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iso-11290-2-listeria-enumeration-in-soft-cheeses
Food Microbiology Testing AFNOR BRD 07/06-09/98 Listeria Testing in Soft CheesesAFNOR NF EN ISO 16140-2 Rapid Detection of Foodborne PathogensAFNOR V08-059 Thermoduric Bacteria in MilkAOAC 2003.09 Rapid Salmonella Detection using PCRAOAC 2007.06 Pathogen Detection by Real-Time PCRAOAC 966.23 Microbiological Analysis of Marinated VegetablesAOAC 966.23 Spoilage Detection in Bottled SmoothiesAOAC 966.24 Coliform Testing in Bottled JuicesAOAC 966.24 Coliforms in Soft DrinksAOAC 967.25 Total Viable Count in HoneyAOAC 975.55 Testing of Sauces for S. aureusAOAC 978.18 Water Activity Testing for Microbial RiskAOAC 980.31 Bacillus cereus Detection in Cooked RiceAOAC 991.14 Fecal Coliform Testing in Raw ShellfishAOAC 997.02 Total Mold Count in Dehydrated VegetablesCodex CAC/GL 63 Microbial Risk Assessment for Ready MealsCodex CAC/RCP 43 Microbial Testing of Street FoodsCodex Guidelines / ISO Microbial Control in Organic ProduceCodex STAN 074 Microbial Testing of Infant CerealsCodex STAN 297 Microbiological Testing of Canned VegetablesDIN 10192 Mold Growth Analysis in Pastry ProductsDIN EN 6888-1 Staphylococcus aureus Detection in Cream ProductsDIN EN ISO 11290-1 Listeria Detection in Vacuum-Packed SeafoodDIN EN ISO 4833 Total Viable Count in Dried PastaEC 2073/2005 Detection of Pathogens in Catering ServicesEC 2073/2005 Microbial Contamination Screening in Cooked SausagesEC 2073/2005 Microbial Testing of Edible IceEC 2073/2005 Microbial Testing of Ice CubesEC 2073/2005 Microbiological Criteria for Minced MeatEC 2073/2005 Pathogen Screening in School Lunch MealsFDA BAM Chapter 17 Detection of Clostridium botulinum in Canned FoodsFDA BAM Chapter 19 Enterococcus spp. Detection in Fermented SausageFDA BAM Chapter 5 Pathogen Testing in Fresh JuiceFDA BAM Chapter 5 Salmonella Testing in Egg ProductsFDA BAM Chapter 6 Shigella spp. Detection in VegetablesFDA BAM Chapter 9 Vibrio cholerae Testing in SeafoodFDA BAM Chapter 9 Vibrio spp. Detection in SeafoodFDA BAM General Methods Pathogen Testing in Protein BarsISO 10272-1 Campylobacter spp. Detection in PoultryISO 11133 Microbiological Testing of Commercially Sterile FoodsISO 11290-1 Listeria monocytogenes Detection in Ready-to-Eat FoodsISO 11290-1 Listeria Testing in Cold CutsISO 14698 Microbial Monitoring in Cold Storage RoomsISO 14698-1 Airborne Microbe Monitoring in Production ZonesISO 15213 Anaerobic Sulfite-Reducing Bacteria TestISO 15214 Lactic Acid Bacteria Enumeration in Fermented VegetablesISO 15216-1 Norovirus Detection in BerriesISO 15216-1 Norovirus Detection in LettuceISO 15216-2 Norovirus Screening in OystersISO 16649-2 E. coli Enumeration in Dairy ProductsISO 16649-2 E. coli Testing in Raw SpinachISO 16649-3 E. coli Detection in Fresh HerbsISO 18593 Food Contact Surface Hygiene TestingISO 18593 Hand Swab Testing for Food HandlersISO 18593 Hygiene Check of Retail Display SurfacesISO 18593 Hygiene Evaluation in Vending MachinesISO 18593 Hygiene Monitoring in Food PlantsISO 18593 Hygiene Monitoring of Slicing MachinesISO 18593 Hygiene Verification in Food Packaging FacilitiesISO 18593 Surface Swab Analysis of Food UtensilsISO 18593 Surface Swab Testing in Food FacilitiesISO 18593 Swab Monitoring of Conveyor BeltsISO 18593 Swab Testing in Hospital KitchensISO 20976-1 Challenge Testing for Acidic FoodsISO 20976-1 Microbial Shelf Life Evaluation of Ready MealsISO 21527-1 Mold Detection in ChocolateISO 21527-1 Spoilage Microorganism Testing in Open SandwichesISO 21527-1 Yeast and Mold Count in Bakery ItemsISO 21527-1 Yeast Enumeration in JuicesISO 21527-2 Mold Count in Processed CheeseISO 21527-2 Mold Detection in Dried FruitsISO 21528-2 Enterobacteriaceae Enumeration in Meat ProductsISO 22964 Cronobacter spp. Detection in Infant FormulaISO 4833-1 Bacterial Count in Raw FishISO 4833-1 Microbial Evaluation of Plant-Based Dairy AlternativesISO 4833-1 Microbial Load in SmoothiesISO 4833-1 Microbial Safety Testing in Meal Kit ServicesISO 4833-1 Microbial Screening of Frozen MealsISO 4833-1 Total Aerobic Colony Count in Food SamplesISO 4833-1 Total Microbial Count in TofuISO 4833-1 Total Plate Count in Hard CheeseISO 4833-1 Total Plate Count in Raw MilkISO 4833-1 Total Viable Count in Packaged SaladsISO 4833-1 TVC in Fast Food MealsISO 4833-2 Spoilage Bacteria in Vacuum-Packaged MeatsISO 6222 Microbiological Quality of Bottled WaterISO 6579 / ISO 11290 Detection of Pathogens in Frozen PizzasISO 6579 / ISO 11290 Pathogen Screening in Baby FoodISO 6579-1 Detection of Salmonella spp. in Meat ProductsISO 6579-1 Salmonella Detection in SpicesISO 6579-1 Salmonella Detection in Tomato SauceISO 6888-1 / ISO 4833-1 Microbial Testing in Plant-Based NuggetsISO 6888-1 Staphylococcus aureus Detection in MilkISO 7889 Microbial Profile of YogurtISO 7932 Bacillus cereus Analysis in Rice SamplesISO 7937 Clostridium perfringens Detection in Cooked FoodsISO 9308-1 Coliform Count in Water for Food UseISO TS 13136 E. coli O157:H7 Detection in BeefUSP 51 Preservative Efficacy Testing in Foods

ISO 11290-2 Listeria Enumeration in Soft Cheeses Laboratory Testing Service: A Comprehensive Guide

Overview of ISO 11290-2 Standard

The International Organization for Standardization (ISO) has developed a comprehensive standard for the enumeration of Listeria monocytogenes in soft cheeses. The standard, titled Microbiology of food and animal feeding stuffs Horizontal method for the detection and enumeration of Listeria monocytogenes, is designated as ISO 11290-2:1998.

Relevant Standards and Regulations

  • ISO 11290-1: This standard provides general guidance on the sampling and preparation of samples for analysis.

  • EN 15789: This European Standard sets out the requirements for the enumeration of Listeria monocytogenes in soft cheeses.

  • TSE (Turkish Standards Institution): TSE standards are used in Turkey to ensure compliance with national regulations.

  • ASTM E2499-11: This American Society for Testing and Materials standard provides guidelines for the detection and enumeration of Listeria monocytogenes.

    • Legal and Regulatory Framework

  • The European Unions (EU) Food Safety Authority (EFSA) has established a framework for food safety, which includes regulations on Listeria monocytogenes in soft cheeses.

  • In the United States, the FDA regulates the presence of Listeria monocytogenes in foods, including soft cheeses.

  • Other countries may have their own national regulations and standards for Listeria monocytogenes detection.

    • Standard Development Organizations

    The development of ISO 11290-2 was led by a committee of experts from various countries. The standard has undergone several revisions to ensure it remains relevant and effective.

    Evolution of Standards

    Standards are regularly updated to reflect new scientific knowledge, technological advancements, or changes in regulatory requirements. Eurolabs laboratory testing services stay up-to-date with the latest standards and regulations.

    Standard Numbers and Scope

  • ISO 11290-2:1998 Horizontal method for the detection and enumeration of Listeria monocytogenes

  • EN 15789:2011 Detection and enumeration of Listeria monocytogenes in soft cheeses

    • Why ISO 11290-2 is Required

    The presence of Listeria monocytogenes in soft cheeses poses a significant risk to consumer health. This test ensures compliance with national regulations, reduces the risk of contamination, and promotes product safety.

    Business and Technical Reasons for Conducting this Test

  • Compliance: Regulatory bodies require regular testing to ensure compliance with food safety standards.

  • Risk Management: Testing helps identify potential risks and prevent contamination.

  • Quality Assurance: Regular testing ensures that products meet quality and safety requirements.

  • Competitive Advantage: Demonstrating a commitment to product safety can enhance market positioning.

    • Consequences of Not Performing this Test

    Failure to detect Listeria monocytogenes in soft cheeses may result in:

  • Consumer health risks

  • Regulatory non-compliance

  • Damage to brand reputation

  • Economic losses due to product recalls or market withdrawal

    • Test Procedure Overview

    The ISO 11290-2 standard outlines a detailed procedure for the detection and enumeration of Listeria monocytogenes in soft cheeses. The test involves:

    1. Sample Collection: Soft cheese samples are collected from production sites or suppliers.

    2. Preparation: Samples are prepared according to the standards guidelines, which includes homogenization, filtration, and inoculation with a selective medium.

    3. Inoculation: Selective agar plates are inoculated with the sample preparation, allowing for the growth of Listeria monocytogenes.

    4. Incubation: Plates are incubated at specific temperatures (30C 1C) to promote the growth of Listeria monocytogenes.

    5. Enumeration: The number of colonies is counted and expressed as CFU/g (colony-forming units per gram).

    Testing Equipment and Instruments

    Eurolabs laboratory uses advanced equipment, such as:

  • Homogenizers

  • Centrifuges

  • Microscopes

  • Inoculation loops

  • Selective agar plates

    • Reporting Requirements

    The test report includes the following information:

    1. Sample Identification: Soft cheese type, production date, and batch number.

    2. Testing Parameters: Testing method, sampling procedure, and analytical techniques used.

    3. Results: Listeria monocytogenes enumeration (CFU/g) and any associated microbiological data.

    4. Conclusion: Interpretation of results in relation to regulatory requirements.

    Documentation Requirements

    All test reports must be accompanied by:

    1. Raw Data: Raw analytical data, including images or photographs of the agar plates.

    2. Sampling Protocol: Detailed description of sampling procedures and protocols used.

    3. Analytical Method: A brief description of the testing method employed.

  • Quality Control: Regular quality control checks to ensure test accuracy and precision.

  • Calibration: Calibration of all testing equipment according to manufacturers guidelines.

  • Training: Ongoing training for laboratory personnel to maintain expertise in Listeria monocytogenes detection.

    • Eurolabs expert team is dedicated to providing reliable, precise, and efficient laboratory testing services.

    Need help or have a question?
    Contact us for prompt assistance and solutions.

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