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aoac-200508-determination-of-vitamin-e-stability-during-storage
Shelf Life and Stability Testing AOAC 2001.02 Oxidative Stability Testing in Edible OilsAOAC 2001.05 Water Activity and Humidity Control in Stability TestingAOAC 2003.04 Oxidation Stability of Processed Fish ProductsAOAC 2004.07 Sensory Shelf Life of Confectionery ProductsAOAC 2004.08 Analysis of Volatile Compounds in Shelf Life TestingAOAC 2005.01 Microbial Challenge Testing for Shelf Life ExtensionAOAC 2006.04 Microbial Stability in Vacuum-Packaged FoodsAOAC 2006.05 Quantitative Yeast Analysis in Shelf Life StudiesAOAC 2007.04 Antioxidant Stability in Nutritional SupplementsAOAC 2010.01 Stability Testing of Dairy Products Under Refrigerated ConditionsAOAC 2011.02 Stability of Nutritional Components During StorageAOAC 2011.02 Stability Testing of Vitamin Complexes in SupplementsAOAC 2013.05 Analysis of Antioxidant Degradation in Food ProductsAOAC 960.52 Fat Oxidation Testing in Processed MeatsAOAC 960.52 Lipid Oxidation Measurement in Shelf Life StudiesAOAC 972.33 Lipid Oxidation Assessment in Snack FoodsAOAC 975.03 Ethanol Content Stability in Alcoholic BeveragesAOAC 981.14 Preservative Efficacy Testing in Meat ProductsAOAC 984.27 Stability Testing of Mineral Content in Food ProductsAOAC 985.29 Fiber Content Stability in Packaged FoodsAOAC 991.18 Determination of Moisture Content in Relation to StabilityAOAC 991.29 Stability Testing of Fatty Acids in Food MatricesAOAC 991.31 Sugar Content Stability in BeveragesAOAC 991.36 Caffeine Stability Testing in Energy DrinksAOAC 993.13 Shelf Life Testing of Fermented Dairy ProductsAOAC 994.12 Volatile Compound Analysis in Stored Fruit JuicesAOAC 995.10 Chemical Stability Analysis in BeveragesAOAC 996.06 Vitamin Degradation Testing During StorageAOAC 999.03 Total Plate Count Enumeration for Shelf Life MonitoringAOAC 999.10 Mycotoxin Stability Testing in Grain ProductsAOAC 999.12 Sensory Shelf Life Evaluation in Baked GoodsAOAC 999.14 Phosphorus Stability Testing in Dairy ProductsAOAC 999.15 Water Activity Measurement and Its Effect on StabilityASTM D4169 Distribution Simulation for Shelf Life PredictionASTM D4169 Impact Testing on Packaging for Product StabilityASTM D4169 Simulation Testing for Distribution and Shelf Life ImpactASTM D4169 Testing Packaging Resistance to Transportation for Shelf LifeASTM D4169 Vibration Testing Impact on Shelf Life of Fragile ProductsASTM D5276 Drop Test Impact on Packaging and Product StabilityASTM D5278 Environmental Stress Cracking Testing in PackagingASTM E1383 Shelf Life Testing of Adhesive Properties in Food LabelsASTM E1922 Texture Analysis for Shelf Life Quality ControlASTM E2305 Microbial Challenge Testing for Shelf Life DeterminationASTM E2709 Accelerated Aging of Food Packaging MaterialsASTM F1980 Predictive Shelf Life Modeling Using Accelerated AgingASTM F1980 Thermal Aging Testing for Shelf Life Estimation of Food PackagingASTM F1980 Thermal Stability Testing of Heat-Sensitive NutrientsASTM F2095 Testing of Gas Barrier Properties Affecting Shelf LifeASTM F2096 Gas Permeability Testing in Modified Atmosphere PackagingASTM F2254 Packaging Integrity Testing in Relation to Shelf LifeASTM F88 Seal Strength Testing for Packaging Affecting Shelf LifeISO 11133 Microbial Culture Preparation for Stability TestingISO 11133-1 Microbiological Culture Methods for Shelf Life TestingISO 11290 Detection of Listeria Monocytogenes in Shelf Life StudiesISO 11290 Listeria Monocytogenes Monitoring in Shelf Life TestingISO 11290-2 Detection of Foodborne Pathogens in Shelf Life TestingISO 11304 Analysis of Fatty Acid Stability During StorageISO 11307 Shelf Life Prediction Using Arrhenius Model for Frozen FoodsISO 11505 Temperature Cycling Testing for Frozen Ready MealsISO 11607 Packaging Validation for Shelf Life AssuranceISO 11799 Shelf Life Testing of Pharmaceutical Grade Nutritional ProductsISO 11885 Trace Metal Analysis Affecting Product StabilityISO 12099 Shelf Life Validation for Infant Formula ProductsISO 13720 Quality Assurance of Milk and Milk Products During StorageISO 1442 Moisture Content Testing in Shelf Life EvaluationsISO 14698 Bioburden and Contamination Control in Shelf Life StudiesISO 15214 Enumeration of Lactic Acid Bacteria in Shelf Life TestsISO 15705 Analysis of Preservatives in Shelf Life StudiesISO 16000 Volatile Organic Compounds Monitoring in Food StorageISO 16140 Validation of Microbiological Methods for Shelf LifeISO 16212 Controlled Atmosphere Storage Testing for Fresh ProduceISO 16732 Sensory Shelf Life Testing of Packaged SnacksISO 17025 Accredited Accelerated Shelf Life Testing of Packaged FoodsISO 17025 Accredited Shelf Life Testing of Gluten-Free ProductsISO 17994 Comparative Microbial Recovery Testing in Shelf Life StudiesISO 21326 Microbial Methods for Determining Shelf Life of Canned FoodsISO 21527 Yeast and Mold Enumeration During Storage Stability TestsISO 21527 Yeast and Mold Enumeration in Shelf Life TestingISO 21528-2 Enumeration of Coliforms in Food Stability StudiesISO 22000 Microbial Safety Assessment for Shelf Life ExtensionISO 22000 Microbial Stability Testing for Shelf Life Determination in Ready MealsISO 22005 Traceability and Shelf Life Monitoring in Food Supply ChainsISO 2232 Freeze-Thaw Stability Testing of Frozen Food ProductsISO 22718 Testing of Antimicrobial Effectiveness in Food PackagingISO 22964 Evaluation of Shelf Life for Fresh-Cut ProduceISO 24333 Shelf Life Testing of Ready-to-Eat Meat ProductsISO 24511 Evaluation of Storage Conditions on Microbial GrowthISO 26323 Sensory Shelf Life Testing of Fresh ProduceISO 4832 Enumeration of Enterobacteriaceae in Shelf Life EvaluationISO 4833 Enumeration of Microbial Load in Shelf Life DeterminationISO 4833 Enumeration of Microorganisms Relevant to Shelf LifeISO 6579 Detection of Salmonella During Product Stability AssessmentISO 6579 Microbiological Testing for Shelf Life in Ready-to-Eat MealsISO 6579-1 Salmonella Detection During Shelf Life TestingISO 6579-1 Salmonella spp. Detection in Shelf Life StudiesISO 660 Acid Value Testing in Oil Stability StudiesISO 6887 Sample Preparation Techniques for Shelf Life TestingISO 7303 Sensory Analysis Methods for Shelf Life MonitoringISO 9227 Corrosion Testing on Packaging Affecting Shelf Life

AOAC 2005.08 Determination of Vitamin E Stability During Storage Laboratory Testing Service: A Comprehensive Guide

The AOAC 2005.08 Determination of Vitamin E Stability During Storage laboratory testing service is governed by various international and national standards. The primary standard for this test is the AOAC International Official Method 992.03, which outlines the procedure for determining the stability of vitamin E in food products during storage.

International Standards:

  • ISO/IEC 17025:2005 General Requirements for the Competence of Testing and Calibration Laboratories
  • ASTM E2586-07 Standard Practice for Quality Control of Analytical Methods for Elemental Determinations
  • EN 14110:2010 Foodstuffs - Sampling and sample preparation
  • National Standards:

  • EU Directive 2008/28/EC on the protection of consumers rights in respect of unfair contract terms
  • FDA 21 CFR Part 101, Subpart A - General Provisions for Food Labeling
  • Standard Development Organizations:

  • AOAC International (AOAC)
  • International Organization for Standardization (ISO)
  • American Society for Testing and Materials (ASTM)
  • Evolution of Standards:

    Standards evolve through a rigorous review process by standard development organizations. The updates reflect new technologies, scientific discoveries, and regulatory requirements.

    Standard Numbers and Scope:

    Standard Number Title Scope

    --- --- ---

    AOAC 2005.08 Determination of Vitamin E Stability During Storage This method covers the determination of vitamin E stability in food products during storage.

    Industry-Specific Requirements:

    Food manufacturers, retailers, and regulatory agencies require this testing to ensure compliance with labeling regulations.

    Consequences of Non-Compliance:

    Failure to perform this test may result in product recalls, financial penalties, and damage to reputation.

    Standard Compliance Requirements:

    Industries requiring standard compliance include:

  • Food manufacturing
  • Retailing
  • Regulatory agencies
  • Business and Technical Reasons for Conducting AOAC 2005.08 Determination of Vitamin E Stability During Storage Testing:

  • Ensure product safety and reliability
  • Comply with labeling regulations
  • Maintain customer confidence and trust
  • Enhance competitive advantage through quality assurance
  • Consequences of Not Performing this Test:

  • Product recalls
  • Financial penalties
  • Damage to reputation
  • Industries and Sectors Requiring this Testing:

  • Food manufacturing
  • Retailing
  • Regulatory agencies
  • Risk Factors and Safety Implications:

  • Vitamin E instability may lead to health issues in consumers
  • Quality Assurance and Quality Control Aspects:

  • Calibration and validation of equipment
  • Sample preparation procedures
  • Measurement and analysis methods
  • Contributions to Product Safety and Reliability:

    This test ensures that vitamin E levels remain stable during storage, preventing potential health risks.

    Competitive Advantages through Quality Assurance:

    By conducting this testing, companies demonstrate their commitment to quality and customer safety.

    Cost-Benefit Analysis:

    Performing this test provides long-term benefits through enhanced reputation, increased customer trust, and reduced risk of product recalls.

    Step-by-Step Explanation of the Test Conducted:

    1. Sample collection

    2. Preparation of samples for analysis

    3. Determination of vitamin E stability using high-performance liquid chromatography (HPLC)

    Testing Equipment and Instruments Used:

  • HPLC system
  • Autosampler
  • Column oven
  • Testing Environment Requirements:

  • Temperature control: 20 5C
  • Humidity control: 50 10
  • Pressure control: 1 atm
  • Sample Preparation Procedures:

  • Homogenization of samples
  • Extraction of vitamin E from samples using a solvent
  • Testing Parameters and Conditions:

  • HPLC column: C18 (250 4.6 mm)
  • Mobile phase: Methanol/water (90/10, v/v)
  • Flow rate: 1 mL/min
  • Detection wavelength: 290 nm
  • Measurement and Analysis Methods:

  • Quantification of vitamin E using external standard method
  • Calibration and Validation Procedures:

  • Regular calibration of HPLC system
  • Validation of analytical method through recovery studies
  • Quality Control Measures During Testing:

  • Verification of equipment performance
  • Monitoring of testing environment conditions
  • Data Collection and Recording Procedures:

  • Automatic data collection using software
  • Manual recording of results in laboratory notebook
  • Testing Timeframes and Duration:

  • Sample preparation: 1-2 hours
  • Testing: 4-6 hours
  • Total duration: 5-8 hours
  • Sample Size Requirements and Statistical Considerations:

  • Minimum sample size: 10 replicates per sample
  • Statistical analysis using ANOVA
  • Determination of Vitamin E Stability During Storage:

    This method measures the stability of vitamin E in food products during storage.

    High-Performance Liquid Chromatography (HPLC):

    The HPLC system is used to separate, identify, and quantify vitamin E.

    Column Oven:

    Temperature control ensures optimal separation of vitamin E from other compounds.

    Autosampler:

    Automated injection of samples reduces manual errors.

    Reagents and Solvents:

  • Methanol
  • Water
  • Vitamin E standard
  • Preparation of Samples for Analysis:

    Samples are homogenized, extracted using a solvent, and filtered to remove particulate matter.

    Determination of Vitamin E Stability:

    Vitamin E stability is determined by measuring the peak area or height using external standards.

    Interpretation of Results:

    Results are expressed as percentage retention of vitamin E during storage.

    Conclusion:

    The AOAC 2005.08 Determination of Vitamin E Stability During Storage laboratory testing service ensures that food products remain safe for consumption. By following this method, companies can maintain customer trust and comply with labeling regulations.

    Certification and Accreditation:

    AOAC International is accredited by the American National Standards Institute (ANSI) for developing standards.

    Quality Management System (QMS):

    Companies performing this testing must implement a QMS to ensure compliance with standard requirements.

    Conclusion:

    The AOAC 2005.08 Determination of Vitamin E Stability During Storage laboratory testing service is essential for maintaining customer trust and complying with labeling regulations. Companies should perform this test regularly to ensure product safety and reliability.

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