EUROLAB
epa-1624-enteric-virus-detection-in-contaminated-water
Water Microbiology Testing ASTM D3941 Anaerobic Bacteria Detection in Well WaterASTM D3975 Detection of Microbial Contamination in Waterborne PaintsASTM D4016 Microbiological Evaluation of Hydrotherapy PoolsASTM D4025 Impact of Disinfectants on Microbial Water QualityASTM D4516 Presumptive Test for Coliforms in WaterASTM D4517 Heterotrophic Bacteria Enumeration by Spread PlateASTM D5127 Microbial Testing of Deionized and Ultrapure WaterASTM D5187 Sulfate-Reducing Bacteria Enumeration in Cooling SystemsASTM D5243 Microbial Corrosion-Related Organisms in PipelinesASTM D5392 Anaerobic Microbial Detection in Drinking WaterASTM D5464 Bacterial Contamination in High-Purity WaterASTM D5465 Fecal Coliform Detection in Natural WatersASTM D5907 Yeast and Mold Count in Bottled Drinking WaterASTM D6189 Rapid Bacteria Detection in Water Using ATP BioluminescenceASTM D6310 Detection of Microbial-Induced Corrosion in WaterASTM D6311 Aerobic Bacteria Testing in Industrial WaterASTM D6451 Testing Microbial Fouling Potential in WaterASTM D6515 Bioindicator Organism Testing in Water QualityASTM D6596 Microbiological Examination of Cooling WaterASTM D7225 Microbial Growth in Building Water SystemsASTM D7503 Legionella Testing in Potable and Process WaterEN 12780 Microbial Load in Industrial Water for Sterile ProcessesEN 14945 Water Quality – Bacteria Detection Using Flow CytometryEN 1500 Hand Hygiene Microbial Evaluation in Water-Related ActivitiesEN ISO 13843 Validation of Microbial Detection Systems for WaterEN ISO 19458 Sampling Techniques for Microbiological Water TestingEN ISO 20743 Antimicrobial Activity Testing of Water-Exposed TextilesEN ISO 5667-3 Water Sample Preservation for MicrobiologyEPA 1600 Enterococci Detection Using Membrane FiltrationEPA 1601 Male-Specific Coliphage Testing in WaterEPA 1602 Male-specific Bacteriophage Detection in WaterEPA 1603 E. coli Quantification in Surface WaterEPA 1604 Coliform Bacteria Membrane Filtration TechniqueEPA 1605 Aeromonas Testing in Ground and Surface WaterEPA 1606 Bacteroides qPCR Assay for Source Tracking in WaterEPA 1607 Salmonella Quantification in Contaminated WaterEPA 1610 Norovirus Detection in Drinking Water SamplesEPA 1611 Enterococci Detection Using Quantitative PCREPA 1620 Microbial Water Quality Criteria for Drinking WaterEPA 1621 Rapid Pathogen Detection in Recycled WaterEPA 1622 Cryptosporidium Detection Using Filtration MethodEPA 1623 Giardia and Cryptosporidium Detection in Water SamplesEPA 1656 Detection of Protozoan Parasites in WaterEPA 180.1 Turbidity Impact on Microbial Quality AssessmentEPA 200.8 Heavy Metals Impact on Microbiological Water SafetyEPA 524.2 Volatile Microbial Compounds Analysis in Drinking WaterEPA 524.3 Detection of Microbial VOCs in WaterEPA 600 Detection of Opportunistic Pathogens in Building WaterEPA 821-R Pathogen Testing in Treated Industrial WastewaterEPA 9060 Total Organic Carbon Impact on Microbial Water QualityEPA 9065 Pathogen Recovery from Water FiltersEPA 9132 Fecal Indicator Bacteria in Wastewater EffluentsEPA 9215 Total Bacterial Count Using Plate Count MethodISO 10523 Microbial Effects on pH in Water Quality TestingISO 10705-1 Bacteriophage Testing as Viral Indicators in WaterISO 11731 Legionella Testing in Cooling Tower WaterISO 11731-2 Legionella Detection in Biofilm SamplesISO 13136 STEC E. coli Detection in GroundwaterISO 13143 Rapid Screening Method for Pathogens in WaterISO 13145 Rapid Enumeration of E. coli in Fresh WaterISO 13271 Detection of Microsporidia in WaterISO 13799 Detection of Thermophilic Bacteria in Hot Water SystemsISO 13969 Total Coliform Testing in Mineral WaterISO 14189 Detection of Clostridium perfringens in WaterISO 14461 Testing of Spoilage Microorganisms in Bottled WaterISO 14476 Testing of Viral Reduction in Water Disinfection SystemsISO 16266 Pseudomonas aeruginosa Testing in Bottled WaterISO 17381 On-Site Testing of Microbiological Parameters in WaterISO 17994 Comparison Method for Microbial Testing Results in WaterISO 17994 Method Comparison for Drinking Water MicrobiologyISO 17995 Pathogenic Vibrio Detection in Marine and FreshwaterISO 19250 Salmonella Detection in Drinking and Recreational WatersISO 19458 Sampling Strategy for Microbiological Water AnalysisISO 5663 Water Sampling for Microbiological AnalysisISO 6222 Aerobic Colony Count at 22°C and 37°C in WaterISO 6222 Total Viable Bacterial Count in Drinking Water TestingISO 6887-1 Preparation of Water Samples for Microbiological TestingISO 7704 Membrane Filters for Microbiological AnalysisISO 7899-2 Enterococci Detection in Recreational WaterISO 8199 General Guidance for Microbiological Examination of WaterISO 9308-1 E. coli and Coliform Bacteria Detection in WaterISO 9308-2 Rapid Detection of Coliforms Using Chromogenic MediaSM 9210 Bacterial Activity in Water Distribution NetworksSM 9215 Heterotrophic Plate Count in Treated WaterSM 9221 Multiple-Tube Fermentation for Coliform TestingSM 9222 Membrane Filter Technique for Fecal Coliform DetectionSM 9223 Coliform and E. coli Detection Using Colilert MethodSM 9225 Anaerobic Sulfide-Producing Bacteria TestingSM 9226 Thermophilic Bacteria in Thermal Water SourcesSM 9230 Enumeration of Iron and Sulfur Bacteria in WaterSM 9231 Detection of Slime-Forming Bacteria in Distribution SystemsSM 9232 Total Viable Count in Ultrapure WaterSM 9235 Waterborne Pathogen Risk AssessmentSM 9240 Presence/Absence Test for Drinking Water MicrobiologySM 9250 Bacterial Regrowth in Distribution SystemsSM 9260 Detection of Sulfate-Reducing Bacteria in Water SystemsSM 9261 Enumeration of Anaerobes in Sludge and WaterSM 9265 Anaerobic Bacteria in Water and Sludge SamplesSM 9270 Biofilm Bacterial Count in Piping SystemsSM 9280 Testing for Non-Tuberculous Mycobacteria in WaterSM 9285 Fecal Streptococcus Detection in Treated Water

EPA 1624 Enteric Virus Detection in Contaminated Water Laboratory Testing Service: A Comprehensive Guide

The detection of enteric viruses in contaminated water is a critical aspect of ensuring public health safety. The US Environmental Protection Agency (EPA) has established Method 1624, also known as the Enteric Virus Concentration by Filtration and Real-Time Polymerase Chain Reaction method, to detect and quantify enteric viruses in drinking water.

Legal and Regulatory Framework

The Safe Drinking Water Act (SDWA) of 1974 requires the EPA to establish standards for safe drinking water. The SDWA sets maximum contaminant levels (MCLs) for various contaminants, including bacteria, viruses, and other microorganisms. Method 1624 is one of the methods used to detect and quantify enteric viruses in drinking water.

International and National Standards

Method 1624 is based on international standards such as:

  • ISO 13829:2017, Water quality - Sampling of raw water

  • ASTM D7031-17, Standard Practice for In-Situ Testing of Drilled Shafts

  • EN 1487:2002, Water quality - Determination of biological parameters

    • The EPA has adopted these international standards and has also developed its own national standards for enteric virus detection in contaminated water. The relevant US standards include:

  • EPA Method 1624

  • EPA Method 1690

  • ASTM D7069-12

    • Standard Development Organizations

    Standards development organizations (SDOs) play a critical role in developing and maintaining international and national standards. Some of the key SDOs involved in standardization related to water quality include:

  • International Organization for Standardization (ISO)

  • American Society for Testing and Materials (ASTM)

  • European Committee for Standardization (CEN)

    • Standard Evolution and Update

    Standards evolve over time due to advances in technology, changes in regulatory requirements, or the emergence of new contaminants. The standard development process involves:

    1. Research and development

    2. Proposal submission

    3. Review and approval

    4. Publication

    5. Maintenance and update

    Standard Numbers and Scope

    The following are some of the relevant standard numbers and their scope:

  • EPA Method 1624: Enteric Virus Concentration by Filtration and Real-Time Polymerase Chain Reaction

  • ISO 13829:2017: Water quality - Sampling of raw water

  • ASTM D7031-17: Standard Practice for In-Situ Testing of Drilled Shafts

    • Industry Compliance Requirements

    Different industries have varying compliance requirements related to enteric virus detection in contaminated water. For example:

  • Drinking water utilities must comply with EPA Method 1624

  • Industrial facilities may need to comply with ASTM D7031-17 or ISO 13829:2017

    • The detection of enteric viruses in contaminated water is essential for public health safety. This section explains why this specific test is needed and required.

    Business and Technical Reasons

    Enteric virus detection in contaminated water is necessary due to:

    1. Public health risks: Enteric viruses can cause waterborne diseases such as gastroenteritis.

    2. Regulatory requirements: The EPA has established standards for safe drinking water, which include enteric virus detection.

    3. Industry compliance: Various industries have varying compliance requirements related to enteric virus detection.

    Consequences of Not Performing the Test

    Not performing enteric virus detection in contaminated water can lead to:

    1. Public health risks

    2. Regulatory non-compliance

    3. Economic losses due to water treatment and remediation costs

    Industries and Sectors that Require this Testing

    The following industries and sectors require enteric virus detection in contaminated water:

  • Drinking water utilities

  • Industrial facilities

  • Agriculture

  • Municipalities

    • Risk Factors and Safety Implications

    Enteric viruses can cause waterborne diseases, which can lead to:

    1. Gastroenteritis

    2. Diarrhea

    3. Vomiting

    4. Abdominal cramps

    Quality Assurance and Quality Control Aspects

    To ensure accurate results, laboratories must follow quality assurance and control procedures, including:

    1. Standard operating procedures (SOPs)

    2. Calibration and validation of equipment

    3. Sample preparation and testing protocols

    4. Data recording and reporting

    This section provides a detailed step-by-step explanation of how the test is conducted.

    Step 1: Sampling

  • Collect water samples from the source or distribution system.

  • Use approved sampling equipment and procedures.

    • Step 2: Sample Preparation

  • Prepare the sample for analysis using methods such as filtration, centrifugation, or homogenization.

    • Step 3: Testing

  • Conduct real-time polymerase chain reaction (RT-PCR) to detect and quantify enteric viruses.

  • Use approved testing equipment and protocols.

    • Step 4: Data Recording and Reporting

  • Record all data related to the test, including sample information, testing conditions, and results.

  • Report the results in accordance with EPA Method 1624 guidelines.

    • Perspective from a Laboratory Expert

    As a laboratory expert, I can attest that enteric virus detection in contaminated water requires careful attention to detail and adherence to established protocols. Laboratories must follow quality assurance and control procedures to ensure accurate results.

    Test Conditions and Parameters

    The following are some of the critical test conditions and parameters for enteric virus detection:

  • Temperature: 20-25C

  • pH: 6-8

  • Salinity: 10

  • Contaminant levels: 100 CFU/L

    • Equipment Used in Testing

    The following equipment is commonly used for enteric virus detection:

  • RT-PCR machines (e.g., ABI, BioRad)

  • PCR reagents (e.g., primers, probes)

  • Sampling equipment (e.g., bottles, tubing)

    • Common Challenges and Limitations

    Laboratories may encounter challenges such as:

    1. Sample contamination

    2. Equipment malfunction

    3. Data interpretation errors

    Future Directions in Research and Development

    Research and development are ongoing to improve the accuracy and efficiency of enteric virus detection methods. Some areas of focus include:

    1. Developing more sensitive and specific detection assays.

    2. Improving sample preparation protocols for better recovery rates.

    Test Conclusion

    Enteric virus detection in contaminated water is a critical aspect of ensuring public health safety. Laboratories must follow established protocols and adhere to quality assurance and control procedures to ensure accurate results.

    References

  • EPA Method 1624: Enteric Virus Concentration by Filtration and Real-Time Polymerase Chain Reaction

  • ISO 13829:2017, Water quality - Sampling of raw water

  • ASTM D7031-17, Standard Practice for In-Situ Testing of Drilled Shafts

    • Conclusion

    The detection of enteric viruses in contaminated water is essential for public health safety. Laboratories must follow established protocols and adhere to quality assurance and control procedures to ensure accurate results.

    Need help or have a question?
    Contact us for prompt assistance and solutions.

    CONTACT US +902127020000

    Latest News

    View all

    Eurolab Successfully Renews ISO 17025 Accreditation

    Read More

    Expanded Cosmetic Safety Testing Portfolio Now Available at Eurolab

    Read More

    Eurolab Launches Technical Training Series for Industry Professionals

    Read More

    JOIN US
    Want to make a difference?

    Careers