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aoac-200706-pathogen-detection-by-real-time-pcr
Food Microbiology Testing AFNOR BRD 07/06-09/98 Listeria Testing in Soft CheesesAFNOR NF EN ISO 16140-2 Rapid Detection of Foodborne PathogensAFNOR V08-059 Thermoduric Bacteria in MilkAOAC 2003.09 Rapid Salmonella Detection using PCRAOAC 966.23 Microbiological Analysis of Marinated VegetablesAOAC 966.23 Spoilage Detection in Bottled SmoothiesAOAC 966.24 Coliform Testing in Bottled JuicesAOAC 966.24 Coliforms in Soft DrinksAOAC 967.25 Total Viable Count in HoneyAOAC 975.55 Testing of Sauces for S. aureusAOAC 978.18 Water Activity Testing for Microbial RiskAOAC 980.31 Bacillus cereus Detection in Cooked RiceAOAC 991.14 Fecal Coliform Testing in Raw ShellfishAOAC 997.02 Total Mold Count in Dehydrated VegetablesCodex CAC/GL 63 Microbial Risk Assessment for Ready MealsCodex CAC/RCP 43 Microbial Testing of Street FoodsCodex Guidelines / ISO Microbial Control in Organic ProduceCodex STAN 074 Microbial Testing of Infant CerealsCodex STAN 297 Microbiological Testing of Canned VegetablesDIN 10192 Mold Growth Analysis in Pastry ProductsDIN EN 6888-1 Staphylococcus aureus Detection in Cream ProductsDIN EN ISO 11290-1 Listeria Detection in Vacuum-Packed SeafoodDIN EN ISO 4833 Total Viable Count in Dried PastaEC 2073/2005 Detection of Pathogens in Catering ServicesEC 2073/2005 Microbial Contamination Screening in Cooked SausagesEC 2073/2005 Microbial Testing of Edible IceEC 2073/2005 Microbial Testing of Ice CubesEC 2073/2005 Microbiological Criteria for Minced MeatEC 2073/2005 Pathogen Screening in School Lunch MealsFDA BAM Chapter 17 Detection of Clostridium botulinum in Canned FoodsFDA BAM Chapter 19 Enterococcus spp. Detection in Fermented SausageFDA BAM Chapter 5 Pathogen Testing in Fresh JuiceFDA BAM Chapter 5 Salmonella Testing in Egg ProductsFDA BAM Chapter 6 Shigella spp. Detection in VegetablesFDA BAM Chapter 9 Vibrio cholerae Testing in SeafoodFDA BAM Chapter 9 Vibrio spp. Detection in SeafoodFDA BAM General Methods Pathogen Testing in Protein BarsISO 10272-1 Campylobacter spp. Detection in PoultryISO 11133 Microbiological Testing of Commercially Sterile FoodsISO 11290-1 Listeria monocytogenes Detection in Ready-to-Eat FoodsISO 11290-1 Listeria Testing in Cold CutsISO 11290-2 Listeria Enumeration in Soft CheesesISO 14698 Microbial Monitoring in Cold Storage RoomsISO 14698-1 Airborne Microbe Monitoring in Production ZonesISO 15213 Anaerobic Sulfite-Reducing Bacteria TestISO 15214 Lactic Acid Bacteria Enumeration in Fermented VegetablesISO 15216-1 Norovirus Detection in BerriesISO 15216-1 Norovirus Detection in LettuceISO 15216-2 Norovirus Screening in OystersISO 16649-2 E. coli Enumeration in Dairy ProductsISO 16649-2 E. coli Testing in Raw SpinachISO 16649-3 E. coli Detection in Fresh HerbsISO 18593 Food Contact Surface Hygiene TestingISO 18593 Hand Swab Testing for Food HandlersISO 18593 Hygiene Check of Retail Display SurfacesISO 18593 Hygiene Evaluation in Vending MachinesISO 18593 Hygiene Monitoring in Food PlantsISO 18593 Hygiene Monitoring of Slicing MachinesISO 18593 Hygiene Verification in Food Packaging FacilitiesISO 18593 Surface Swab Analysis of Food UtensilsISO 18593 Surface Swab Testing in Food FacilitiesISO 18593 Swab Monitoring of Conveyor BeltsISO 18593 Swab Testing in Hospital KitchensISO 20976-1 Challenge Testing for Acidic FoodsISO 20976-1 Microbial Shelf Life Evaluation of Ready MealsISO 21527-1 Mold Detection in ChocolateISO 21527-1 Spoilage Microorganism Testing in Open SandwichesISO 21527-1 Yeast and Mold Count in Bakery ItemsISO 21527-1 Yeast Enumeration in JuicesISO 21527-2 Mold Count in Processed CheeseISO 21527-2 Mold Detection in Dried FruitsISO 21528-2 Enterobacteriaceae Enumeration in Meat ProductsISO 22964 Cronobacter spp. Detection in Infant FormulaISO 4833-1 Bacterial Count in Raw FishISO 4833-1 Microbial Evaluation of Plant-Based Dairy AlternativesISO 4833-1 Microbial Load in SmoothiesISO 4833-1 Microbial Safety Testing in Meal Kit ServicesISO 4833-1 Microbial Screening of Frozen MealsISO 4833-1 Total Aerobic Colony Count in Food SamplesISO 4833-1 Total Microbial Count in TofuISO 4833-1 Total Plate Count in Hard CheeseISO 4833-1 Total Plate Count in Raw MilkISO 4833-1 Total Viable Count in Packaged SaladsISO 4833-1 TVC in Fast Food MealsISO 4833-2 Spoilage Bacteria in Vacuum-Packaged MeatsISO 6222 Microbiological Quality of Bottled WaterISO 6579 / ISO 11290 Detection of Pathogens in Frozen PizzasISO 6579 / ISO 11290 Pathogen Screening in Baby FoodISO 6579-1 Detection of Salmonella spp. in Meat ProductsISO 6579-1 Salmonella Detection in SpicesISO 6579-1 Salmonella Detection in Tomato SauceISO 6888-1 / ISO 4833-1 Microbial Testing in Plant-Based NuggetsISO 6888-1 Staphylococcus aureus Detection in MilkISO 7889 Microbial Profile of YogurtISO 7932 Bacillus cereus Analysis in Rice SamplesISO 7937 Clostridium perfringens Detection in Cooked FoodsISO 9308-1 Coliform Count in Water for Food UseISO TS 13136 E. coli O157:H7 Detection in BeefUSP 51 Preservative Efficacy Testing in Foods

AOAC 2007.06 Pathogen Detection by Real-Time PCR Laboratory Testing Service: A Comprehensive Guide

As a leading laboratory testing service provider, Eurolab offers AOAC 2007.06 Pathogen Detection by Real-Time PCR testing to ensure the safety and reliability of products in various industries. This article provides a detailed guide on the standard-related information, requirements and needs, test conditions and methodology, test reporting and documentation, and why this test should be performed.

The AOAC 2007.06 method for pathogen detection by real-time PCR is based on the international standards for molecular biology techniques. The relevant standards include:

  • ISO/IEC 17025:2018 - General requirements for the competence of testing and calibration laboratories

  • ASTM E2531-17 - Standard Guide for Selecting and Using a Molecular Biological Method to Detect and Quantify Pathogens in Food, Water, or Environmental Samples

  • EN ISO/IEC 17020:2012 - Conformity assessment -- Requirements for the operation of various types of bodies performing inspection

    • The legal and regulatory framework surrounding this testing service is governed by national and international regulations. For example:

  • EU Regulation (EC) No 2073/2005 on Microbiological Criteria for Foodstuffs

  • FDA Guidelines for Industry: Juice HACCP Systems

  • USDA Pathogen Reduction; Hazard Analysis and Critical Control Points (HACCP) Systems

    • The standards that apply to this specific laboratory test include:

  • AOAC 2007.06 - Pathogen Detection by Real-Time PCR Methodology

  • ISO/TS 22005:2016 - Food safety management systems -- Requirements for bodies providing audit and certification of food safety management systems

    • Standard development organizations play a crucial role in maintaining the accuracy and reliability of laboratory testing services. These organizations include:

  • International Organization for Standardization (ISO)

  • American Society for Testing and Materials (ASTM)

  • European Committee for Standardization (CEN)

    • Standards evolve and get updated periodically to reflect changes in technology, methodology, and industry requirements.

    The AOAC 2007.06 method is needed to ensure the detection of pathogens in various food products. This testing service is required by:

  • Food manufacturers to ensure product safety

  • Regulatory agencies to enforce food safety regulations

  • Consumers to ensure safe consumption

    • Consequences of not performing this test include:

  • Product contamination and recalls

  • Loss of consumer trust and reputation damage

  • Non-compliance with regulatory requirements

    • This testing service is essential for various industries, including:

  • Food processing and manufacturing

  • Pharmaceutical and biotechnology

  • Research and development institutions

    • The risk factors and safety implications associated with pathogen detection include:

  • Risk of product contamination and foodborne illnesses

  • Compliance with regulatory requirements

  • Quality assurance and quality control measures to ensure accurate results

    • The AOAC 2007.06 method involves the following steps:

    1. Sample preparation: Collecting and processing samples for PCR analysis.

    2. PCR setup: Preparing reagents, primers, and probes for real-time PCR reaction.

    3. Real-time PCR analysis: Running the PCR reaction on a real-time PCR instrument to detect target DNA sequences.

    4. Data analysis: Interpreting the results using specific software packages.

    The testing equipment and instruments used include:

  • Real-time PCR instrument (e.g., ABI 7500 Fast or similar)

  • Thermocycler

  • Pipettes and other laboratory equipment

    • The testing environment requirements include:

  • Temperature control (22-25C)

  • Humidity control (50-60)

  • Pressure control

  • Cleanroom conditions for sample preparation

    • The test results are documented and reported in a standard format, including:

  • Sample identification and description

  • Methodology used

  • Results of PCR analysis (including DNA concentration, Ct values)

  • Data interpretation and conclusions

    • The report includes certification and accreditation aspects, such as:

  • Accreditation by a recognized accrediting body

  • Compliance with regulatory requirements

  • Reference to relevant standards and guidelines

    • Electronic reporting systems are used to ensure efficient data management and transfer.

    This testing service provides numerous benefits and advantages, including:

  • Risk assessment and mitigation through testing

  • Quality assurance and compliance benefits

  • Competitive advantages and market positioning

  • Cost savings and efficiency improvements

  • Legal and regulatory compliance benefits

  • Customer confidence and trust building

  • International market access and trade facilitation

  • Innovation and research development support

  • Environmental and sustainability considerations

    • Eurolab offers the AOAC 2007.06 method due to its expertise and experience in molecular biology techniques, quality assurance, and regulatory compliance.

    Key benefits of choosing Eurolab for this testing service include:

  • State-of-the-art laboratory equipment and facilities

  • Experienced and trained personnel

  • Compliance with regulatory requirements and industry standards

  • Efficient data management and transfer using electronic reporting systems

    • Eurolabs commitment to providing accurate and reliable results ensures customer satisfaction and trust.

    Conclusion

    The AOAC 2007.06 method for pathogen detection by real-time PCR is an essential testing service that ensures the safety and reliability of products in various industries. This comprehensive guide provides standard-related information, requirements and needs, test conditions and methodology, test reporting and documentation, and why this test should be performed.

    Eurolabs expertise and experience in molecular biology techniques, quality assurance, and regulatory compliance make it an ideal partner for laboratories seeking to provide accurate and reliable results for the AOAC 2007.06 method.

    By choosing Eurolab for this testing service, customers can ensure compliance with regulatory requirements, maintain product safety, and protect their reputation.

    Need help or have a question?
    Contact us for prompt assistance and solutions.

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