelines for Food SafetyCodex GMO Testing Procedures for Labeling ComplianceEU Commission Decision 2012/540 GMO Reference Materials TestingEU Official Methods for GMO Testing in Food Supply ChainsEU Reference Laboratory Protocol for GMO Testing in Imported FoodsEU Regulation 1830/2003 Compliance Testing for GMO TraceabilityEU Regulation 1830/2003 GMO Traceability and Testing in Food ChainsEU Regulation 1830/2003 Labeling and GMO Testing in Infant FoodsEU Regulation No 1829/2003 GMO Testing Compliance for Food ImportsISO 21568 GMO Detection in Rice Products by ELISA and PCRISO 21568 GMO Event Detection in Biofuel Raw MaterialsISO 21568 GMO Quantification in Fermented Food ProductsISO 21568 GMO Screening and Quantification in Meat ProductsISO 21568 GMO Screening for Herbicide-Tolerant CropsISO 21569 GMO Detection in Food and Feed ProductsISO 21569 GMO Detection in Tomato and Derivative ProductsISO 21569 GMO Screening in Pulse and Legume CropsISO 21569 GMO Testing in Bakery and Confectionery ProductsISO 21570 Detection of GM Papaya in Fresh ProduceISO 21570 DNA Extraction Methods for GMO Analysis in CropsISO 21570 Event-Specific PCR for GMO Monitoring in Cotton SeedsISO 21570 GMO Detection in Processed Fruits and VegetablesISO 21570 GMO Presence Analysis in Vegetable OilsISO 21570 GMO Quantification in Baby Food FormulationsISO 21570 PCR-Based GMO Event Detection in Sugar CaneISO 21571 GMO Detection in Processed Food Using Digital PCRISO 21571 GMO Identification in Edible Oils and FatsISO 21571 Real-Time PCR GMO Quantification in Processed FoodsISO 21571 Validation of GMO Detection Methods in Food IndustryISO 24271 GMO Screening Using Real-Time PCR in Soy-Based ProductsISO 24276 GMO Event Quantification in Sugar Beet ProductsISO 24276 GMO Quantification in Animal Feed MaterialsISO 24276 Quantitative PCR for GMO Content in Corn and SoyISO 24276 Quantitative PCR for GMO Detection in Processed Snacks

ISO 24276 Multiplex PCR Assays for GMO Detection in Mixed Crops: Eurolabs Laboratory Testing Service

Standard-Related Information

The detection of genetically modified organisms (GMOs) in mixed crops has become a pressing concern for industries involved in food production, processing, and trade. To address this need, the International Organization for Standardization (ISO) has developed ISO 24276:2019 Multiplex PCR Assays for GMO Detection in Mixed Crops. This standard provides a framework for the development of polymerase chain reaction (PCR)-based assays for the detection of GMOs in mixed crops.

Legal and Regulatory Framework

The detection of GMOs is regulated by various national and international laws, including:

  • EU Regulation 1830/2003 on the introduction of genetically modified food and feed

  • EFSAs GMO Panel Guidance Document for GMO Detection

  • USDA-APHIS regulations for GMO imports

    • Compliance with these regulations requires accurate and reliable testing methods, such as those specified in ISO 24276.

    Standard Development Organizations

    The development of standards like ISO 24276 involves collaboration among industry stakeholders, regulatory bodies, and standardization organizations. The main players include:

  • ISO (International Organization for Standardization)

  • ICS (International Committee on Standards)

  • ASTM (American Society for Testing and Materials)

  • EN (European Committee for Standardization)

    • Standard Evolution and Updates

    Standards evolve over time to reflect new technologies, methods, and regulatory requirements. ISO 24276:2019 is the latest revision of this standard, which has undergone several updates since its initial publication in 2006.

    Specific Standard Numbers and Scope

    The following standards are relevant to GMO detection in mixed crops:

  • ISO 24276:2019 Multiplex PCR Assays for GMO Detection in Mixed Crops

  • ISO 21570:2014 Polymerase chain reaction (PCR) - General guidelines

  • EN 14502-1:2005 Genetic modification (GM) - Molecular biology - Guidelines for the detection of genetically modified organisms

    • Standard Compliance Requirements

    Compliance with these standards is mandatory for industries involved in GMO detection, including:

  • Food and feed manufacturers

  • Agricultural biotechnology companies

  • Regulatory agencies

  • Laboratories providing testing services

    • Failure to comply can result in product recalls, legal penalties, and damage to reputation.

    Eurolabs expertise in ISO 24276 Multiplex PCR Assays for GMO Detection in Mixed Crops has been recognized through its accreditation by international organizations such as ILAC (International Laboratory Accreditation Cooperation) and EA (European Co-operation for Accreditation).

    Eurolab follows a strict quality management system, ensuring the highest level of accuracy and reliability in its testing services. Our state-of-the-art equipment and facilities, combined with our experienced personnel, enable us to provide high-quality results efficiently.

    Standard Requirements and Needs

    The specific requirements for GMO detection in mixed crops are driven by:

  • The need for accurate and reliable testing methods

  • Regulatory compliance obligations

  • Market demands for GMO-free products

  • International trade requirements

    • Failure to perform this test can result in product contamination, regulatory non-compliance, and reputational damage.

    Consequences of Not Performing This Test

    Not detecting GMOs in mixed crops can lead to:

  • Product recalls and economic losses

  • Regulatory penalties and fines

  • Damage to reputation and loss of market share

  • Unintended environmental releases

    • The benefits of performing this test include:

  • Ensuring product safety and quality

  • Complying with regulatory requirements

  • Maintaining a competitive edge in the market

  • Enhancing customer trust and confidence

    • Test Conditions and Methodology

    Eurolabs testing service involves the following steps:

    1. Sample preparation: Preparation of mixed crop samples for PCR analysis.

    2. PCR setup: Design and optimization of PCR primers and probes for GMO detection.

    3. Amplification and detection: PCR amplification and detection of target sequences using fluorescence-based methods (e.g., TaqMan or SYBR Green).

    4. Data analysis: Interpretation of PCR data to determine the presence or absence of GMOs.

    Testing Equipment and Instruments

    Eurolab uses state-of-the-art equipment, including:

  • Thermocyclers (e.g., Applied Biosystems 7500 Fast Real-Time PCR System)

  • Fluorescence readers (e.g., Applied Biosystems 7000 Series Real-Time PCR System)

    • Sample Preparation Procedures

    Samples are prepared according to the ISO 24276 standard, which includes:

  • Homogenization and DNA extraction

  • Quantification of DNA using spectrophotometry or other methods

    • Testing Parameters and Conditions

    The testing parameters include:

  • Temperature (denaturation: 95C; annealing: 60C)

  • Number of cycles (35-45 cycles)

  • Primer and probe concentrations

    • Data Analysis and Interpretation

    Eurolabs experienced personnel analyze the PCR data to determine the presence or absence of GMOs. The results are reported in a clear and concise manner, including:

  • Presence or absence of target sequences

  • Quantification of target sequences (if applicable)

    • Eurolab follows strict quality control measures to ensure accurate and reliable testing results, including:

  • Regular calibration and maintenance of equipment

  • Participation in proficiency tests (PTs) and interlaboratory comparisons (ILCs)

  • Use of certified reference materials (CRMs)

    • Conclusion

    In conclusion, Eurolabs ISO 24276 Multiplex PCR Assays for GMO Detection in Mixed Crops testing service is designed to meet the highest standards of accuracy, reliability, and regulatory compliance. Our expertise and state-of-the-art equipment ensure that our clients receive high-quality results efficiently.

    We invite you to contact us to discuss your specific needs and requirements for GMO detection in mixed crops.

    References

  • ISO 24276:2019 Multiplex PCR Assays for GMO Detection in Mixed Crops

  • EFSAs GMO Panel Guidance Document for GMO Detection

  • USDA-APHIS regulations for GMO imports

    • By choosing Eurolab as your partner for GMO detection, you can ensure compliance with regulatory requirements, maintain a competitive edge in the market, and enhance customer trust and confidence.

    Please do not hesitate to contact us if you require any further information or clarification on our services.

    Need help or have a question?
    Contact us for prompt assistance and solutions.

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