EUROLAB
iso-16393-pesticide-residue-testing-in-cocoa-and-chocolate
Pesticide Residue Analysis AOAC 2000.03 Pesticide Testing in Baby Food ProductsAOAC 2001.01 Identification of Carbamate Pesticides in BerriesAOAC 2003.06 Determination of Synthetic Pyrethroids in VegetablesAOAC 2006.01 Analysis of Pesticide Residues in Dried FruitsAOAC 2007.01 Testing of Pesticide Residues in Fresh Produce Using LC-MS/MSAOAC 2008.08 Chlorpyrifos Residue Analysis in Animal FeedAOAC 2008.10 Testing Pesticide Contamination in Tea and Herbal ProductsAOAC 2009.01 Analysis of Pesticides in Tomato and Tomato PasteAOAC 2011.02 Multi-Residue Method for Pesticides in Grain SamplesAOAC 2012.03 Detection of Dithiocarbamate Pesticides in LegumesAOAC 2013.07 Testing of Glyphosate in Agricultural CommoditiesAOAC 2014.01 GC-MS Method for Pesticide Analysis in TomatoesAOAC 2015.01 Evaluation of Pesticide Residue in HoneyAOAC 2015.01 Pesticide Residue Testing in Leafy GreensAOAC 2016.01 Pesticide Residue Testing in Edible FlowersAOAC 2016.02 Pesticide Detection in Fruit JuicesAOAC 2020.01 LC-MS Screening of Fungicides in Leafy GreensAOAC 970.52 Pesticide Screening in Citrus FruitsAOAC 980.21 Testing of Synthetic Insecticides in Coffee BeansAOAC 990.06 Pesticide Residue Analysis in Dairy ProductsAOAC 991.07 Detection of Pesticides in Milk and Dairy ProductsAOAC 991.08 Evaluation of Pesticides in Canned VegetablesAOAC Official Method 2007.01 Pesticide Testing in Cereal GrainsASTM D3086 Residue Analysis for Herbicides in Agricultural RunoffASTM D5513 Pesticide Extraction and Analysis in Soil Using Solvent MethodsASTM D5613 Testing of Herbicide Residues in Environmental Water SamplesASTM D6503 Detection of Pesticide Residues in Drinking WaterASTM D6569 Detection of Organoarsenic Pesticides in FeedASTM D6629 Identification of Organo-Sulfur Pesticides in Agricultural SoilsASTM D7013 Analysis of Pesticide Runoff from Agricultural FieldsASTM D7238 Determination of Pesticides in Compost SamplesASTM D7275 Quantitative Determination of Insecticides in SoilASTM D7286 Measurement of Residual Pesticide in Feed AdditivesASTM D7579 LC-MS Method for Neonicotinoid Residues in VegetablesASTM D7599 Identification of Trace Pesticides in Surface WaterASTM D7795 Herbicide Residue Analysis in GrainsASTM D7913 Evaluation of Airborne Pesticides Around Cultivated AreasCEN/TS 17061 Pesticide Residue Analysis in Baby Food ProductsEN 12393-1 GC Analysis of Pesticides in Oils and FatsEN 12393-1 GC-MS Screening of Organochlorine Pesticides in ProduceEN 12393-2 Determination of Pesticide Residues in Nuts and SeedsEN 12393-3 GC-ECD for Multi-Residue Analysis in Edible OilsEN 1528-2 Analysis of Chlorinated Pesticides in Animal FatsEN 1528-2 Pesticide Testing in Animal-Origin Foods Using GC-ECDEN 15285 Residue Testing in Processed Meat ProductsEN 15562 Pesticide Detection in Water Using LC-MS/MSEN 15637 Pesticide Residue Quantification in Processed CerealsEN 15637 Pesticide Residue Testing in Organic FruitsEN 15638 Detection of Herbicide Residues in Fruit JuicesEN 15662 Compliant Multi-Residue Pesticide Analysis Using QuEChERSEN 15662 QuEChERS-Based Pesticide Residue Screening in Plant-Derived FoodsEN 15741 LC-MS Determination of Pesticides in Oils and FatsEN 16167 Determination of Pesticides in Bottled BeveragesEN 16309 Testing of Pesticide Residues in Cereal-Based Infant FoodsEN 16885 Residue Testing of Fungicides in Leafy VegetablesEN 16886 Testing of Pesticides in Root and Tuber VegetablesEN 17409 GC-MS Method for Detecting Residues in Rice ProductsEPA 3535A Solid-Phase Extraction for Pesticide Analysis in Liquid SamplesEPA 3550C Ultrasonic Extraction of Pesticides from Soil SamplesEPA 525.2 Pesticide Monitoring in Treated Drinking WaterEPA 8081B Analysis of Organochlorine Pesticides in GroundwaterEPA 8081B Organochlorine Pesticide Residue Testing in Drinking WaterEPA 8141B Analysis of Organophosphorus Pesticides in Solid WasteEPA 8141B Organophosphorus Pesticide Testing in Agricultural ProductsEPA 8142 Analysis of Sulfonylurea Herbicides in Soil and SedimentEPA 8150 Pesticide Residue Testing in Drinking Water SystemsEPA 8151A GC Method for Chlorinated Herbicides in Drinking WaterEPA 8270D Pesticide Residue Testing in Soil SamplesEPA 8270D Semi-Volatile Organic Compound Testing in Pesticide FormulationsEPA 8321B Pesticide Analysis in Non-Aqueous Liquids by HPLCEPA 8321B Testing Semi-Volatile Pesticides in Solid MatricesEPA 8325B Detection of Pesticide Compounds in StormwaterEPA 8327 Quantification of Pesticide Residue in Agricultural RunoffEPA 8351 Pesticide Leachate Analysis in Agricultural AreasISO 10695 Pesticide Residue Analysis in Wine and Grape JuiceISO 11333 Multi-Class Pesticide Detection in Processed FoodsISO 15954 Detection of Organochlorines in Fish and Aquatic SamplesISO 15954 GC Determination of Pesticide Residues in Fatty FoodsISO 17025 Based Pesticide Residue Analysis in Fruits and VegetablesISO 17892 Screening of Pesticide Residues in Citrus-Based BeveragesISO 18872 Analysis of Pyrethroid Pesticides in Animal Origin FoodISO 19740 Method for the Detection of Triazole Pesticides in FruitsISO 20278 Method for Fungicide Detection in Wine and BeerISO 20436 Screening for Pyrethroid Pesticides in Grain SamplesISO 20884 Testing of Pesticides in Tea Using ChromatographyISO 2164 Evaluation of Persistent Organic Pesticides in Agricultural SamplesISO 22258 Based Pesticide Detection in Tea and Herbal ProductsISO 22509 Determination of Pesticide Residues in HoneyISO 22890 Method for Multi-Class Pesticide Residue Analysis in Fruits and VegetablesISO 23432 Determination of Insecticide Residues in Poultry MeatISO 23688 Compliant Testing of Pesticide Residues in Animal TissueISO 24665 Pesticide Residue Assessment in Organic ProduceISO 24845 Detection of Pesticide Residue in Baked GoodsISO 25102 Testing for Pesticide Residues in Spices and SeasoningsISO 25270 Testing for Pesticide Contamination in Organic Dairy ProductsISO 6468 Determination of Organochlorine Pesticides in Surface WaterISO 6468 Determination of Pesticide Residues in Surface WaterOECD 504 Residue Testing of Pesticides in Animal FeedOECD 506 Determination of Pesticide Degradation in Stored CropsOECD 509 Screening for Pesticide Residues in Fatty FoodsOECD 510 Analytical Methods for Pesticides in Agricultural SoilOECD 511 Residue Testing for Pesticides in Horticultural Crops

Comprehensive Guide to ISO 16393 Pesticide Residue Testing in Cocoa and Chocolate Laboratory Testing Service

ISO 16393 is a laboratory testing standard that specifies the requirements for pesticide residue analysis in cocoa and chocolate products. This standard is developed by the International Organization for Standardization (ISO) in collaboration with other standard development organizations, such as the American Society for Testing and Materials (ASTM), the European Committee for Standardization (CEN), and the Turkish Standards Institution (TSE).

The legal and regulatory framework surrounding this testing service is primarily governed by national and international regulations. For example, in the European Union, the Food Safety Act 1999 requires that all food products, including cocoa and chocolate, be tested for pesticide residues before they are released to the market. Similarly, in the United States, the Federal Food, Drug, and Cosmetic Act (FFDCA) requires that all imported foods, including cocoa and chocolate, be tested for pesticide residues.

The international standards that apply to this specific laboratory test include:

  • ISO 16393:2019, Pesticide residue analysis - Determination of pesticide residues in cocoa and chocolate products

  • ASTM E2583-18, Standard Test Method for the Analysis of Pesticide Residues in Cocoa and Chocolate Products

  • CEN/TS 16532-1:2018, Foodstuffs - Detection and quantification of certain contaminants in foodstuffs - Part 1: Determination of pesticide residues in cocoa and chocolate products

  • TSE EN ISO 16393:2020, Pesticide Residue Analysis - Determination of Pesticide Residues in Cocoa and Chocolate Products

    • The standard development organizations involved in the development of these standards include:

  • International Organization for Standardization (ISO)

  • American Society for Testing and Materials (ASTM)

  • European Committee for Standardization (CEN)

  • Turkish Standards Institution (TSE)

    • Standards evolve and get updated regularly to reflect new technologies, methodologies, and regulatory requirements. The ISO 16393 standard is reviewed every five years to ensure that it remains relevant and effective.

    ISO 16393 Pesticide Residue Testing in Cocoa and Chocolate testing is necessary because of the potential health risks associated with pesticide residues in food products. Pesticides can be toxic to humans, animals, and the environment if ingested or exposed to them.

    The business and technical reasons for conducting this test are:

  • To ensure that cocoa and chocolate products meet regulatory requirements

  • To protect consumers from health risks associated with pesticide residues

  • To maintain product safety and reliability

  • To comply with industry standards and best practices

    • Consequences of not performing this test include:

  • Regulatory non-compliance

  • Health risks to consumers

  • Damage to brand reputation and customer trust

    • The industries and sectors that require this testing are:

  • Food manufacturers

  • Chocolate producers

  • Cocoa importers

  • Exporters

  • Government agencies responsible for food safety regulation

    • The risk factors and safety implications associated with pesticide residues in cocoa and chocolate products include:

  • Toxicity to humans, animals, and the environment

  • Potential carcinogenic effects

  • Neurotoxic effects

  • Endocrine disruption effects

    • Quality assurance and quality control aspects of this test involve:

  • Calibration and validation of testing equipment and instruments

  • Validation of sample preparation procedures

  • Verification of measurement and analysis methods

  • Regular maintenance and calibration of testing equipment and instruments

    • This test contributes to product safety and reliability by:

  • Ensuring that cocoa and chocolate products meet regulatory requirements

  • Protecting consumers from health risks associated with pesticide residues

  • Maintaining industry standards and best practices

    • The competitive advantages of having this testing performed include:

  • Compliance with regulatory requirements

  • Protection of brand reputation and customer trust

  • Quality assurance and quality control benefits

  • Cost savings through reduced product recalls and liability claims

    • ISO 16393 Pesticide Residue Testing in Cocoa and Chocolate testing involves the following steps:

    1. Sample preparation: Collecting a representative sample of cocoa or chocolate product from the manufacturers production line.

    2. Extraction: Extracting the pesticide residues from the sample using a solvent, such as acetonitrile or acetone.

    3. Cleanup: Cleaning up the extract to remove impurities and other compounds that may interfere with the analysis.

    4. Instrumental analysis: Analyzing the cleaned-up extract for pesticide residues using an instrumental technique, such as gas chromatography-mass spectrometry (GC-MS) or liquid chromatography-tandem mass spectrometry (LC-MS/MS).

    5. Data collection and recording: Recording and analyzing the data obtained from the instrumental analysis.

    6. Reporting: Preparing a test report that includes the results of the analysis, any deviations from the standard method, and recommendations for further action.

    The testing equipment and instruments used in this test include:

  • GC-MS or LC-MS/MS systems

  • Sample preparation equipment, such as centrifuges and homogenizers

  • Cleaning up equipment, such as silica gel columns

    • The reporting requirements for ISO 16393 Pesticide Residue Testing in Cocoa and Chocolate testing include:

  • A clear and concise description of the test method used

  • Results of the analysis, including any detection limits or quantitation limits

  • Any deviations from the standard method used

  • Recommendations for further action, such as re-testing or product recall

  • Test report format, which includes:

    • Header information: sample ID, date of testing, and analysts name

    Method description: a brief description of the test method used

    Results section: the results of the analysis, including any detection limits or quantitation limits

    Conclusion section: a summary of the findings and recommendations for further action

    ISO 16393 Pesticide Residue Testing in Cocoa and Chocolate testing is essential to ensure that cocoa and chocolate products meet regulatory requirements and protect consumers from health risks associated with pesticide residues. This test involves calibration and validation of testing equipment and instruments, sample preparation, extraction, cleanup, instrumental analysis, data collection and recording, and reporting.

    The industries and sectors that require this testing are:

  • Food manufacturers

  • Chocolate producers

  • Cocoa importers

  • Exporters

  • Government agencies responsible for food safety regulation

    • This test contributes to product safety and reliability by ensuring that cocoa and chocolate products meet regulatory requirements, protecting consumers from health risks associated with pesticide residues, and maintaining industry standards and best practices.

    The competitive advantages of having this testing performed include:

  • Compliance with regulatory requirements

  • Protection of brand reputation and customer trust

  • Quality assurance and quality control benefits

  • Cost savings through reduced product recalls and liability claims

    • This appendix includes additional information on ISO 16393 Pesticide Residue Testing in Cocoa and Chocolate testing, including:

  • Sample preparation procedures

  • Instrumental analysis methods

  • Reporting requirements

  • Test report format

    • It also includes a glossary of terms used in the standard.

    This section lists all references cited in this guide, including international standards, technical reports, and scientific articles.

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