EUROLAB
en-iso-21528-2-enumeration-of-enterobacteriaceae-in-confectionery
Microbiological Testing AOAC 2000.14 Aerobic Plate Count in Dairy ProductsAOAC 2001.02 Surface Swab Testing in Kitchen EquipmentAOAC 2003.01 Campylobacter Detection in Poultry ProductsAOAC 2003.07 Detection of Campylobacter spp. in Food and Environmental SamplesAOAC 2004.02 Yeast and Mold Count in Fruit JuiceAOAC 2004.05 Mold Count in Nuts and SeedsAOAC 2005.03 Detection of Enterotoxins in Canned VegetablesAOAC 2007.01 Listeria Detection in Processed MeatsAOAC 2008.06 Norovirus Detection in Leafy GreensAOAC 2011.02 E. coli O157:H7 Testing in SproutsAOAC 2011.03 Detection of Cronobacter spp. in Infant FormulaAOAC 2013.09 Vibrio Detection in SeafoodAOAC 2015.01 Enumeration of Thermophilic Spores in MilkAOAC 2016.03 Total Aerobic Bacteria in Raw VegetablesAOAC 2017.09 Yeast Detection in BeveragesAOAC 2020.01 Cronobacter Enumeration in Infant CerealsAOAC 978.18 Standard Plate Count in Dairy ProductsAOAC 978.18 Total Coliform Test in ShellfishAOAC 978.24 Total Coliform Count in Food SamplesAOAC 983.19 Coliforms in Ice Cream ProductsAOAC 986.33 Staphylococcus aureus Count in SandwichesAOAC 990.12 Coagulase-Positive Staphylococci in FoodAOAC 990.12 Lactic Acid Bacteria in Fermented FoodsAOAC 991.14 Bacillus cereus Detection in Cooked RiceAOAC 991.14 Salmonella Detection Using ELISA MethodAOAC 991.15 Detection of Bacillus cereus in FoodsAOAC 996.08 Listeria monocytogenes Screening in Ready-to-Eat FoodsAOAC 996.08 Salmonella Detection in Dried FoodsAOAC 998.08 Detection of Escherichia coli O157:H7 in FoodsAOAC 998.08 Yeast and Mold Count in Fruit JuicesASTM D4783 Rapid Enumeration of Aerobic Microorganisms in Food PackagingASTM E1428 Evaluation of Antifungal Properties on Food Contact SurfacesASTM E2617 Detection of Mycotoxins-Producing Fungi by PCRASTM E2754 Standard Guide for Sampling of Microbial Contamination on SurfacesASTM E2876 Determination of Bacterial Endotoxins in Water Used in Food ProcessingASTM E2881 Rapid Detection of Pathogens in Food Using BiosensorEN 13697 Quantitative Surface Testing for Microbial ReductionEN 13704 Sporocidal Activity Testing of Disinfectants in Food AreasEN 1650 Quantitative Suspension Test for the Evaluation of Fungicidal ActivityEN ISO 10272-1 Detection of Campylobacter in Raw ChickenEN ISO 10272-2 Enumeration of Campylobacter in MeatEN ISO 11290-1 Detection of Listeria in Vacuum-Packaged FishEN ISO 11290-2 Enumeration of Listeria monocytogenes in Soft CheeseEN ISO 11731 Detection of Legionella in Cooling Tower WaterEN ISO 11731 Legionella Detection in Hot Water SystemsEN ISO 11731-2 Legionella Detection in Low Nutrient EnvironmentsEN ISO 13720 Pseudomonas spp. in Refrigerated ProductsEN ISO 13720 Spoilage Bacteria Testing in Vacuum-Packaged MeatEN ISO 14189 Enumeration of Sulfite-Reducing Bacteria in FoodEN ISO 14189 Sulfite-Reducing Anaerobes in Food Water SystemsEN ISO 15213 Enumeration of Anaerobic Bacteria in Canned FoodsEN ISO 15216-1 Detection of Norovirus and HAV in ShellfishEN ISO 15216-2 Quantitative Detection of Norovirus in BerriesEN ISO 16212 Detection of Fungi in Plant-Based FoodEN ISO 16266 Detection of Pseudomonas in Drinking WaterEN ISO 16649 Enumeration of Beta-Glucuronidase-Positive E. coliEN ISO 16649-1 Enumeration of Beta-Glucuronidase-Positive E. coliEN ISO 16654 Detection of E. coli O157 in Leafy GreensEN ISO 17410 Counting of Psychrotrophs in Refrigerated Ready MealsEN ISO 17604 Swab Sampling Techniques in Meat IndustryEN ISO 17604-2 Sampling for Bacterial Analysis in PoultryEN ISO 18465 Detection of Human Norovirus in OystersEN ISO 18465 Norovirus Detection in Frozen ShellfishEN ISO 19036 Statistical Uncertainty in Microbiological ResultsEN ISO 21149 Microbial Limits in Cosmetic Food-Based IngredientsEN ISO 21527-1 Enumeration of Yeast in Acidified Canned ProductsEN ISO 21527-2 Enumeration of Yeasts and Moulds in Acidic FoodEN ISO 21528-1 Total Enterobacteriaceae Count in SpicesEN ISO 21528-2 Enumeration of Enterobacteriaceae in FoodEN ISO 22117 Quality Assurance for Microbiological Testing LabsEN ISO 22118 Quantification of Listeria monocytogenes in FoodEN ISO 22964 Detection of Cronobacter in Dairy-Based DessertsEN ISO 22964 Detection of Enterobacteriaceae in Powdered MilkEN ISO 29201 Quantification of Virus Particles in Food WastewaterEN ISO 4832 Coliform Count in Processed FoodEN ISO 6222 Enumeration of Aerobic Bacteria in Bottled WaterEN ISO 6579 Detection of Salmonella spp. in SpicesEN ISO 6579-3 Salmonella Serotyping MethodsEN ISO 6887 Preparation of Test Samples and Dilutions for Microbiological TestingEN ISO 6887-1 Preparation of Test Samples from Raw Food MaterialsEN ISO 6887-3 Sample Preparation for Fishery ProductsEN ISO 6887-4 Preparation of Test Samples from Frozen ProductsEN ISO 6887-5 Preparation of Samples from Prepared MealsEN ISO 6887-6 Sample Prep for Dry Foods and PowdersEN ISO 6888-2 Enumeration of Staphylococcus aureus in Egg ProductsEN ISO 6888-3 Detection of Staphylococcus in Ready-to-Eat MealsEN ISO 6888-3 Enumeration of Coagulase-Positive StaphylococciEN ISO 7218 Guidelines for Expression of Results in CFUEN ISO 7218 Guidelines for Plate Count Method in Microbiological LabsEN ISO 7218 Procedures for Colony Counting in MicrobiologyEN ISO 7932 Enumeration of Bacillus cereus in Dehydrated FoodsEN ISO 7937 Enumeration of Clostridium perfringens in GravyEN ISO 8199 General Requirements for Microbiological Methods in WaterEN ISO 8199 Method Validation for Microbiological Water TestingEN ISO 9308-1 Detection of E. coli in Treated WastewaterEN ISO 9308-2 Membrane Filtration for Coliform Bacteria in BeveragesISO 10272-1 Campylobacter Detection in PoultryISO 11290-1 Detection and Enumeration of Listeria monocytogenes in FoodsISO 11290-1 Listeria monocytogenes Detection in Dairy ProductsISO 11290-2 Listeria Count in Cold-Stored FoodsISO 11731 Legionella Detection in Bottled WaterISO 13136 VTEC Detection in Dairy ProductsISO 13720 Pseudomonas spp. Enumeration in Chilled FoodsISO 14189 Enumeration of Sulfite-Reducing Bacteria in WaterISO 15213 Anaerobic Bacteria Testing in Canned FoodISO 15213 Enumeration of Sulfite-Reducing BacteriaISO 15214 Lactic Acid Bacteria Enumeration in Fermented FoodISO 15216-1 Hepatitis A Virus Detection in Frozen BerriesISO 15216-2 Norovirus Detection in Processed VegetablesISO 16211 Anaerobic Sulfite-Reducing Bacteria TestingISO 16212 Fungi Testing in Cosmetic Food IngredientsISO 16266 Pseudomonas aeruginosa in Bottled WaterISO 16649-2 E. coli Enumeration in Raw MeatISO 16649-2 Enumeration of Escherichia coli by Colony-count TechniqueISO 16649-3 Beta-Glucuronidase E. coli DetectionISO 16654 E. coli O157 Detection in Ground BeefISO 17410 Detection of Psychrotrophic Microorganisms in Meat ProductsISO 17410 Psychrotrophic Microorganism Testing in Chilled FoodsISO 17604 Carcass Surface Microbial Sampling in SlaughterhousesISO 17604 Microbiological Swabbing of Animal CarcassesISO 17994 Comparative Testing for Microbiological Water AnalysisISO 18465 Norovirus Testing in ShellfishISO 18593 Surface Swab Microbiological Analysis in Food FacilitiesISO 19020 Food Processing Surface Hygiene MonitoringISO 19036 Uncertainty Estimation in Microbiological CountsISO 21149 Microbiological Examination in Cosmetic Raw Food MaterialsISO 21527-1 Enumeration of Yeasts and Molds in Dried Food ProductsISO 21527-1 Yeast and Mould Count in Low Water Activity FoodsISO 21528-2 Enterobacteriaceae Detection in Food ProductsISO 22964 Cronobacter spp. Testing in Infant FormulaISO 29201 Enteric Virus Quantification in WastewaterISO 4833 Total Viable Count Testing in Food SamplesISO 4833-1 Aerobic Plate Count in Food ProductsISO 6222 Heterotrophic Plate Count in WaterISO 6579 Detection of Salmonella spp. in Food SamplesISO 6579-1 Salmonella spp. Testing in Ready-to-Eat FoodsISO 6887-1 Sample Preparation for Microbiological Examination of FoodISO 6887-2 Microbiological Sample Prep for Dairy ProductsISO 6888-1 Enumeration of Coagulase-Positive Staphylococci (Staphylococcus aureus)ISO 6888-1 Staphylococcus aureus Enumeration in Bakery ProductsISO 6888-2 Staphylococcus aureus in Processed MeatISO 7218 General Requirements for Microbiological ExaminationsISO 7218 Microbiological Counting Methods in Food TestingISO 7218 Standardized Colony Count TechniquesISO 7251 Coliform Bacteria Detection in Drinking WaterISO 7932 Bacillus cereus Enumeration in CerealsISO 7937 Clostridium perfringens Testing in Cooked MeatsISO 7937 Detection and Enumeration of Clostridium perfringens in FoodsISO 8199 Microbiological Analysis of Water QualityISO 9308-1 Coliform and E. coli in Treated Water

EN ISO 21528-2 Enumeration of Enterobacteriaceae in Confectionery: Laboratory Testing Services by Eurolab

Introduction

The food industry is subject to strict regulations regarding the safety and quality of products, including confectionery. One critical aspect of ensuring product safety is the detection of microorganisms, particularly Enterobacteriaceae, which can cause spoilage, contamination, and potentially harm consumers. EN ISO 21528-2 Enumeration of Enterobacteriaceae in Confectionery is a laboratory testing service that helps manufacturers meet regulatory requirements and ensure the quality of their products.

Overview of Relevant Standards

EN ISO 21528-2 is part of the EN ISO 21528 series, which provides methods for the detection and enumeration of Enterobacteriaceae in foodstuffs. This standard is based on ISO 21528-2:2017, which has been adopted by the European Committee for Standardization (CEN) as EN ISO 21528-2.

  • ISO 21528-2:2017 - Methods for detection and enumeration of Enterobacteriaceae in foodstuffs.

  • EN 13813:2006 - Microbiology of food and animal feeding stuffs. Horizontal method for the determination of colony-counting at 30C, using a pour plate technique.

    • Standard Development Organizations

    The development of standards like EN ISO 21528-2 is a collaborative effort between standard development organizations (SDOs), regulatory bodies, and industry experts.

  • International Organization for Standardization (ISO): A non-governmental organization that develops and publishes international standards.

  • European Committee for Standardization (CEN): Responsible for developing European standards based on ISO documents.

  • Deutsche Institut für Normung (DIN): German national standards body responsible for standard development.

    • International and National Standards

    This testing service is governed by a range of international and national standards, including:

  • EU Food Law (Regulation (EC) No. 178/2002): Sets out the general principles for food safety.

  • Food Safety Act 1999: UK legislation requiring food businesses to ensure compliance with EU food law.

  • Codex Alimentarius Commission: A joint FAO/WHO body that develops international food standards.

    • Standard Compliance Requirements

    Manufacturers must comply with relevant regulations and standards, which may vary depending on the country or region. Eurolabs laboratory testing services can help companies meet these requirements.

    Why EN ISO 21528-2 is Needed

    Enterobacteriaceae are a type of bacteria that can cause spoilage and contamination in food products, including confectionery. This standard provides a method for detecting and enumerating these microorganisms, ensuring product safety.

    Business and Technical Reasons

  • Ensuring compliance with regulatory requirements.

  • Preventing contamination and spoilage.

  • Maintaining consumer trust and confidence.

  • Protecting company reputation and brand value.

  • Enhancing competitiveness in the market.

    • Consequences of Not Performing This Test

    Failure to detect and enumerate Enterobacteriaceae can lead to:

  • Product recalls or withdrawals.

  • Loss of customer trust and revenue.

  • Damage to company reputation and brand value.

  • Increased regulatory fines or penalties.

    • Industries and Sectors Requiring This Testing

    This testing service is essential for various industries, including:

  • Confectionery manufacturers

  • Bakers

  • Food processors

  • Dairy producers

    • Risk Factors and Safety Implications

    Enterobacteriaceae can cause a range of health issues in consumers, including gastrointestinal problems, allergic reactions, and other complications.

    Quality Assurance and Quality Control Aspects

    Eurolabs laboratory testing services adhere to strict quality assurance and quality control procedures, ensuring accurate and reliable results.

    Contributions to Product Safety and Reliability

    This testing service contributes significantly to product safety and reliability by:

  • Detecting potential contaminants.

  • Ensuring compliance with regulatory requirements.

  • Preventing spoilage and contamination.

    • Competitive Advantages

    Companies that invest in this testing service can gain a competitive edge through:

  • Enhanced reputation and brand value.

  • Improved customer trust and confidence.

  • Increased market share and revenue.

  • Reduced regulatory risks and penalties.

    • Cost-Benefit Analysis

    While the cost of this testing service may seem high, it is essential to consider the benefits of ensuring product safety, compliance with regulations, and maintaining consumer trust.

    Step-by-Step Explanation of the Test

    The EN ISO 21528-2 test involves several steps:

    1. Sampling: Collecting representative samples from the food product.

    2. Homogenization: Mixing the sample to ensure uniform distribution of microorganisms.

    3. Enrichment: Allowing the microorganisms to grow and multiply in a nutrient-rich environment.

    4. Detection: Using selective media to detect Enterobacteriaceae.

    5. Enumeration: Counting the number of Enterobacteriaceae colonies.

    Equipment and Materials Required

    This testing service requires specialized equipment, including:

  • Microbiological incubators

  • Autoclaves

  • Spectrophotometers

    • And materials, such as:

  • Selective media

  • Growth factors

  • Reagents for enrichment and detection

    • Test Procedure

    The test procedure involves the following steps:

    1. Preparation of selective medium: Preparing the selective medium according to the manufacturers instructions.

    2. Inoculation: Inoculating the sample onto the selective medium.

    3. Incubation: Incubating the inoculated plates at 30C for 24-48 hours.

    4. Counting colonies: Counting the number of Enterobacteriaceae colonies.

    Test Limitations

    This testing service has several limitations, including:

  • Sample size and representation

  • Environmental factors affecting microorganism growth

    • Quality Control Measures

    Eurolabs laboratory testing services adhere to strict quality control procedures to ensure accurate and reliable results.

    Interpretation of Results

    The results of this testing service provide information on the presence and quantity of Enterobacteriaceae in the food product, enabling companies to take corrective action if necessary.

    Reporting and Record-Keeping

    Eurolab provides detailed reports and records for each test, including:

  • Sample identification

  • Test methodology

  • Results and interpretation

  • Recommendations for further action

    • Test Validation

    This testing service is validated through regular proficiency testing and calibration to ensure accuracy and reliability.

    Accreditation and Certification

    Eurolabs laboratory testing services are accredited by national accreditation bodies, such as the United Kingdom Accreditation Service (UKAS).

    Continuous Improvement

    Eurolab continually monitors and improves its testing services to ensure compliance with the latest standards and regulations.

    Conclusion

    EN ISO 21528-2 Enumeration of Enterobacteriaceae in Confectionery is a critical laboratory testing service that helps companies ensure product safety, comply with regulatory requirements, and maintain consumer trust. Eurolabs expert team provides high-quality testing services to help companies achieve these goals.

    This comprehensive guide has provided detailed information on the standard, its requirements, and the importance of this testing service in ensuring product safety and compliance.

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