EUROLAB
en-iso-21569-6-digital-pcr-detection-of-gm-maize
GMO & Seed Purity Testing AFNOR NF V03-010 Interpretation of GMO PCR ResultsAFNOR XP V03-020 GMO Analytical Detection Method ValidationAFNOR XP V03-021 Reference Materials for GMO TestingAOAC 2006.01 Roundup Ready Canola GMO QuantificationAOAC 2008.04 GMO Testing for Bt CornAOAC 2013.01 Qualitative DNA Detection for GM CanolaAOAC 2016.04 GMO Screening in Corn and Soy ProductsAOCS Ba 10-65 Impurity Content in Seed SamplesAOCS Ba 11-65 Foreign Matter in Oilseed SamplesAOCS Ba 8-75 Mechanical Purity Determination in SeedsAOCS Ba 9-58 Seed Purity Testing in OilseedsAOCS Official Method Ba 12-75 Detection of GMO in LinseedAOCS Official Method Ba 13-75 GMO Quantification via dPCRCEN/TS 16707:2015 Qualitative Detection of GM CottonEN 13791 GMO Quantification Method for Maize Bt11EN 13835 GMO Detection – Roundup Ready SoyEN 13837 GMO – Event-Specific Detection for MON810EN 16707 Detection of Specific GMO Events in Soya and MaizeEN 16707-1 Detection of GMO Events in FlaxseedEN ISO 21569-4 Real-Time PCR Event Specific GMO DetectionEN ISO 21571-3 GMO DNA Extraction from Oil-Rich SeedsEN ISO 21572 Protein-Based Methods for GMO DetectionEN ISO 21573 Guidelines for GMO Testing LaboratoriesEN ISO 24276 General Laboratory Requirements for GMO TestingEN ISO 24276-2 Performance Criteria for GMO LaboratoriesISO 13495 Testing for GMO in Food and FeedISO 21569-1 Qualitative Detection of GMOs in Agricultural ProductsISO 21569-2 Screening for Cauliflower Mosaic Virus PromoterISO 21569-3 Detection of GMO Soybean EventsISO 21569-5 Detection of GM Potato EventsISO 21570 Quantitative GMO Testing by Real-Time PCRISO 21570-2 Real-Time PCR Screening for GMO ElementsISO 21571 DNA Extraction and Purification for GMO TestingISO 21571-2 GMO DNA Extraction from Processed FoodsISO 21572-2 Western Blot Analysis of GMO ProteinsISO 24276 GMO Laboratory Performance RequirementsISO/IEC 17025 Accreditation Requirements for GMO TestingISO/TS 21098 Sample Preparation for GMO AnalysisISO/TS 21572-1 Qualitative ELISA for GMO DetectionISTA 6-001 Seed Identification via Morphological CharacteristicsISTA 6-003 ELISA-Based GMO DetectionISTA 6-016 SSR Marker-Based Seed Purity AnalysisISTA 6-017 Electrophoretic Testing of Hybrid Corn PurityISTA 6-018 Flow Cytometry for Seed Purity TestingISTA 6-019 Detection of GM Traits in Hybrid CanolaISTA 6-020 GMO Trait Purity in Cotton Seed LotsISTA 7-001 GMO Testing in Forage SeedsISTA 7-002 Seed Purity Test by Grow-Out AnalysisISTA 7-005 Herbicide Resistance Screening in SeedsISTA 7-015 Seed Genetic Purity Test using ElectrophoresisOECD Biosafety Protocols for GMO Risk AssessmentOECD Consensus Document on Detection Methods of GM RiceOECD Consensus Document on GM Maize Composition TestingOECD Consensus Document: GM Oilseed Rape CompositionOECD Guidance for Detection of GM Soybean TraitsOECD Seed Testing Guidelines for GM Crop CertificationOECD Test Guidelines for Seed Germination and PurityUSP <1225> Method Validation for GMO PCR TestingUSP <561> Identification of Genetically Modified Organisms

Comprehensive Guide to EN ISO 21569-6 Digital PCR Detection of GM Maize Testing Services

Provided by Eurolab

EN ISO 21569-6 Digital PCR Detection of GM Maize is a laboratory testing service that involves the detection and quantification of genetically modified (GM) maize DNA using digital polymerase chain reaction (dPCR). This test is essential for ensuring compliance with regulatory requirements, maintaining product safety, and building customer confidence. In this section, we will delve into the relevant standards governing EN ISO 21569-6 Digital PCR Detection of GM Maize testing.

International Standards

  • ISO 21569-6: This standard specifies the requirements for dPCR detection of GM maize.

  • EN ISO 21569-6: This European standard is based on the ISO 21569-6 and provides additional specific requirements for the European market.

    • National Standards

  • In the United States, the relevant standards are:

    • ASTM E2911-14: Standard Practice for Detection of Genetically Modified Organisms (GMOs) in Maize

    US FDA Guidance on Voluntary Labeling Indicating Whether Foods Have or Have Not Been Altered through Genetic Engineering

  • In Canada, the relevant standards are:

    • CAN/CGSB-32.31-2013: Standard for Genetically Modified Organisms (GMOs) in Maize

    Health Canada Guidance on Labelling of Prepackaged Foods Containing Genetically Modified Ingredients

    Standard Development Organizations and Their Role

  • International Organization for Standardization (ISO)

  • European Committee for Standardization (CEN)

  • American Society for Testing and Materials (ASTM)

    • Evolution of Standards

    Standards evolve through a continuous cycle of review, revision, and publication. This ensures that they remain relevant, effective, and aligned with the latest technological advancements.

    Standard Numbers and Scope

    Standard Number Title Scope

    --- --- ---

    ISO 21569-6 Digital PCR detection of genetically modified (GM) maize Specifies requirements for dPCR detection of GM maize DNA.

    EN ISO 21569-6 Digital PCR detection of genetically modified (GM) maize Provides additional specific requirements for the European market.

    Standard Compliance Requirements

    Compliance with relevant standards is essential for industries such as:

  • Food and feed manufacturers

  • Biotechnology companies

  • Agricultural suppliers

    • Non-compliance can result in regulatory fines, product recalls, and damage to reputation.

    EN ISO 21569-6 Digital PCR Detection of GM Maize testing involves the following steps:

    1. Sample Preparation: Maize DNA is extracted from the sample using a validated method.

    2. Digital PCR Amplification: dPCR amplifies the extracted DNA, allowing for sensitive detection and quantification of GM maize DNA.

    3. Data Analysis: The amplified signals are analyzed to determine the presence or absence of GM maize DNA.

    Testing Equipment and Instruments

  • Digital PCR instrument (e.g., BioMark HD)

  • Thermal cycler

  • Microcentrifuge

    • Testing Environment Requirements

  • Temperature: 20-25C

  • Humidity: 30-50

  • Pressure: Atmospheric pressure

    • Sample Preparation Procedures

  • DNA extraction using a validated method

  • Concentration and purification of extracted DNA

    • Testing Parameters and Conditions

  • Sample size: 10-20 mg

  • Template concentration: 1-10 ng/μL

  • Primer concentrations: optimized for dPCR amplification

    • Measurement and Analysis Methods

  • Real-time PCR (qPCR) data analysis software

  • Data validation using quality control samples

    • Calibration and Validation Procedures

  • Regular calibration of the digital PCR instrument

  • Validation of the testing method through regular proficiency testing

    • Quality Control Measures during Testing

  • Use of certified reference materials (CRMs)

  • Regular participation in proficiency testing schemes (PTS)

    • Data Collection and Recording Procedures

  • Electronic data collection using a validated software tool

  • Accurate recording of test results, including any deviations or anomalies

    • Testing Timeframes and Duration

  • Typical testing time: 24-48 hours

  • Turnaround time: 3-5 working days

    • Sample Size Requirements and Statistical Considerations

  • Sample size calculations based on statistical principles (e.g., 95 confidence level)

  • Use of validated statistical software for data analysis

    • The test report includes:

    1. Introduction: Brief overview of the testing service

    2. Testing Methodology: Detailed description of the testing procedure

    3. Results: Tabulated or graphical presentation of test results

    4. Conclusion: Summary of findings and recommendations

    Interpretation of Test Results

  • Presence or absence of GM maize DNA detected

  • Quantification of GM maize DNA concentration (if applicable)

    • Customer Communication

  • Clear communication of testing results, including any deviations or anomalies

  • Provision of certified reference materials for customer verification

    • Why Choose Eurolab for EN ISO 21569-6 Digital PCR Detection of GM Maize Testing Services?

    At Eurolab, we offer:

    1. Experienced laboratory staff: Trained in the latest testing methodologies and standards.

    2. State-of-the-art equipment: Regularly calibrated and maintained to ensure accurate results.

    3. Compliance with regulatory requirements: Expertise in ensuring compliance with relevant national and international standards.

    4. Prompt turnaround times: Flexible scheduling to meet your testing needs.

    Contact us today to learn more about our EN ISO 21569-6 Digital PCR Detection of GM Maize testing services!

    Need help or have a question?
    Contact us for prompt assistance and solutions.

    CONTACT US +902127020000

    Latest News

    View all

    Eurolab Successfully Renews ISO 17025 Accreditation

    Read More

    Expanded Cosmetic Safety Testing Portfolio Now Available at Eurolab

    Read More

    Eurolab Launches Technical Training Series for Industry Professionals

    Read More

    JOIN US
    Want to make a difference?

    Careers