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aoac-97555-testing-of-sauces-for-s-aureus
Food Microbiology Testing AFNOR BRD 07/06-09/98 Listeria Testing in Soft CheesesAFNOR NF EN ISO 16140-2 Rapid Detection of Foodborne PathogensAFNOR V08-059 Thermoduric Bacteria in MilkAOAC 2003.09 Rapid Salmonella Detection using PCRAOAC 2007.06 Pathogen Detection by Real-Time PCRAOAC 966.23 Microbiological Analysis of Marinated VegetablesAOAC 966.23 Spoilage Detection in Bottled SmoothiesAOAC 966.24 Coliform Testing in Bottled JuicesAOAC 966.24 Coliforms in Soft DrinksAOAC 967.25 Total Viable Count in HoneyAOAC 978.18 Water Activity Testing for Microbial RiskAOAC 980.31 Bacillus cereus Detection in Cooked RiceAOAC 991.14 Fecal Coliform Testing in Raw ShellfishAOAC 997.02 Total Mold Count in Dehydrated VegetablesCodex CAC/GL 63 Microbial Risk Assessment for Ready MealsCodex CAC/RCP 43 Microbial Testing of Street FoodsCodex Guidelines / ISO Microbial Control in Organic ProduceCodex STAN 074 Microbial Testing of Infant CerealsCodex STAN 297 Microbiological Testing of Canned VegetablesDIN 10192 Mold Growth Analysis in Pastry ProductsDIN EN 6888-1 Staphylococcus aureus Detection in Cream ProductsDIN EN ISO 11290-1 Listeria Detection in Vacuum-Packed SeafoodDIN EN ISO 4833 Total Viable Count in Dried PastaEC 2073/2005 Detection of Pathogens in Catering ServicesEC 2073/2005 Microbial Contamination Screening in Cooked SausagesEC 2073/2005 Microbial Testing of Edible IceEC 2073/2005 Microbial Testing of Ice CubesEC 2073/2005 Microbiological Criteria for Minced MeatEC 2073/2005 Pathogen Screening in School Lunch MealsFDA BAM Chapter 17 Detection of Clostridium botulinum in Canned FoodsFDA BAM Chapter 19 Enterococcus spp. Detection in Fermented SausageFDA BAM Chapter 5 Pathogen Testing in Fresh JuiceFDA BAM Chapter 5 Salmonella Testing in Egg ProductsFDA BAM Chapter 6 Shigella spp. Detection in VegetablesFDA BAM Chapter 9 Vibrio cholerae Testing in SeafoodFDA BAM Chapter 9 Vibrio spp. Detection in SeafoodFDA BAM General Methods Pathogen Testing in Protein BarsISO 10272-1 Campylobacter spp. Detection in PoultryISO 11133 Microbiological Testing of Commercially Sterile FoodsISO 11290-1 Listeria monocytogenes Detection in Ready-to-Eat FoodsISO 11290-1 Listeria Testing in Cold CutsISO 11290-2 Listeria Enumeration in Soft CheesesISO 14698 Microbial Monitoring in Cold Storage RoomsISO 14698-1 Airborne Microbe Monitoring in Production ZonesISO 15213 Anaerobic Sulfite-Reducing Bacteria TestISO 15214 Lactic Acid Bacteria Enumeration in Fermented VegetablesISO 15216-1 Norovirus Detection in BerriesISO 15216-1 Norovirus Detection in LettuceISO 15216-2 Norovirus Screening in OystersISO 16649-2 E. coli Enumeration in Dairy ProductsISO 16649-2 E. coli Testing in Raw SpinachISO 16649-3 E. coli Detection in Fresh HerbsISO 18593 Food Contact Surface Hygiene TestingISO 18593 Hand Swab Testing for Food HandlersISO 18593 Hygiene Check of Retail Display SurfacesISO 18593 Hygiene Evaluation in Vending MachinesISO 18593 Hygiene Monitoring in Food PlantsISO 18593 Hygiene Monitoring of Slicing MachinesISO 18593 Hygiene Verification in Food Packaging FacilitiesISO 18593 Surface Swab Analysis of Food UtensilsISO 18593 Surface Swab Testing in Food FacilitiesISO 18593 Swab Monitoring of Conveyor BeltsISO 18593 Swab Testing in Hospital KitchensISO 20976-1 Challenge Testing for Acidic FoodsISO 20976-1 Microbial Shelf Life Evaluation of Ready MealsISO 21527-1 Mold Detection in ChocolateISO 21527-1 Spoilage Microorganism Testing in Open SandwichesISO 21527-1 Yeast and Mold Count in Bakery ItemsISO 21527-1 Yeast Enumeration in JuicesISO 21527-2 Mold Count in Processed CheeseISO 21527-2 Mold Detection in Dried FruitsISO 21528-2 Enterobacteriaceae Enumeration in Meat ProductsISO 22964 Cronobacter spp. Detection in Infant FormulaISO 4833-1 Bacterial Count in Raw FishISO 4833-1 Microbial Evaluation of Plant-Based Dairy AlternativesISO 4833-1 Microbial Load in SmoothiesISO 4833-1 Microbial Safety Testing in Meal Kit ServicesISO 4833-1 Microbial Screening of Frozen MealsISO 4833-1 Total Aerobic Colony Count in Food SamplesISO 4833-1 Total Microbial Count in TofuISO 4833-1 Total Plate Count in Hard CheeseISO 4833-1 Total Plate Count in Raw MilkISO 4833-1 Total Viable Count in Packaged SaladsISO 4833-1 TVC in Fast Food MealsISO 4833-2 Spoilage Bacteria in Vacuum-Packaged MeatsISO 6222 Microbiological Quality of Bottled WaterISO 6579 / ISO 11290 Detection of Pathogens in Frozen PizzasISO 6579 / ISO 11290 Pathogen Screening in Baby FoodISO 6579-1 Detection of Salmonella spp. in Meat ProductsISO 6579-1 Salmonella Detection in SpicesISO 6579-1 Salmonella Detection in Tomato SauceISO 6888-1 / ISO 4833-1 Microbial Testing in Plant-Based NuggetsISO 6888-1 Staphylococcus aureus Detection in MilkISO 7889 Microbial Profile of YogurtISO 7932 Bacillus cereus Analysis in Rice SamplesISO 7937 Clostridium perfringens Detection in Cooked FoodsISO 9308-1 Coliform Count in Water for Food UseISO TS 13136 E. coli O157:H7 Detection in BeefUSP 51 Preservative Efficacy Testing in Foods

AOAC 975.55 Testing of Sauces for S. aureus: Laboratory Testing Services Provided by Eurolab

The AOAC 975.55 testing method is a widely accepted standard for detecting Staphylococcus aureus in sauces. This method is governed by various international and national standards, including:

  • ISO 11133:2006 - Microbiology of food and animal feeding stuffs - Horizontal method for the detection of specific micro-organisms
  • ASTM E2424-09 - Standard Test Method for Determination of Total Viable Bacterial Count (TVC) in Foods
  • EN 13697:2012 - Microbiology of the food chain - Horizontal method for the enumeration of microorganisms - Colony-count technique at 30C
  • TSE (Turkish Standards Institution) 1333-2005 - Food Safety, Bacteriological Analysis of Food and Beverage
  • The legal and regulatory framework surrounding this testing service is governed by various regulations, including:

  • EU Regulation 178/2002 on the General Principles of Food Law
  • FDAs Good Manufacturing Practice (GMP) Regulations
  • USDAs Hazard Analysis Critical Control Point (HACCP) Regulations
  • International standard development organizations, such as ISO and ASTM, play a crucial role in shaping the standards for AOAC 975.55 testing. These organizations regularly review and update standards to reflect changes in technology, industry practices, and regulatory requirements.

    The scope of AOAC 975.55 testing covers the detection of S. aureus in sauces, which are defined as foods that contain a liquid or semi-liquid component with a high water content. This test is essential for ensuring the safety and quality of sauces, particularly those intended for human consumption.

    Industry-specific compliance requirements for AOAC 975.55 testing vary depending on factors such as product type, production process, and market destination. For example:

  • Food manufacturers: Compliance with food safety regulations, GMPs, and HACCP requirements
  • Beverage manufacturers: Compliance with beverage safety regulations and industry standards
  • Retailers: Compliance with food safety regulations and industry standards
  • The AOAC 975.55 testing method is necessary for several reasons:

    1. Ensuring product safety: S. aureus can cause foodborne illnesses, particularly in sauces that are intended for human consumption.

    2. Preventing economic losses: A single outbreak of S. aureus contamination can result in significant economic losses for manufacturers and retailers.

    3. Maintaining regulatory compliance: Manufacturers must comply with food safety regulations, GMPs, and HACCP requirements to avoid penalties and reputational damage.

    Industries that require AOAC 975.55 testing include:

  • Food manufacturers (e.g., sauces, condiments, and dressings)
  • Beverage manufacturers (e.g., juice, nectar, and syrups)
  • Retailers (e.g., supermarkets, restaurants, and foodservice providers)
  • Risk factors and safety implications associated with S. aureus contamination in sauces include:

  • Foodborne illnesses: S. aureus can cause skin infections, respiratory tract infections, and sepsis.
  • Economic losses: Contamination outbreaks can result in significant economic losses for manufacturers and retailers.
  • Quality assurance and quality control aspects of AOAC 975.55 testing are critical to ensuring accurate and reliable results. These aspects include:

  • Sample preparation procedures
  • Testing equipment calibration and validation
  • Quality control measures during testing
  • Data collection and recording procedures
  • The AOAC 975.55 testing method involves the following steps:

    1. Sample preparation: Sauces are homogenized, diluted, and plated onto selective agar medium.

    2. Incubation: Plates are incubated at 37C for 24-48 hours.

    3. Observation: Colonies are observed and counted using a stereomicroscope or magnifying glass.

    Testing equipment and instruments used include:

  • Incubators
  • Stereomicroscopes or magnifying glasses
  • Pipettes
  • Micropipettes
  • Testing environment requirements include:

  • Temperature: 37C 1C
  • Humidity: 50-60
  • Pressure: Atmospheric pressure
  • Sample preparation procedures involve:

  • Homogenization of sauces
  • Dilution of sauces
  • Plating onto selective agar medium
  • Testing parameters and conditions include:

  • Incubation temperature and time
  • Observation criteria (e.g., colony size, shape, color)
  • Measurement and analysis methods used include:

  • Colony counting using a stereomicroscope or magnifying glass
  • Microbiological analysis software for data processing and interpretation
  • Calibration and validation procedures involve:

  • Regular calibration of testing equipment
  • Validation of testing methods and protocols
  • Quality control measures during testing include:

  • Use of quality control samples (e.g., positive controls, negative controls)
  • Regular monitoring of testing equipment performance
  • Data recording and documentation procedures
  • Conclusion

    AOAC 975.55 testing is a widely accepted standard for detecting S. aureus in sauces. This method is essential for ensuring the safety and quality of sauces, particularly those intended for human consumption. Industry-specific compliance requirements for AOAC 975.55 testing vary depending on factors such as product type, production process, and market destination.

    Laboratory Testing Services Provided by Eurolab

    Eurolab offers comprehensive laboratory testing services for AOAC 975.55 testing, including:

  • Sample preparation
  • Testing equipment calibration and validation
  • Quality control measures during testing
  • Data collection and recording procedures
  • Our experienced team of microbiologists and quality control specialists ensure accurate and reliable results, meeting the highest standards of quality and regulatory compliance.

    Contact Us

    For more information on AOAC 975.55 testing or to schedule a test, please contact us at:

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