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en-16214-gmo-genetically-modified-organism-screening-in-feed-ingredients
Feed Analysis 6282 Feed Additive Compliance TestingAACC 08-01.01 Moisture Determination in Grain-Based FeedAACC 32-07.01 Alpha-Amylase Activity in Feed with Cereal BaseAACC 44-15.02 Water Activity in Feed Pellets and PowdersAACC 46-12.01 Oil Content in Oilseed Feed by Soxhlet ExtractionAOAC 2000.03 Fumonisin B1 and B2 in Corn-Based FeedAOAC 2003.01 Ochratoxin A Testing in Feed ProductsAOAC 2011.25 Determination of DON (Deoxynivalenol) in FeedAOAC 942.05 Salt (NaCl) Content in Feed by TitrationAOAC 969.33 Mycotoxin Aflatoxin B1 Testing in Feed SamplesAOAC 973.18 Determination of Copper and Zinc in Feed by AASAOAC 985.01 Fiber Content Determination in Feed Using Enzymatic MethodAOAC 986.15 Selenium Content Determination in FeedAOAC 994.12 Crude Fat Determination in Animal FeedAOAC 999.07 Zearalenone Detection in Animal FeedAOAC 999.10 Lead, Cadmium and Arsenic in Animal FeedASTM D1971 Silage Composition Testing for Forage-Based FeedASTM D2974 Organic and Inorganic Matter in Feed by CombustionASTM D3173 Volatile Matter Content in Animal Feed PelletsASTM D4574 Testing Water Soluble Phosphates in FeedASTM D4806 Ethanol Content in Fermented Feed SupplementsASTM D6007 VOC Emissions in Enclosed Feed StorageASTM E829 Sampling Method for Bulk Agricultural FeedBulk Density Measurement of Feed Raw MaterialsCodex CXG 71-2009 Hygienic Practices for Feed Manufacturing FacilitiesCodex CXG 80-2020 Mycotoxin Risk Management in FeedCodex STAN 192-1995 Feed Additive Maximum Limit Compliance TestCodex STAN 193-1995 Veterinary Drug Residue Limits in Feed IngredientsDetection of Ruminant Material in Non-Ruminant FeedDetermination of Urea in Protein-Rich FeedEC 152/2009 Feed Sampling and Analytical MethodsEC Regulation 1831/2003 Additive Authorization in FeedEC Regulation 767/2009 Labeling and Composition Requirements for FeedEN 15510 Determination of Trace Elements in Feed by ICP-MSEN 15559 Determination of Additive Concentrations in FeedEN 15634 Screening of Dioxins and Furans in FeedEN 15763 Quantification of Lysine, Methionine and Threonine in FeedEN 15781 Measurement of Pesticide Residues in Feed by LC-MS/MSFEDIAF Energy Content Analysis in Dog and Cat FeedFEDIAF Nutritional Composition Compliance Testing for Pet FeedFeed Homogeneity Analysis Using Mixing IndexISO 10694 Carbon and Nitrogen Analysis in Feed Using Combustion MethodISO 5983-2 Crude Protein Analysis in Feed by Kjeldahl MethodISO 5984 Ash Content Determination in Animal FeedISO 6491 Determination of Phosphorus in Compound FeedsISO 6496 Moisture Content Determination in Animal FeedISO 6865 Determination of Crude Fiber in Feed SamplesISO 6869 Calcium and Magnesium Determination in Feed by Atomic AbsorptionMicrobiological Quality Testing of Animal FeedOECD TG 223 Avian Dietary Toxicity Test for Poultry FeedOECD TG 442 Metabolite Bioavailability Testing in Animal FeedOECD TG 507 Feed Contaminant Assessment ProtocolPelleting Durability Test for Compound FeedSieve Analysis of Granulated Animal FeedUS EPA Method 200.8 Trace Metal Analysis by ICP-MS in FeedUS EPA Method 3050B Digestion for Trace Metals in Feed SamplesUSP 39 Loss on Drying Determination in Feed SupplementsUSP 41 Particle Size Distribution in Powdered Feed ProductsVitamin A, D and E Content in Feed

EN 16214 GMO (Genetically Modified Organism) Screening in Feed Ingredients: A Comprehensive Guide

Standard-Related Information

EN 16214 is a European standard that outlines the requirements for GMO screening in feed ingredients. This standard is developed by CEN (Comité Européen de Normalisation), which is responsible for creating harmonized standards across Europe. EN 16214 is based on ISO/TS 22003, an international standard for laboratory testing of GMOs.

Legal and Regulatory Framework

The legal and regulatory framework surrounding GMO screening in feed ingredients is governed by various national and international regulations. The most significant ones include:

  • EU Regulation (EC) No 1829/2003 on genetically modified food and feed

  • EU Regulation (EC) No 1830/2003 on genetically modified organisms (GMOs)

  • International Organization for Standardization (ISO) 22003:2018

    • These regulations require GMO screening to ensure the safety of genetically modified organisms used in feed ingredients.

    International and National Standards

    The following international and national standards apply to EN 16214 GMO (Genetically Modified Organism) Screening in Feed Ingredients testing:

  • ISO/TS 22003:2018 for laboratory testing of GMOs

  • EN 16214:2020 for GMO screening in feed ingredients

  • EU Regulation (EC) No 1829/2003 and EU Regulation (EC) No 1830/2003

    • Standard Development Organizations

    CEN is responsible for developing and maintaining European standards, including EN 16214. Other standard development organizations involved in the creation of this standard include:

  • ISO (International Organization for Standardization)

  • ASTM (American Society for Testing and Materials)

    • How Standards Evolve and Get Updated

    Standards evolve through a continuous process of review, revision, and update. This ensures that standards remain relevant and aligned with industry needs.

    Standard Numbers and Scope

    EN 16214:2020 provides the scope and requirements for GMO screening in feed ingredients. The standard number and scope are:

  • Standard Number: EN 16214

  • Title: Genetically Modified Organism (GMO) Screening in Feed Ingredients

  • Scope: This standard specifies the methods for detecting genetically modified organisms (GMOs) in feed ingredients.

    • Standard Compliance Requirements

    Industry sectors that require GMO screening include:

  • Animal feed manufacturers

  • Food manufacturers

  • Biotechnology companies

    • Conformity to EN 16214 ensures compliance with EU regulations and national standards, as well as provides a level of assurance for customers regarding the safety of genetically modified organisms used in feed ingredients.

  • Risk Factors: GMO contamination can result from various sources, including:

    • Improper sample handling

    Inadequate testing protocols

    Lack of quality control measures

    Business and Technical Reasons for Conducting EN 16214 GMO (Genetically Modified Organism) Screening in Feed Ingredients Testing

    Conducting EN 16214 GMO (Genetically Modified Organism) screening is essential to ensure the safety of genetically modified organisms used in feed ingredients. This test provides a level of assurance regarding product quality and regulatory compliance.

    Why this Specific Test is Needed and Required

    EN 16214 GMO (Genetically Modified Organism) screening is necessary due to:

  • Risk Assessment: GMO contamination can result from various sources, including:

    • Improper sample handling

    Inadequate testing protocols

    Lack of quality control measures

    Test Conditions and Methodology

    The EN 16214 test involves the following steps:

    1. Sample preparation: The feed ingredient is prepared according to the manufacturers instructions.

    2. Testing equipment and instruments:

    Real-Time PCR (Polymerase Chain Reaction) machine

    Microplate reader

    3. Testing environment requirements:

    Temperature: 20-25C

    Humidity: 40-60

    4. Sample preparation procedures:

    Sample Preparation Procedures

  • DNA Extraction: The feed ingredient is ground and mixed with a DNA extraction buffer.

  • Real-Time PCR: The extracted DNA is then analyzed using real-time PCR.

    • Testing Parameters and Conditions

    The testing parameters and conditions for EN 16214 GMO (Genetically Modified Organism) screening are:

  • Real-Time PCR: Temperature range of 55-60C

  • Microplate reader: Fluorescence measurement

    • Measurement and Analysis Methods

    The measurement and analysis methods used in EN 16214 GMO (Genetically Modified Organism) screening include:

  • Real-Time PCR: Quantitative analysis of genetically modified organisms

  • Microplate reader: Fluorescence measurement for quantification

    • Calibration and Validation Procedures

    The calibration and validation procedures for EN 16214 GMO (Genetically Modified Organism) screening involve the following steps:

    1. Calibration: The real-time PCR machine is calibrated according to the manufacturers instructions.

    2. Validation: The test method is validated using reference materials.

    Quality Control Measures

    EN 16214 GMO (Genetically Modified Organism) screening involves quality control measures, including:

  • Sample handling: Proper sample handling and storage

  • Testing protocols: Adherence to established testing protocols

    • Test Report and Certificate of Analysis

    The test report for EN 16214 GMO (Genetically Modified Organism) screening includes the following information:

  • Test results: Quantitative analysis of genetically modified organisms

  • Certificate of analysis: Confirmation that the feed ingredient meets regulatory requirements

    • Conclusion

    EN 16214 GMO (Genetically Modified Organism) screening is an essential test for ensuring the safety of genetically modified organisms used in feed ingredients. This standard provides a level of assurance regarding product quality and regulatory compliance.

    Why Choose Our Laboratory?

    Our laboratory offers EN 16214 GMO (Genetically Modified Organism) screening services with:

  • High-quality testing equipment

  • Experienced technicians

  • Comprehensive test report

    • Please contact us for more information on our EN 16214 GMO (Genetically Modified Organism) screening services.

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    Contact us for prompt assistance and solutions.

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