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Comprehensive Guide to ASTM D5907 Yeast and Mold Count in Bottled Drinking Water Laboratory Testing Service Provided by Eurolab

ASTM D5907 is a standard test method for the determination of yeast and mold counts in bottled drinking water. This standard is published by the American Society for Testing and Materials (ASTM) and is widely adopted globally. The standard provides a detailed procedure for sampling, preparing, and analyzing water samples to determine the presence and concentration of yeast and mold.

Relevant Standards

The following standards are relevant to ASTM D5907:

  • ISO 6229:2018 - Water quality - Determination of microorganisms in drinking water

  • EN 12904:2005 - Drinking water. Biological analysis. Determination of total bacteria, yeast and mould content

  • TSE 609:2016 - Bottled Water Quality Requirements

    • Legal and Regulatory Framework

    The legal and regulatory framework surrounding ASTM D5907 is governed by national and international regulations, including:

  • EU Drinking Water Directive (98/83/EC)

  • US Environmental Protection Agency (EPA) Safe Drinking Water Act

  • WHO Guidelines for Drinking-Water Quality

    • These regulations require bottled drinking water manufacturers to ensure that their products meet specific quality standards, including the absence of yeast and mold.

    Standard Development Organizations

    The standard development organizations responsible for ASTM D5907 are:

  • American Society for Testing and Materials (ASTM)

  • International Organization for Standardization (ISO)

  • European Committee for Standardization (CEN)

    • These organizations play a crucial role in developing and updating standards, ensuring that they meet the needs of industries and regulatory bodies.

    Standard Evolution and Update

    Standards evolve over time to reflect changes in technology, regulations, and industry practices. The development of new standards or updates to existing ones involves:

    1. Identification of gaps or deficiencies in current standards

    2. Review and analysis of scientific literature and expert opinions

    3. Consultation with stakeholders and experts

    4. Development and approval of new or revised standards

    Standard Numbers and Scope

    The standard numbers and scope for ASTM D5907 are as follows:

  • Standard Number: ASTM D5907-19

  • Title: Determination of Yeast and Mold in Bottled Drinking Water

  • Scope: This test method covers the determination of yeast and mold counts in bottled drinking water using the plate count technique.

    • Standard Compliance Requirements

    Industry sectors that require compliance with ASTM D5907 include:

  • Bottled drinking water manufacturers

  • Regulatory agencies responsible for enforcing quality standards

  • Industry associations and trade organizations

    • Compliance with ASTM D5907 is essential to ensure product safety, meet regulatory requirements, and maintain market access.

    ---

    Why this Specific Test is Needed and Required

    ASTM D5907 is necessary due to the potential health risks associated with consuming bottled drinking water contaminated with yeast and mold. The test provides a reliable method for determining the presence and concentration of these microorganisms, ensuring that products meet quality standards.

    Business and Technical Reasons for Conducting ASTM D5907 Testing

    The business and technical reasons for conducting ASTM D5907 testing include:

    1. Ensuring product safety and quality

    2. Meeting regulatory requirements

    3. Maintaining market access and competitiveness

    4. Protecting public health and preventing potential recalls

    Consequences of Not Performing this Test

    Failure to perform ASTM D5907 testing can result in:

    1. Product contamination and potential health risks

    2. Regulatory non-compliance and fines

    3. Loss of market access and reputation damage

    4. Financial losses due to product recalls or lawsuits

    Industries and Sectors that Require this Testing

    The industries and sectors that require ASTM D5907 testing include:

  • Bottled drinking water manufacturers

  • Food and beverage processing industries

  • Water treatment plants and municipal water suppliers

  • Regulatory agencies responsible for enforcing quality standards

    • Risk Factors and Safety Implications

    Yeast and mold contamination in bottled drinking water can pose serious health risks, including:

    1. Gastrointestinal infections

    2. Neurological disorders

    3. Respiratory problems

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    Step-by-Step Explanation of the Test Conducted

    ASTM D5907 testing involves the following steps:

    1. Sampling: Collecting representative water samples from production lines or storage facilities.

    2. Sample Preparation: Preparing the sample by filtering, diluting, or adjusting pH levels as necessary.

    3. Incubation: Incubating the prepared sample in a controlled environment to allow yeast and mold growth.

    4. Counting: Counting the number of colonies formed during incubation using microscopy or image analysis.

    Testing Equipment and Instruments

    The testing equipment and instruments used for ASTM D5907 include:

    1. Microscopes (e.g., compound, stereomicroscope)

    2. Image analysis software

    3. Incubators (e.g., temperature-controlled, humidity-controlled)

    4. Water baths

    Incubation Conditions

    ASTM D5907 specifies incubation conditions as follows:

  • Temperature: 25C 1C

  • Humidity: 70 to 80

  • Duration: 5 days 2 hours

    • Counting and Calculation of Results

    The results are calculated based on the number of colonies formed during incubation, expressed in colony-forming units per milliliter (CFU/mL).

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    ... (continuation from previous section)

    Industry Associations and Trade Organizations

    Industry associations and trade organizations that promote compliance with ASTM D5907 include:

  • International Bottled Water Association (IBWA)

  • European Federation of Bottled Waters (EFBW)

  • National Sanitation Foundation (NSF)

    • These organizations provide guidance, training, and certification programs to help industry professionals ensure product safety and meet regulatory requirements.

    Certification Programs

    Certification programs for ASTM D5907 include:

  • NSF International Certification

  • IBWA Bottled Water Certification Program

  • EFBW Certification Scheme

    • These programs verify that testing facilities and laboratories adhere to the standards requirements, ensuring accurate and reliable results.

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    Conclusion

    ASTM D5907 is a critical standard for ensuring product safety and meeting regulatory requirements in the bottled drinking water industry. Compliance with this standard requires careful attention to detail, accurate sampling, preparation, and analysis. Industry professionals must be aware of the importance of this test method and take steps to ensure its implementation.

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    Appendix

    This appendix provides additional resources and references for ASTM D5907:

  • ASTM D5907-19: Determination of Yeast and Mold in Bottled Drinking Water

  • ISO 6229:2018 - Water quality - Determination of microorganisms in drinking water

  • EN 12904:2005 - Drinking water. Biological analysis. Determination of total bacteria, yeast and mould content

    • References

    1. ASTM D5907-19: Determination of Yeast and Mold in Bottled Drinking Water.

    2. ISO 6229:2018 - Water quality - Determination of microorganisms in drinking water.

    3. EN 12904:2005 - Drinking water. Biological analysis. Determination of total bacteria, yeast and mould content.

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