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iso-ts-13136-e-coli-o157h7-detection-in-beef
Food Microbiology Testing AFNOR BRD 07/06-09/98 Listeria Testing in Soft CheesesAFNOR NF EN ISO 16140-2 Rapid Detection of Foodborne PathogensAFNOR V08-059 Thermoduric Bacteria in MilkAOAC 2003.09 Rapid Salmonella Detection using PCRAOAC 2007.06 Pathogen Detection by Real-Time PCRAOAC 966.23 Microbiological Analysis of Marinated VegetablesAOAC 966.23 Spoilage Detection in Bottled SmoothiesAOAC 966.24 Coliform Testing in Bottled JuicesAOAC 966.24 Coliforms in Soft DrinksAOAC 967.25 Total Viable Count in HoneyAOAC 975.55 Testing of Sauces for S. aureusAOAC 978.18 Water Activity Testing for Microbial RiskAOAC 980.31 Bacillus cereus Detection in Cooked RiceAOAC 991.14 Fecal Coliform Testing in Raw ShellfishAOAC 997.02 Total Mold Count in Dehydrated VegetablesCodex CAC/GL 63 Microbial Risk Assessment for Ready MealsCodex CAC/RCP 43 Microbial Testing of Street FoodsCodex Guidelines / ISO Microbial Control in Organic ProduceCodex STAN 074 Microbial Testing of Infant CerealsCodex STAN 297 Microbiological Testing of Canned VegetablesDIN 10192 Mold Growth Analysis in Pastry ProductsDIN EN 6888-1 Staphylococcus aureus Detection in Cream ProductsDIN EN ISO 11290-1 Listeria Detection in Vacuum-Packed SeafoodDIN EN ISO 4833 Total Viable Count in Dried PastaEC 2073/2005 Detection of Pathogens in Catering ServicesEC 2073/2005 Microbial Contamination Screening in Cooked SausagesEC 2073/2005 Microbial Testing of Edible IceEC 2073/2005 Microbial Testing of Ice CubesEC 2073/2005 Microbiological Criteria for Minced MeatEC 2073/2005 Pathogen Screening in School Lunch MealsFDA BAM Chapter 17 Detection of Clostridium botulinum in Canned FoodsFDA BAM Chapter 19 Enterococcus spp. Detection in Fermented SausageFDA BAM Chapter 5 Pathogen Testing in Fresh JuiceFDA BAM Chapter 5 Salmonella Testing in Egg ProductsFDA BAM Chapter 6 Shigella spp. Detection in VegetablesFDA BAM Chapter 9 Vibrio cholerae Testing in SeafoodFDA BAM Chapter 9 Vibrio spp. Detection in SeafoodFDA BAM General Methods Pathogen Testing in Protein BarsISO 10272-1 Campylobacter spp. Detection in PoultryISO 11133 Microbiological Testing of Commercially Sterile FoodsISO 11290-1 Listeria monocytogenes Detection in Ready-to-Eat FoodsISO 11290-1 Listeria Testing in Cold CutsISO 11290-2 Listeria Enumeration in Soft CheesesISO 14698 Microbial Monitoring in Cold Storage RoomsISO 14698-1 Airborne Microbe Monitoring in Production ZonesISO 15213 Anaerobic Sulfite-Reducing Bacteria TestISO 15214 Lactic Acid Bacteria Enumeration in Fermented VegetablesISO 15216-1 Norovirus Detection in BerriesISO 15216-1 Norovirus Detection in LettuceISO 15216-2 Norovirus Screening in OystersISO 16649-2 E. coli Enumeration in Dairy ProductsISO 16649-2 E. coli Testing in Raw SpinachISO 16649-3 E. coli Detection in Fresh HerbsISO 18593 Food Contact Surface Hygiene TestingISO 18593 Hand Swab Testing for Food HandlersISO 18593 Hygiene Check of Retail Display SurfacesISO 18593 Hygiene Evaluation in Vending MachinesISO 18593 Hygiene Monitoring in Food PlantsISO 18593 Hygiene Monitoring of Slicing MachinesISO 18593 Hygiene Verification in Food Packaging FacilitiesISO 18593 Surface Swab Analysis of Food UtensilsISO 18593 Surface Swab Testing in Food FacilitiesISO 18593 Swab Monitoring of Conveyor BeltsISO 18593 Swab Testing in Hospital KitchensISO 20976-1 Challenge Testing for Acidic FoodsISO 20976-1 Microbial Shelf Life Evaluation of Ready MealsISO 21527-1 Mold Detection in ChocolateISO 21527-1 Spoilage Microorganism Testing in Open SandwichesISO 21527-1 Yeast and Mold Count in Bakery ItemsISO 21527-1 Yeast Enumeration in JuicesISO 21527-2 Mold Count in Processed CheeseISO 21527-2 Mold Detection in Dried FruitsISO 21528-2 Enterobacteriaceae Enumeration in Meat ProductsISO 22964 Cronobacter spp. Detection in Infant FormulaISO 4833-1 Bacterial Count in Raw FishISO 4833-1 Microbial Evaluation of Plant-Based Dairy AlternativesISO 4833-1 Microbial Load in SmoothiesISO 4833-1 Microbial Safety Testing in Meal Kit ServicesISO 4833-1 Microbial Screening of Frozen MealsISO 4833-1 Total Aerobic Colony Count in Food SamplesISO 4833-1 Total Microbial Count in TofuISO 4833-1 Total Plate Count in Hard CheeseISO 4833-1 Total Plate Count in Raw MilkISO 4833-1 Total Viable Count in Packaged SaladsISO 4833-1 TVC in Fast Food MealsISO 4833-2 Spoilage Bacteria in Vacuum-Packaged MeatsISO 6222 Microbiological Quality of Bottled WaterISO 6579 / ISO 11290 Detection of Pathogens in Frozen PizzasISO 6579 / ISO 11290 Pathogen Screening in Baby FoodISO 6579-1 Detection of Salmonella spp. in Meat ProductsISO 6579-1 Salmonella Detection in SpicesISO 6579-1 Salmonella Detection in Tomato SauceISO 6888-1 / ISO 4833-1 Microbial Testing in Plant-Based NuggetsISO 6888-1 Staphylococcus aureus Detection in MilkISO 7889 Microbial Profile of YogurtISO 7932 Bacillus cereus Analysis in Rice SamplesISO 7937 Clostridium perfringens Detection in Cooked FoodsISO 9308-1 Coliform Count in Water for Food UseUSP 51 Preservative Efficacy Testing in Foods

Complete Guide to ISO TS 13136 E. coli O157:H7 Detection in Beef Testing Services Provided by Eurolab

Standard-Related Information

ISO TS 13136 is a technical specification developed by the International Organization for Standardization (ISO) that provides guidelines for the detection of Escherichia coli O157:H7 in beef. This standard is part of a larger series of ISO standards related to microbiological analysis and safety testing.

Legal and Regulatory Framework

The legal and regulatory framework surrounding ISO TS 13136 E. coli O157:H7 Detection in Beef testing includes various national and international regulations that govern the production, processing, and distribution of beef products. These regulations aim to ensure public health and safety by establishing standards for microbiological analysis and testing.

International and National Standards

Several international and national standards apply to ISO TS 13136 E. coli O157:H7 Detection in Beef testing:

  • ISO TS 13136:2014 (Technical Specification) - Detection of Escherichia coli O157:H7 in beef
  • ISO 16140-1:2003 (Standard) - Microbiology of the food chain General requirements and guidelines for microbiological analysis
  • ASTM E2595-05 (Standard) - Standard Guide for Sampling Beef for Microbiological Analysis
  • EN ISO 16654:2014 (Standard) - Foodstuffs Horizontal method for the detection of Escherichia coli O157:H7
  • Standard Development Organizations

    The development and maintenance of standards such as ISO TS 13136 are overseen by standard development organizations, including:

  • International Organization for Standardization (ISO)
  • American Society for Testing and Materials (ASTM)
  • European Committee for Standardization (CEN)
  • Evolution and Update of Standards

    Standards evolve and get updated over time to reflect new scientific knowledge, technological advancements, and changing regulatory requirements. This ensures that testing methods remain accurate, reliable, and relevant.

    Standard Numbers and Scope

    Key standard numbers and their scope include:

  • ISO TS 13136:2014 (Technical Specification) - Detection of Escherichia coli O157:H7 in beef
  • Applies to the detection of E. coli O157:H7 in beef samples using a variety of methods, including PCR and culture-based techniques.

    Compliance Requirements

    Compliance with standards such as ISO TS 13136 is essential for industries involved in beef production, processing, and distribution, as well as laboratories that conduct testing services.

    Industry/ SectorCompliance Requirements
    Beef ProducersMeet regulatory requirements for E. coli O157:H7 testing
    Food ProcessorsEnsure accurate and reliable E. coli O157:H7 detection in beef products
    LaboratoriesConduct testing according to ISO TS 13136 guidelines and standards

    Standard Requirements and Needs

    ISO TS 13136 E. coli O157:H7 Detection in Beef testing is necessary due to the potential risks associated with consuming contaminated beef products. The consequences of not performing this test include:

  • Public health risks: Consuming contaminated beef products can lead to foodborne illnesses, particularly for vulnerable populations such as the elderly and young children.
  • Financial losses: Contaminated beef products can result in product recalls, loss of market share, and financial penalties.
  • Business and Technical Reasons

    Conducting ISO TS 13136 E. coli O157:H7 Detection in Beef testing provides several business and technical benefits:

  • Quality assurance: Ensures the accuracy and reliability of microbiological analysis results.
  • Risk management: Mitigates public health risks associated with consuming contaminated beef products.
  • Compliance: Meets regulatory requirements for E. coli O157:H7 testing.
  • Consequences of Not Performing This Test

    The consequences of not performing this test include:

  • Public health risks: Consuming contaminated beef products can lead to foodborne illnesses, particularly for vulnerable populations such as the elderly and young children.
  • Financial losses: Contaminated beef products can result in product recalls, loss of market share, and financial penalties.
  • Industries and Sectors that Require This Testing

    Beef producers, processors, distributors, and laboratories involved in testing services require ISO TS 13136 E. coli O157:H7 Detection in Beef testing to ensure public health and safety.

    Risk Factors and Safety Implications

    E. coli O157:H7 is a pathogenic bacterium that can cause severe foodborne illnesses. Consuming contaminated beef products poses significant risks to human health, particularly for vulnerable populations such as the elderly and young children.

    Methods of Detection

    ISO TS 13136 provides guidelines for various methods of detection, including:

  • Polymerase Chain Reaction (PCR): A molecular biology technique that amplifies specific DNA sequences.
  • Culture-based techniques: Methods used to isolate and grow E. coli O157:H7 from beef samples.
  • Standard Requirements for Laboratories

    Laboratories conducting ISO TS 13136 E. coli O157:H7 Detection in Beef testing must:

  • Follow standard guidelines and protocols
  • Use accredited equipment and reagents
  • Maintain accurate records of test results
  • Interpretation and Reporting of Results

    Results should be interpreted according to standard guidelines, and reporting should include:

  • Presence/Absence: Indication of whether E. coli O157:H7 is present or absent in the sample.
  • Concentration: Quantification of E. coli O157:H7 in the sample.
  • Sampling and Preparation

    Proper sampling and preparation techniques are essential to ensure accurate results, including:

  • Sample collection: Collection of beef samples from production facilities, processing plants, or distribution centers.
  • Sample handling: Proper storage and transportation of samples to minimize contamination risks.
  • Testing Methods

    Several testing methods can be used for E. coli O157:H7 detection in beef, including:

  • Polymerase Chain Reaction (PCR): A molecular biology technique that amplifies specific DNA sequences.
  • Culture-based techniques: Methods used to isolate and grow E. coli O157:H7 from beef samples.
  • Testing Protocols

    Standard testing protocols must be followed for accurate results, including:

  • Test sample preparation
  • Equipment calibration
  • Reagent verification
  • Sample analysis
  • Quality Control Measures

    Laboratories conducting ISO TS 13136 E. coli O157:H7 Detection in Beef testing should implement quality control measures to ensure accuracy and reliability of results, including:

  • Calibration: Regular calibration of equipment
  • Verification: Verification of reagents and materials
  • Proficiency testing: Participation in proficiency testing programs
  • Reporting and Record-Keeping

    Accurate reporting and record-keeping are essential for laboratories conducting ISO TS 13136 E. coli O157:H7 Detection in Beef testing, including:

  • Test result reports
  • Sample records
  • Equipment maintenance logs
  • The following sections will cover specific aspects of the standard, such as sampling and preparation, testing methods, and quality control measures.

    Sampling and Preparation

    Proper sampling and preparation techniques are essential to ensure accurate results, including:

  • Sample collection: Collection of beef samples from production facilities, processing plants, or distribution centers.
  • Sample handling: Proper storage and transportation of samples to minimize contamination risks.
  • Testing Methods

    Several testing methods can be used for E. coli O157:H7 detection in beef, including:

  • Polymerase Chain Reaction (PCR): A molecular biology technique that amplifies specific DNA sequences.
  • Culture-based techniques: Methods used to isolate and grow E. coli O157:H7 from beef samples.
  • Testing Protocols

    Standard testing protocols must be followed for accurate results, including:

  • Test sample preparation
  • Equipment calibration
  • Reagent verification
  • Sample analysis
  • Quality Control Measures

    Laboratories conducting ISO TS 13136 E. coli O157:H7 Detection in Beef testing should implement quality control measures to ensure accuracy and reliability of results, including:

  • Calibration: Regular calibration of equipment
  • Verification: Verification of reagents and materials
  • Proficiency testing: Participation in proficiency testing programs
  • Reporting and Record-Keeping

    Accurate reporting and record-keeping are essential for laboratories conducting ISO TS 13136 E. coli O157:H7 Detection in Beef testing, including:

  • Test result reports
  • Sample records
  • Equipment maintenance logs
  • Interpretation of Results

    Results should be interpreted according to standard guidelines, and reporting should include:

  • Presence/Absence: Indication of whether E. coli O157:H7 is present or absent in the sample.
  • Concentration: Quantification of E. coli O157:H7 in the sample.
  • Continuing Education and Training

    Laboratory personnel conducting ISO TS 13136 E. coli O157:H7 Detection in Beef testing should receive ongoing education and training to stay up-to-date with standard guidelines, equipment maintenance, and quality control measures.

    Conclusion

    ISO TS 13136 E. coli O157:H7 Detection in Beef testing is an essential component of ensuring public health and safety in the beef industry. Laboratories conducting this testing must adhere to standard guidelines and protocols to ensure accurate results and reliability.

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