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afnor-nf-en-iso-16140-2-rapid-detection-of-foodborne-pathogens
Food Microbiology Testing AFNOR BRD 07/06-09/98 Listeria Testing in Soft CheesesAFNOR V08-059 Thermoduric Bacteria in MilkAOAC 2003.09 Rapid Salmonella Detection using PCRAOAC 2007.06 Pathogen Detection by Real-Time PCRAOAC 966.23 Microbiological Analysis of Marinated VegetablesAOAC 966.23 Spoilage Detection in Bottled SmoothiesAOAC 966.24 Coliform Testing in Bottled JuicesAOAC 966.24 Coliforms in Soft DrinksAOAC 967.25 Total Viable Count in HoneyAOAC 975.55 Testing of Sauces for S. aureusAOAC 978.18 Water Activity Testing for Microbial RiskAOAC 980.31 Bacillus cereus Detection in Cooked RiceAOAC 991.14 Fecal Coliform Testing in Raw ShellfishAOAC 997.02 Total Mold Count in Dehydrated VegetablesCodex CAC/GL 63 Microbial Risk Assessment for Ready MealsCodex CAC/RCP 43 Microbial Testing of Street FoodsCodex Guidelines / ISO Microbial Control in Organic ProduceCodex STAN 074 Microbial Testing of Infant CerealsCodex STAN 297 Microbiological Testing of Canned VegetablesDIN 10192 Mold Growth Analysis in Pastry ProductsDIN EN 6888-1 Staphylococcus aureus Detection in Cream ProductsDIN EN ISO 11290-1 Listeria Detection in Vacuum-Packed SeafoodDIN EN ISO 4833 Total Viable Count in Dried PastaEC 2073/2005 Detection of Pathogens in Catering ServicesEC 2073/2005 Microbial Contamination Screening in Cooked SausagesEC 2073/2005 Microbial Testing of Edible IceEC 2073/2005 Microbial Testing of Ice CubesEC 2073/2005 Microbiological Criteria for Minced MeatEC 2073/2005 Pathogen Screening in School Lunch MealsFDA BAM Chapter 17 Detection of Clostridium botulinum in Canned FoodsFDA BAM Chapter 19 Enterococcus spp. Detection in Fermented SausageFDA BAM Chapter 5 Pathogen Testing in Fresh JuiceFDA BAM Chapter 5 Salmonella Testing in Egg ProductsFDA BAM Chapter 6 Shigella spp. Detection in VegetablesFDA BAM Chapter 9 Vibrio cholerae Testing in SeafoodFDA BAM Chapter 9 Vibrio spp. Detection in SeafoodFDA BAM General Methods Pathogen Testing in Protein BarsISO 10272-1 Campylobacter spp. Detection in PoultryISO 11133 Microbiological Testing of Commercially Sterile FoodsISO 11290-1 Listeria monocytogenes Detection in Ready-to-Eat FoodsISO 11290-1 Listeria Testing in Cold CutsISO 11290-2 Listeria Enumeration in Soft CheesesISO 14698 Microbial Monitoring in Cold Storage RoomsISO 14698-1 Airborne Microbe Monitoring in Production ZonesISO 15213 Anaerobic Sulfite-Reducing Bacteria TestISO 15214 Lactic Acid Bacteria Enumeration in Fermented VegetablesISO 15216-1 Norovirus Detection in BerriesISO 15216-1 Norovirus Detection in LettuceISO 15216-2 Norovirus Screening in OystersISO 16649-2 E. coli Enumeration in Dairy ProductsISO 16649-2 E. coli Testing in Raw SpinachISO 16649-3 E. coli Detection in Fresh HerbsISO 18593 Food Contact Surface Hygiene TestingISO 18593 Hand Swab Testing for Food HandlersISO 18593 Hygiene Check of Retail Display SurfacesISO 18593 Hygiene Evaluation in Vending MachinesISO 18593 Hygiene Monitoring in Food PlantsISO 18593 Hygiene Monitoring of Slicing MachinesISO 18593 Hygiene Verification in Food Packaging FacilitiesISO 18593 Surface Swab Analysis of Food UtensilsISO 18593 Surface Swab Testing in Food FacilitiesISO 18593 Swab Monitoring of Conveyor BeltsISO 18593 Swab Testing in Hospital KitchensISO 20976-1 Challenge Testing for Acidic FoodsISO 20976-1 Microbial Shelf Life Evaluation of Ready MealsISO 21527-1 Mold Detection in ChocolateISO 21527-1 Spoilage Microorganism Testing in Open SandwichesISO 21527-1 Yeast and Mold Count in Bakery ItemsISO 21527-1 Yeast Enumeration in JuicesISO 21527-2 Mold Count in Processed CheeseISO 21527-2 Mold Detection in Dried FruitsISO 21528-2 Enterobacteriaceae Enumeration in Meat ProductsISO 22964 Cronobacter spp. Detection in Infant FormulaISO 4833-1 Bacterial Count in Raw FishISO 4833-1 Microbial Evaluation of Plant-Based Dairy AlternativesISO 4833-1 Microbial Load in SmoothiesISO 4833-1 Microbial Safety Testing in Meal Kit ServicesISO 4833-1 Microbial Screening of Frozen MealsISO 4833-1 Total Aerobic Colony Count in Food SamplesISO 4833-1 Total Microbial Count in TofuISO 4833-1 Total Plate Count in Hard CheeseISO 4833-1 Total Plate Count in Raw MilkISO 4833-1 Total Viable Count in Packaged SaladsISO 4833-1 TVC in Fast Food MealsISO 4833-2 Spoilage Bacteria in Vacuum-Packaged MeatsISO 6222 Microbiological Quality of Bottled WaterISO 6579 / ISO 11290 Detection of Pathogens in Frozen PizzasISO 6579 / ISO 11290 Pathogen Screening in Baby FoodISO 6579-1 Detection of Salmonella spp. in Meat ProductsISO 6579-1 Salmonella Detection in SpicesISO 6579-1 Salmonella Detection in Tomato SauceISO 6888-1 / ISO 4833-1 Microbial Testing in Plant-Based NuggetsISO 6888-1 Staphylococcus aureus Detection in MilkISO 7889 Microbial Profile of YogurtISO 7932 Bacillus cereus Analysis in Rice SamplesISO 7937 Clostridium perfringens Detection in Cooked FoodsISO 9308-1 Coliform Count in Water for Food UseISO TS 13136 E. coli O157:H7 Detection in BeefUSP 51 Preservative Efficacy Testing in Foods

AFNOR NF EN ISO 16140-2 Rapid Detection of Foodborne Pathogens Laboratory Testing Service Provided by Eurolab: A Comprehensive Guide

The AFNOR NF EN ISO 16140-2 standard is a globally recognized protocol for the rapid detection of foodborne pathogens in various types of samples. This standard is developed and maintained by the International Organization for Standardization (ISO), in collaboration with other standard development organizations, including the European Committee for Electrotechnical Standardization (CENELEC) and the French Association for Normalization (AFNOR).

Legal and Regulatory Framework

The AFNOR NF EN ISO 16140-2 standard is governed by various laws and regulations that dictate the requirements for laboratory testing services. These include:

  • EU Regulation No. 1169/2011 on the provision of food information to consumers

  • EU Regulation No. 178/2002 laying down the general principles and requirements of food law

  • FDAs Food Safety Modernization Act (FSMA) in the United States

  • GMPs (Good Manufacturing Practices) for food products

    • International and National Standards

    The AFNOR NF EN ISO 16140-2 standard is based on the following international standards:

  • ISO/TS 22174:2017, Food safety Microbiology of the food chain General requirements and guidance for testing by numerical simulation

  • ISO 15189:2012, Medical laboratories Requirements for quality and competence

  • EN ISO 16140-1:2016, Microbiology of the food chain Requirements for validation of quantitative detection methods (MDM) for protozoa in water

    • Standard Development Organizations

    The development and maintenance of the AFNOR NF EN ISO 16140-2 standard involve various standard development organizations, including:

  • ISO

  • CENELEC

  • AFNOR

  • ASTM International (American Society for Testing and Materials)

  • TSE (Turkish Standards Institution)

    • These organizations collaborate to ensure that the standard remains up-to-date with the latest scientific research and technological advancements.

    Standard Evolution and Updates

    The development of new standards and updates to existing ones is an ongoing process. This ensures that laboratory testing services remain aligned with changing regulatory requirements, advances in technology, and emerging threats to public health.

    Standard Numbers and Scope

    The AFNOR NF EN ISO 16140-2 standard has the following scope:

  • Detection of foodborne pathogens in various types of samples

  • Application of molecular biology techniques for pathogen detection

  • Validation of quantitative detection methods (MDM) for protozoa in water

    • Industry-Specific Standard Compliance Requirements

    Laboratory testing services must comply with industry-specific standards and regulations. For example, in the food industry:

  • FDAs FSMA requires laboratories to adhere to GMPs and ensure the accuracy and reliability of test results

  • EU Regulation No. 1169/2011 mandates that food manufacturers provide accurate information on the presence of allergens and other substances

    • Standard Compliance Requirements for Different Industries

    Laboratory testing services must comply with industry-specific standards and regulations, including:

  • Food industry: GMPs, FDAs FSMA, EU Regulation No. 1169/2011

  • Pharmaceuticals: cGMPs (current Good Manufacturing Practices)

  • Cosmetics: EU Regulation No. 1223/2009 on cosmetic products

    • The AFNOR NF EN ISO 16140-2 standard is necessary due to the increasing demand for rapid detection of foodborne pathogens in various types of samples. This standard addresses the business and technical requirements for laboratory testing services, ensuring that test results are accurate, reliable, and compliant with regulatory requirements.

    Why This Specific Test is Needed and Required

    The AFNOR NF EN ISO 16140-2 standard is necessary due to:

  • Increasing outbreaks of foodborne illnesses worldwide

  • Growing demand for rapid detection methods

  • Need for laboratory testing services to adhere to GMPs and ensure the accuracy and reliability of test results

    • Business and Technical Reasons for Conducting AFNOR NF EN ISO 16140-2 Rapid Detection of Foodborne Pathogens Testing

    Conducting this test is necessary due to:

  • Regulatory requirements: EU Regulation No. 1169/2011, FDAs FSMA

  • Industry-specific standards: GMPs, cGMPs, EU Regulation No. 1223/2009

  • Growing demand for rapid detection methods

    • Consequences of Not Performing This Test

    Not performing this test can lead to:

  • Increased risk of foodborne illnesses

  • Regulatory non-compliance

  • Loss of customer confidence and trust

    • Industries and Sectors that Require This Testing

    Laboratory testing services must adhere to industry-specific standards, including:

  • Food industry: GMPs, FDAs FSMA, EU Regulation No. 1169/2011

  • Pharmaceuticals: cGMPs (current Good Manufacturing Practices)

  • Cosmetics: EU Regulation No. 1223/2009 on cosmetic products

    • Standard Requirements and Needs for Different Industries

    Laboratory testing services must comply with industry-specific standards and regulations, including:

  • Food industry: GMPs, FDAs FSMA, EU Regulation No. 1169/2011

  • Pharmaceuticals: cGMPs (current Good Manufacturing Practices)

  • Cosmetics: EU Regulation No. 1223/2009 on cosmetic products

    • Testing Methods and Techniques

    Laboratory testing services must apply molecular biology techniques for pathogen detection, including:

  • Polymerase Chain Reaction (PCR)

  • Real-Time PCR

  • Next-Generation Sequencing (NGS)

    • Validation of Quantitative Detection Methods (MDM)

    The AFNOR NF EN ISO 16140-2 standard requires laboratories to validate quantitative detection methods (MDM) for protozoa in water.

    Testing Protocols and Procedures

    Laboratory testing services must adhere to established protocols and procedures, including:

  • Sample collection and preparation

  • DNA extraction and PCR amplification

  • Data analysis and interpretation

    • Quality Control and Assurance

    Laboratory testing services must ensure the accuracy and reliability of test results through quality control and assurance measures, including:

  • Regular calibration of equipment

  • Quality control samples

  • Internal audits and proficiency testing

    • Training and Competence

    Laboratory personnel must undergo training and certification programs to ensure their competence in conducting AFNOR NF EN ISO 16140-2 standard compliant laboratory testing services.

    The AFNOR NF EN ISO 16140-2 standard is necessary due to the increasing demand for rapid detection of foodborne pathogens in various types of samples. This standard addresses the business and technical requirements for laboratory testing services, ensuring that test results are accurate, reliable, and compliant with regulatory requirements.

    Why This Specific Standard is Necessary

    The AFNOR NF EN ISO 16140-2 standard is necessary due to:

  • Increasing outbreaks of foodborne illnesses worldwide

  • Growing demand for rapid detection methods

  • Need for laboratory testing services to adhere to GMPs and ensure the accuracy and reliability of test results

    • Business and Technical Reasons for Conducting AFNOR NF EN ISO 16140-2 Rapid Detection of Foodborne Pathogens Testing

    Conducting this test is necessary due to:

  • Regulatory requirements: EU Regulation No. 1169/2011, FDAs FSMA

  • Industry-specific standards: GMPs, cGMPs, EU Regulation No. 1223/2009

  • Growing demand for rapid detection methods

    • Consequences of Not Performing This Test

    Not performing this test can lead to:

  • Increased risk of foodborne illnesses

  • Regulatory non-compliance

  • Loss of customer confidence and trust

    • Testing Methods and Techniques

    Laboratory testing services must apply molecular biology techniques for pathogen detection, including:

  • Polymerase Chain Reaction (PCR)

  • Real-Time PCR

  • Next-Generation Sequencing (NGS)

    • Validation of Quantitative Detection Methods (MDM)

    The AFNOR NF EN ISO 16140-2 standard requires laboratories to validate quantitative detection methods (MDM) for protozoa in water.

    Testing Protocols and Procedures

    Laboratory testing services must adhere to established protocols and procedures, including:

  • Sample collection and preparation

  • DNA extraction and PCR amplification

  • Data analysis and interpretation

    • Quality Control and Assurance

    Laboratory testing services must ensure the accuracy and reliability of test results through quality control and assurance measures, including:

  • Regular calibration of equipment

  • Quality control samples

  • Internal audits and proficiency testing

    • Training and Competence

    Laboratory personnel must undergo training and certification programs to ensure their competence in conducting AFNOR NF EN ISO 16140-2 standard compliant laboratory testing services.

    Conclusion

    The AFNOR NF EN ISO 16140-2 standard is necessary for the rapid detection of foodborne pathogens in various types of samples. This standard addresses the business and technical requirements for laboratory testing services, ensuring that test results are accurate, reliable, and compliant with regulatory requirements.

    Recommendations

    Laboratory testing services must:

  • Adhere to industry-specific standards and regulations

  • Ensure the accuracy and reliability of test results through quality control and assurance measures

  • Provide training and certification programs for laboratory personnel

    • By following this comprehensive guide, laboratory testing services can ensure compliance with the AFNOR NF EN ISO 16140-2 standard and provide accurate, reliable, and compliant results.

    Future Developments

    The development of new standards and updates to existing ones is an ongoing process. This ensures that laboratory testing services remain aligned with changing regulatory requirements, advances in technology, and emerging threats.

    Continuing Education

    Laboratory personnel must undergo continuing education programs to stay up-to-date with the latest developments and advancements in molecular biology techniques for pathogen detection.

    By following this comprehensive guide, laboratory testing services can ensure compliance with the AFNOR NF EN ISO 16140-2 standard and provide accurate, reliable, and compliant results.

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