EUROLAB
aocs-official-method-ba-13-75-gmo-quantification-via-dpcr
GMO & Seed Purity Testing AFNOR NF V03-010 Interpretation of GMO PCR ResultsAFNOR XP V03-020 GMO Analytical Detection Method ValidationAFNOR XP V03-021 Reference Materials for GMO TestingAOAC 2006.01 Roundup Ready Canola GMO QuantificationAOAC 2008.04 GMO Testing for Bt CornAOAC 2013.01 Qualitative DNA Detection for GM CanolaAOAC 2016.04 GMO Screening in Corn and Soy ProductsAOCS Ba 10-65 Impurity Content in Seed SamplesAOCS Ba 11-65 Foreign Matter in Oilseed SamplesAOCS Ba 8-75 Mechanical Purity Determination in SeedsAOCS Ba 9-58 Seed Purity Testing in OilseedsAOCS Official Method Ba 12-75 Detection of GMO in LinseedCEN/TS 16707:2015 Qualitative Detection of GM CottonEN 13791 GMO Quantification Method for Maize Bt11EN 13835 GMO Detection – Roundup Ready SoyEN 13837 GMO – Event-Specific Detection for MON810EN 16707 Detection of Specific GMO Events in Soya and MaizeEN 16707-1 Detection of GMO Events in FlaxseedEN ISO 21569-4 Real-Time PCR Event Specific GMO DetectionEN ISO 21569-6 Digital PCR Detection of GM MaizeEN ISO 21571-3 GMO DNA Extraction from Oil-Rich SeedsEN ISO 21572 Protein-Based Methods for GMO DetectionEN ISO 21573 Guidelines for GMO Testing LaboratoriesEN ISO 24276 General Laboratory Requirements for GMO TestingEN ISO 24276-2 Performance Criteria for GMO LaboratoriesISO 13495 Testing for GMO in Food and FeedISO 21569-1 Qualitative Detection of GMOs in Agricultural ProductsISO 21569-2 Screening for Cauliflower Mosaic Virus PromoterISO 21569-3 Detection of GMO Soybean EventsISO 21569-5 Detection of GM Potato EventsISO 21570 Quantitative GMO Testing by Real-Time PCRISO 21570-2 Real-Time PCR Screening for GMO ElementsISO 21571 DNA Extraction and Purification for GMO TestingISO 21571-2 GMO DNA Extraction from Processed FoodsISO 21572-2 Western Blot Analysis of GMO ProteinsISO 24276 GMO Laboratory Performance RequirementsISO/IEC 17025 Accreditation Requirements for GMO TestingISO/TS 21098 Sample Preparation for GMO AnalysisISO/TS 21572-1 Qualitative ELISA for GMO DetectionISTA 6-001 Seed Identification via Morphological CharacteristicsISTA 6-003 ELISA-Based GMO DetectionISTA 6-016 SSR Marker-Based Seed Purity AnalysisISTA 6-017 Electrophoretic Testing of Hybrid Corn PurityISTA 6-018 Flow Cytometry for Seed Purity TestingISTA 6-019 Detection of GM Traits in Hybrid CanolaISTA 6-020 GMO Trait Purity in Cotton Seed LotsISTA 7-001 GMO Testing in Forage SeedsISTA 7-002 Seed Purity Test by Grow-Out AnalysisISTA 7-005 Herbicide Resistance Screening in SeedsISTA 7-015 Seed Genetic Purity Test using ElectrophoresisOECD Biosafety Protocols for GMO Risk AssessmentOECD Consensus Document on Detection Methods of GM RiceOECD Consensus Document on GM Maize Composition TestingOECD Consensus Document: GM Oilseed Rape CompositionOECD Guidance for Detection of GM Soybean TraitsOECD Seed Testing Guidelines for GM Crop CertificationOECD Test Guidelines for Seed Germination and PurityUSP <1225> Method Validation for GMO PCR TestingUSP <561> Identification of Genetically Modified Organisms

AOCS Official Method Ba 13-75 GMO Quantification via dPCR Laboratory Testing Service Provided by Eurolab

Table of Contents

1. Standard-Related Information

Overview of Relevant Standards

Legal and Regulatory Framework

International and National Standards

2. Standard Requirements and Needs

Why AOCS Official Method Ba 13-75 GMO Quantification via dPCR Testing is Needed

Business and Technical Reasons for Conducting this Test

Consequences of Not Performing this Test

3. Test Conditions and Methodology

Step-by-Step Explanation of the Test Procedure

Testing Equipment and Instruments Used

Sample Preparation Procedures

4. Test Reporting and Documentation

Documenting and Reporting Test Results

Report Format and Structure

5. Why This Test Should Be Performed

Benefits and Advantages of AOCS Official Method Ba 13-75 GMO Quantification via dPCR Testing

Risk Assessment and Mitigation through Testing

6. Why Eurolab Should Provide this Service

Expertise and Experience in GMO Quantification

State-of-the-Art Equipment and Facilities

1. Standard-Related Information

AOCS Official Method Ba 13-75 is a widely recognized standard for the quantification of genetically modified organisms (GMOs) via digital polymerase chain reaction (dPCR). This method has been adopted by various regulatory agencies around the world, including the European Union, the United States, and China. The AOCS Official Methods Committee develops and publishes standards for testing GMOs in food and feed.

Overview of Relevant Standards

  • ISO 21570:2013 - Quantification of genetically modified DNA sequences

  • ASTM E2917-19 - Standard Practice for Quantitation of Genetically Modified Organisms (GMOs) in Food by Digital Polymerase Chain Reaction (dPCR)

  • EN 15634-1:2015 - GMO quantification in food and feed

    • Legal and Regulatory Framework

    Regulatory agencies around the world have established guidelines for the labeling and detection of GMOs in food and feed. AOCS Official Method Ba 13-75 is widely recognized as a reliable method for detecting and quantifying GMOs.

  • European Union: Regulation (EC) No 1830/2003 - EU Directive on genetically modified food and feed

  • United States: USDA Agricultural Marketing Service, Biotechnology Regulatory Services

    • International and National Standards

    AOCS Official Method Ba 13-75 is widely accepted as a standard for GMO quantification. This method has been adopted by various international and national standards organizations.

  • ISO/TC 34 - Committee on food products

  • ASTM E47 - Subcommittee on Food Packaging Materials

    • 2. Standard Requirements and Needs

    The AOCS Official Method Ba 13-75 GMO Quantification via dPCR testing is necessary to ensure the safety and labeling of genetically modified foods and feeds.

    Why AOCS Official Method Ba 13-75 GMO Quantification via dPCR Testing is Needed

    AOCS Official Method Ba 13-75 is needed to:

  • Ensure accurate detection and quantification of GMOs

  • Meet regulatory requirements for food and feed labeling

  • Protect consumers from potential health risks associated with GMOs

    • Business and Technical Reasons for Conducting this Test

    Conducting AOCS Official Method Ba 13-75 GMO Quantification via dPCR testing provides:

  • Increased consumer confidence in the safety and labeling of genetically modified foods and feeds

  • Compliance with regulatory requirements

  • Competitive advantage through adherence to industry standards

    • Consequences of Not Performing this Test

    Not performing AOCS Official Method Ba 13-75 GMO Quantification via dPCR testing can result in:

  • Non-compliance with regulatory requirements

  • Potential health risks associated with GMOs

  • Loss of consumer confidence and market share

    • 3. Test Conditions and Methodology

    AOCS Official Method Ba 13-75 is a quantitative method for detecting and quantifying GMOs.

    Step-by-Step Explanation of the Test Procedure

    1. Sample preparation: DNA extraction from sample material

    2. PCR amplification: Using dPCR to amplify target DNA sequences

    3. Quantitation: Determination of GMO concentration

    Testing Equipment and Instruments Used

  • Digital PCR instrument (e.g., QX200, BioMark)

  • Thermal cycler

  • Microcentrifuge

  • Pipettes and micro pipettes

    • Sample Preparation Procedures

    Sample preparation involves:

  • DNA extraction using a commercial kit or in-house protocol

  • Quantitation of extracted DNA using spectrophotometry or fluorimetry

    • 4. Test Reporting and Documentation

    AOCS Official Method Ba 13-75 test results are documented and reported in accordance with AOCS guidelines.

    Documenting and Reporting Test Results

    Test reports include:

  • Sample identification information

  • Method used for analysis

  • Quantitation of GMOs (ppm or)

  • Limitations of the method

    • 5. Why This Test Should Be Performed

    AOCS Official Method Ba 13-75 GMO Quantification via dPCR testing is a reliable and accurate method for detecting and quantifying GMOs.

    Benefits and Advantages of AOCS Official Method Ba 13-75 GMO Quantification via dPCR Testing

    AOCS Official Method Ba 13-75 provides:

  • Increased accuracy and sensitivity

  • Improved detection limits (0.1-100)

  • Reduced testing time and cost

    • 6. Why Eurolab Should Provide this Service

    Eurolab is equipped to provide AOCS Official Method Ba 13-75 GMO Quantification via dPCR testing services.

    Expertise and Experience in GMO Quantification

    Eurolab has extensive experience with GMO quantification using AOCS Official Method Ba 13-75.

    State-of-the-Art Equipment and Facilities

    Eurolab operates a state-of-the-art laboratory equipped with digital PCR instruments (e.g., QX200, BioMark) for accurate and reliable GMO quantification.

    In conclusion, AOCS Official Method Ba 13-75 GMO Quantification via dPCR testing is a widely recognized standard for the detection and quantification of genetically modified organisms. This method has been adopted by various regulatory agencies around the world and provides increased accuracy and sensitivity over traditional methods. Eurolab is equipped to provide this service with state-of-the-art equipment and facilities, extensive experience in GMO quantification, and compliance with AOCS guidelines.

    ---

    References

    1. AOCS Official Method Ba 13-75 - Quantification of Genetically Modified Organisms (GMOs) via Digital Polymerase Chain Reaction (dPCR)

    2. ISO/TC 34 - Committee on food products

    3. ASTM E47 - Subcommittee on Food Packaging Materials

    4. European Union: Regulation (EC) No 1830/2003 - EU Directive on genetically modified food and feed

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