AOAC 2011.25 Determination of DON (Deoxynivalenol) in Feed

AOAC 2011.25 Determination of DON (Deoxynivalenol) in Feed: Eurolabs Laboratory Testing Service

Standard-Related Information

The AOAC 2011.25 determination of DON (Deoxynivalenol) in feed is a widely recognized laboratory testing method for the detection and quantification of this mycotoxin in animal feed. This test is governed by several international standards, including:

These standards provide the framework for the testing process, ensuring that laboratories like Eurolab conduct the test with accuracy and precision.

Legal and Regulatory Framework

The detection of DON in animal feed is crucial due to its potential impact on human health. Deoxynivalenol can cause nausea, vomiting, and diarrhea in humans who consume contaminated meat or dairy products derived from animals fed DON-contaminated feed. In response to these risks, regulatory agencies worldwide have established guidelines for maximum permissible levels of DON in animal feed.

In the European Union, for example, the maximum acceptable level of DON is 1 mg/kg (EN 15282:2009). Similarly, the US FDA has set a tolerance limit of 5 ppm (ASTM D6888-10).

International and National Standards

The development and maintenance of standards for laboratory testing are typically handled by standard development organizations (SDOs) such as:

These SDOs work together to ensure that laboratory tests, including the AOAC 2011.25 determination of DON in feed, meet international standards.

Standard Evolution and Updates

Standards evolve as new technologies and scientific knowledge emerge. When updates are made to a standard, laboratories like Eurolab must adapt their testing methods to comply with the revised requirements.

For instance, the introduction of new extraction techniques or chromatography instruments may necessitate changes to sample preparation procedures or measurement conditions.

Standard Numbers and Scope

The following standards govern the AOAC 2011.25 determination of DON in feed:

Each standard outlines specific requirements for sample preparation, measurement conditions, and reporting.

Standard Compliance Requirements

Industry sectors that require this testing include:

Failure to comply with standards can result in product recalls, fines, or even legal action.

Why This Test is Needed and Required

The AOAC 2011.25 determination of DON in feed is necessary due to the risks associated with mycotoxin contamination. Deoxynivalenol can cause economic losses for farmers and producers, as well as pose a threat to human health.

Conducting this test ensures that animal feed meets regulatory requirements, minimizing the risk of product recalls or other consequences.

Business and Technical Reasons

Performing the AOAC 2011.25 determination of DON in feed provides several benefits:

Consequences of Not Performing This Test

Failure to conduct the AOAC 2011.25 determination of DON in feed can result in:

Test Conditions and Methodology

The AOAC 2011.25 determination of DON in feed involves the following steps:

1. Sample preparation: Homogenize the feed sample, extract DON using a suitable solvent.

2. Measurement conditions: Use high-performance liquid chromatography (HPLC) or gas chromatography (GC) to detect and quantify DON.

3. Calibration and validation: Validate the testing method against certified reference materials.

Test Reporting and Documentation

Eurolabs laboratory reporting system includes:

Conclusion

The AOAC 2011.25 determination of DON in feed is a critical laboratory testing service that ensures animal feed meets regulatory requirements. By complying with international standards, Eurolabs customers can minimize the risk of product recalls and economic losses while ensuring the safety of their products.