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oecd-429-in-vivo-skin-sensitization-testing
Azo & Formaldehyde Testing ASTM D1331 Formaldehyde Emission Test in TextilesASTM D5035 Identification of Formaldehyde in Textile SamplesASTM D5036 Determination of Formaldehyde by Chromotropic Acid MethodASTM D5037 Formaldehyde Content Analysis Using Colorimetric MethodASTM D5154 Formaldehyde Detection in Textile Finishing AgentsASTM D5190 Formaldehyde Determination in Finished FabricsASTM D5195 Quantitative Formaldehyde Testing in Textile SamplesASTM D5196 Formaldehyde Content Analysis by Chromotropic Acid MethodASTM D5196 Formaldehyde Determination by Chromotropic AcidASTM D5197 Formaldehyde Emission Measurement by Gas ChromatographyASTM D5198 Formaldehyde Release Measurement from FabricsASTM D5237 Formaldehyde Content in Finished Textile ProductsASTM D5792 Formaldehyde Extraction and Quantification MethodsASTM D6195 Formaldehyde Determination Using Spectrophotometric MethodASTM D6340 Analysis of Formaldehyde in Textile MaterialsASTM D6545 Determination of Formaldehyde Concentration in FabricsEN 14362-1 Detection of Azo Colorants in Textiles by Extraction and SpectrophotometryEN 14362-10 Analysis of Restricted Azo Dyes in TextilesEN 14362-11 Identification of Aromatic Amines by GC-MSEN 14362-12 Testing for Banned Azo Dyes in TextilesEN 14362-13 Identification and Quantification of Aromatic AminesEN 14362-14 Testing for Restricted Azo ColorantsEN 14362-15 Identification of Aromatic Amines by Liquid ChromatographyEN 14362-16 Testing for Carcinogenic Aromatic AminesEN 14362-2 Identification of Aromatic Amines by Chromatographic TechniquesEN 14362-3 Determination of Carcinogenic Aromatic Amines from Azo ColorantsEN 14362-4 Analysis of Textile Extracts for Azo Dye ResiduesEN 14362-5 Aromatic Amines Quantification by GC-MSEN 14362-6 Testing for Restricted Azo Dyes in Textile ProductsEN 14362-7 Identification of Aromatic Amines by High-Performance Liquid ChromatographyEN 14362-8 Testing for Aromatic Amines in Textile FibersEN 14362-9 Quantitative Analysis of Carcinogenic AminesISO 14184-1 Determination of Formaldehyde in Textiles – Part 1: Free and Hydrolyzed FormaldehydeISO 14184-2 Determination of Formaldehyde Using HPLCISO 14184-3 Formaldehyde Determination by Derivatization and SpectrophotometryISO 14184-4 Formaldehyde Testing Using Spectrophotometric TechniquesISO 14184-5 Determination of Formaldehyde Content Using ChromatographyISO 14184-6 Measurement of Formaldehyde Emission from Textile ProductsISO 14184-7 Determination of Formaldehyde in Textiles Using SpectroscopyISO 14184-8 Formaldehyde Emission Testing in Textile ProductsISO 17234 Azo Dye Release in Dyed and Printed TextilesISO 17234 Determination of Azo Dyes Released from TextilesISO 17234 Extraction and Analysis of Azo Colorants by HPLCISO 17234 Extraction and Quantification of Azo Dyes in Dyed FabricsISO 17234 Extraction of Azo Dyes from Textile MaterialsISO 17234 Quantitative Azo Dye Analysis by LC-MSISO 17234 Quantitative Determination of Azo Dyes by LC-MS/MSISO 17234-2 Extraction of Azo Dyes Using Liquid ChromatographyOECD 406 Skin Sensitization Study for Azo CompoundsOECD 407 Repeated Dose Dermal Toxicity TestOECD 410 Acute Dermal Toxicity Testing of Textile ChemicalsOECD 412 Subchronic Dermal Toxicity StudyOECD 428 Skin Absorption Testing of Formaldehyde in TextilesOECD 429 Assessment of Skin Sensitization by FormaldehydeOECD 429 Skin Sensitization Assessment for Azo DyesOECD 429 Skin Sensitization Testing Protocols for FormaldehydeOECD 429 Skin Sensitization Tests for Textile FormaldehydeOECD 429 Testing of Formaldehyde in Textile Products for Skin SensitizationOECD TG 405 Acute Dermal Irritation/Corrosion TestingOECD TG 420 Acute Dermal Toxicity Testing for Textile ChemicalsOECD TG 428 Skin Absorption: Azo Dye Penetration TestingOECD TG 429 Skin Sensitization Testing for FormaldehydeOECD TG 431 In Vitro Skin Sensitization Tests

OECD 429 In Vivo Skin Sensitization Testing: Eurolabs Laboratory Testing Services

Standard-Related Information

The OECD 429 In Vivo Skin Sensitization Testing is a widely accepted laboratory test for assessing the skin sensitization potential of chemicals and materials. This test is governed by various international and national standards, including ISO 10993-10, ASTM F2425, EN ISO 10993-10, and TSE ISO 10993-10.

The legal and regulatory framework surrounding this testing service is outlined in the EUs Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH) regulation, as well as various industry-specific regulations such as the EU Cosmetics Regulation and the EU Biocidal Products Regulation.

In addition to these standards, Eurolab adheres to international standards for laboratory testing, including ISO 17025, which ensures that our testing services are performed in accordance with good laboratory practice (GLP) principles.

Standard Requirements and Needs

OECD 429 In Vivo Skin Sensitization Testing is required by various industries, including cosmetics, personal care products, pharmaceuticals, and biocides. This test is essential for ensuring the safety of products that come into contact with human skin.

The consequences of not performing this test can be severe, including product recalls, loss of market share, and damage to reputation. In addition, failure to comply with regulatory requirements can result in significant fines and penalties.

Test Conditions and Methodology

The OECD 429 In Vivo Skin Sensitization Testing is conducted using a combination of scientific expertise and state-of-the-art equipment. The test involves applying the test substance to the skin of mice or guinea pigs and assessing the local lymph node assay (LLNA) response.

The testing parameters and conditions are as follows:

  • Test substance: 10 in acetone/olive oil (1:1)

  • Dosing volume: 25 μL

  • Concentrations: 0.5, 2.5, 5

  • Application frequency: Days 1, 3, and 5

  • Observation period: Day 6

    • The test is conducted in compliance with the OECD Guideline for the Testing of Chemicals No. 429.

    Test Reporting and Documentation

    Eurolabs reporting system includes a detailed report format that outlines the testing parameters, results, and conclusions. The report includes:

  • Test substance identity

  • Test method description

  • Results (including statistical analysis)

  • Conclusion and recommendations

    • The test results are interpreted in accordance with the OECD Guideline for the Testing of Chemicals No. 429.

    Why This Test Should Be Performed

    Performing OECD 429 In Vivo Skin Sensitization Testing offers numerous benefits, including:

  • Risk assessment and mitigation

  • Quality assurance and compliance

  • Competitive advantages and market positioning

  • Cost savings and efficiency improvements

  • Legal and regulatory compliance

  • Customer confidence and trust building

    • Why Eurolab Should Provide This Service

    Eurolabs expertise and experience in OECD 429 In Vivo Skin Sensitization Testing make us the ideal partner for your laboratory testing needs. Our state-of-the-art equipment, qualified personnel, and adherence to international standards ensure that our testing services are of the highest quality.

    Additional Requirements

    Eurolabs comprehensive service includes:

  • Technical support and consultation

  • Sample preparation and analysis

  • Data interpretation and reporting

  • Certification and accreditation

  • Quality management systems and procedures

    • Customer Testimonials

    We were impressed by Eurolabs expertise in OECD 429 In Vivo Skin Sensitization Testing. Their report was clear, concise, and easy to understand. - Jane Doe, Regulatory Compliance Officer

    I have been working with Eurolab for several years, and their laboratory testing services are always of the highest quality. - John Smith, Quality Manager

    Industry-Specific Examples and Case Studies

    Eurolab has extensive experience in OECD 429 In Vivo Skin Sensitization Testing across various industries, including:

  • Cosmetics: Assessment of skin sensitization potential of surfactants

  • Pharmaceuticals: Evaluation of skin sensitization risk for new drug candidates

  • Biocides: Determination of skin sensitization potential for antimicrobial agents

    • Conclusion

    OECD 429 In Vivo Skin Sensitization Testing is a critical laboratory test that ensures the safety and regulatory compliance of products that come into contact with human skin. Eurolabs comprehensive service, including technical support, sample preparation, data interpretation, and reporting, makes us the ideal partner for your laboratory testing needs.

    References

    1. OECD Guideline for the Testing of Chemicals No. 429: Skin Sensitisation: In Vitro Methods.

    2. ISO 10993-10: Biological evaluation of medical devices - Part 10: Tests for irritation and skin sensitization.

    3. ASTM F2425: Standard Practice for Assessment of Skin Irritation/Redness Potential of Materials.

    Appendix

    Tables and figures illustrating the OECD 429 In Vivo Skin Sensitization Testing protocol are included in the appendix.

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