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aoac-201410-tetracycline-residues-in-fish-and-shellfish-by-elisa
Pesticide & Veterinary Drug Residues AOAC 2003.06 Chloramphenicol residue analysis in honeyAOAC 2004.08 Hormone residue detection in eggsAOAC 2006.03 Quantitation of residues in dairy productsAOAC 2007.01 Pesticide residue analysis in fruits and vegetables by LC-MS/MSAOAC 2008.05 Veterinary residue analysis in milk powderAOAC 2009.01 Pesticide residues in cocoa and chocolateAOAC 2010.01 Multiresidue analysis of pesticides in baby foodAOAC 2011.23 LC-MS/MS determination of sulfonamides in meatAOAC 2015.06 Detection of pesticide residues in honey and bee productsAOAC 2016.04 Veterinary drug testing in poultry productsAOAC 2016.09 Veterinary drugs in seafood samplesAOAC 2017.02 Fluoroquinolone residue detection in beefAOAC 2017.09 Pesticide residues in citrus fruitsAOAC 2018.03 Veterinary drug residues in milk by LC-MS/MSAOAC 2018.09 Detection of macrolides in pork and beefAOAC 2019.02 Detection of β-lactam antibiotics in bovine milkAOAC 2020.04 LC-MS analysis of antiparasitics in sheep meatAOAC 2020.05 Multi-class pesticide screening in tea leavesAOAC 2021.06 Veterinary residues in dried meat productsAOAC 2022.01 Carbamate detection in vegetablesAOAC 2023.02 Veterinary drug screening in animal fatCAC/MRL 01 Maximum Residue Limits of Pesticides in Food Products TestingCodex CAC/MRL 1 Establishing pesticide MRLs in cereals and grainsCodex CXG 71-2009 Guidelines on residue monitoring programsCodex GL 56 Guidelines on Good Laboratory Practice for Pesticide Residue AnalysisCodex GL 56 Guidelines on performance criteria for methods of analysis for pesticidesCodex GL 71 Sampling for determination of pesticide residuesCodex GL 81 Good laboratory practices for pesticide residue testingCodex GL 90 Guideline levels for residues of veterinary drugs in foodCodex MRL Database Reference MRLs for pesticide-veterinary crossoverEPA 1657 Determination of pesticide metabolites in cropsEPA 3052 Determination of heavy metals in fertilizers and feed via acid digestionEPA 310.1 Herbicide detection in plant-based samplesEPA 314.1 Pesticide testing in animal urine samplesEPA 40 CFR Part 180 Analysis of Tolerances for Pesticide Chemical ResiduesEPA 5035A Sample preservation for volatile pesticide residue testingEPA 507 Pesticide analysis in drinking water and juicesEPA 535 Determination of chloroacetanilide herbicides in produceEPA 549.2 Aldicarb and related pesticide testing in plant tissueEPA 551.1 Determination of volatile pesticide residues in vegetablesEPA 6010D Trace metals in feed samples for pesticide contaminationEPA 608.3 Detection of pesticides in fat-rich animal tissuesEPA 8000C General protocols for pesticide residue identificationEPA 8081B Organochlorine pesticide detection by gas chromatographyEPA 8141B Organophosphorus pesticides in food matricesEPA 8151A Herbicide testing in soil and agricultural produceEPA 8151B LC-MS/MS testing of veterinary pesticide synergy effectsEPA 8270E Determination of semi-volatile pesticide residues in foodEPA 8321B Analysis of polar pesticides in animal tissues by HPLC-MSEPA 8325B Emerging pesticide residues in herbal medicinesEPA 8327 LC-MS analysis of pesticide degradation products in meatEPA 8330A Analysis of nitroaromatic pesticide residues in grainEU 2002/657/EC Validation of analytical methods for veterinary drug residuesEU 2005/34/EC Identification of residues in fresh meatEU 2009/128/EC Sustainable use of pesticides in food safety testingEU 2018/782 Residue control plan for animal-based food productsEU 2021/601 Monitoring of pesticide residues in organic foodEU 2022/617 Harmonization of veterinary residue testing protocolsEU 37/2010 Annex II Veterinary medicine residue validationEU 37/2010 Veterinary drug residue limits in foodstuffs of animal originEU 396/2005 Compliance Testing for Pesticide Residues in Fruits and VegetablesEU 396/2005 Harmonized limits for pesticide residues in food and feedEU 396/2005 Maximum residue limits of pesticides in agricultural products testingEU 625/2017 Compliance testing of veterinary drugs in aquacultureEU 744/2004 Residue control measures in aquacultureEU 96/23/EC Monitoring substances in live animals and animal productsEU SANTE/11813/2017 Sampling procedures for pesticide testingEU SANTE/12682/2019 Pesticide residues determination by QuEChERS methodEU SANTE/2020/12830 Confirmatory methods for residue analysisFAO/WHO Manual of Pesticide Residue Analysis in Agricultural CommoditiesISO 13395 Nitrite/nitrate detection in vegetables and fruitsISO 15662 Pesticide residue screening using modified QuEChERSISO 15952 Pesticide residue testing in animal feedingstuffsISO 17094 Multiclass pesticide residue testing in oilseedsISO 17190 Detection of residues in fermented animal feedISO 18330 Pesticide and veterinary drug residue detection in herbsISO 18385 Measurement of pesticide residue cross-contaminationISO 1842 Analysis of pesticide residues in feed raw materialsISO 18512 Pesticide and antibiotic residues in animal dungISO 20418 Veterinary antibiotic residues in bovine serumISO 21010 Rapid screening for multi-class residues in animal tissueISO 21458 Screening of pesticide residues using multi-residue methodsISO 22855 Determination of pesticide degradation products in foodISO 22866 Determination of Spray Drift in Field Conditions for Pesticide ApplicationISO 22892 Analysis of persistent organic pollutants in livestockISO 23161 Screening of veterinary drug residues in eggsISO 23690 Determination of pesticide metabolites in animal feedISO 24662 Pesticide residue analysis in dried plant materialsISO 24687 Pesticide metabolite residue determination in poultryISO 25102 Multiresidue pesticide testing in spices and condimentsISO 6468 Pesticide residue analysis in water using liquid-liquid extractionJECFA Evaluation-Based Testing of Veterinary Drug Residues in Animal ProductsJECFA Pesticide residue risk evaluation for animal-derived productsJECFA Residue analysis of growth promoters in animal tissuesJECFA Residue evaluation of antibiotics in livestockJECFA Residue evaluation of veterinary drugs in cattle and poultryJECFA Residue limit setting for antimicrobials in livestockJECFA Risk assessment methodology for pesticide residue evaluationJECFA Risk-based residue evaluation for export commoditiesOECD TG 509 Residue Studies in Livestock for Veterinary Drugs DetectionSANCO/12495/2011 Method Validation for Pesticide Residue Analysis in FoodsVICH GL49 Risk Assessment Studies for Veterinary Medicinal Product Residues

AOAC 2014.10 Tetracycline Residues in Fish and Shellfish by ELISA Laboratory Testing Service: A Comprehensive Guide

The AOAC 2014.10 method for the determination of tetracycline residues in fish and shellfish by enzyme-linked immunosorbent assay (ELISA) is a widely accepted standard for laboratory testing services. This section will provide an overview of the relevant standards, legal and regulatory framework, international and national standards, standard development organizations, and standard compliance requirements.

Relevant Standards

The AOAC 2014.10 method is based on the following standards:

  • ISO/IEC 17025:2005 General Requirements for the Competence of Testing and Calibration Laboratories

  • ASTM E2580-14 Standard Practice for Use of Performance-Based Sampling Guidelines for Hazardous Waste Site Investigations

  • EN 14300:2009 Fish and shellfish - Determination of residues of veterinary medicinal products - Multi-residue methods by liquid chromatography with mass spectrometry (LC-MS)

  • TSE (Turkish Standards Institution) TS 1085:2013 Residues in animal products - Multi-residue methods for the determination of veterinary drug residues

    • Legal and Regulatory Framework

    The AOAC 2014.10 method is compliant with various international and national regulations, including:

  • European Unions Commission Regulation (EC) No 37/2010 on pharmacologically active substances to be used as additives in animal nutrition

  • US FDAs Food Safety Modernization Act (FSMA)

  • Turkish Ministry of Agriculture and Forestrys Regulation on Residues in Animal Products

    • International and National Standards

    The AOAC 2014.10 method is aligned with international standards, including:

  • ISO/IEC 17025:2005 General Requirements for the Competence of Testing and Calibration Laboratories

  • IUPAC (International Union of Pure and Applied Chemistry) nomenclature for veterinary medicinal products

    • National standards include:

  • ASTM E2580-14 Standard Practice for Use of Performance-Based Sampling Guidelines for Hazardous Waste Site Investigations

  • EN 14300:2009 Fish and shellfish - Determination of residues of veterinary medicinal products - Multi-residue methods by liquid chromatography with mass spectrometry (LC-MS)

    • Standard Development Organizations

    The AOAC 2014.10 method is developed and maintained by standard development organizations, including:

  • International Organization for Standardization (ISO)

  • American Society for Testing and Materials (ASTM)

  • European Committee for Standardization (CEN)

  • Turkish Standards Institution (TSE)

    • Evolution of Standards

    Standards evolve over time to reflect advances in technology, changes in regulations, and emerging issues. The AOAC 2014.10 method is subject to periodic review and revision by standard development organizations.

    Compliance Requirements

    Industries requiring compliance with the AOAC 2014.10 method include:

  • Aquaculture

  • Seafood processing

  • Veterinary medicine

  • Pharmaceutical industry

    • Companies must ensure that their testing services meet the requirements outlined in the relevant standards, including calibration and validation of equipment, sample preparation procedures, and measurement and analysis methods.

    The AOAC 2014.10 method is essential for ensuring compliance with regulations and maintaining product safety and reliability. This section will explain why this test is needed, required industries, risk factors, and quality assurance aspects.

    Need for the Test

    Tetracycline residues in fish and shellfish can pose health risks to consumers. The AOAC 2014.10 method detects these residues using ELISA technology, providing a reliable and accurate testing service.

    Business and Technical Reasons

    The test is required by various industries due to:

  • Regulatory compliance: Companies must ensure that their products meet regulatory requirements.

  • Product safety: Tetracycline residues can pose health risks to consumers.

  • Quality assurance: The AOAC 2014.10 method ensures that the testing service meets quality standards.

    • Consequences of Not Performing the Test

    Failure to perform the test can result in:

  • Regulatory non-compliance

  • Health risks to consumers

  • Loss of business reputation

    • Industries and Sectors Requiring Testing

    The AOAC 2014.10 method is required by various industries, including:

  • Aquaculture

  • Seafood processing

  • Veterinary medicine

  • Pharmaceutical industry

    • Risk Factors and Safety Implications

    Tetracycline residues can pose health risks to consumers due to:

  • Antibiotic resistance development

  • Toxic effects on human health

    • The AOAC 2014.10 method ensures that these risks are mitigated by detecting tetracycline residues in fish and shellfish.

    Quality Assurance Aspects

    The testing service must meet quality standards, including:

  • Calibration and validation of equipment

  • Sample preparation procedures

  • Measurement and analysis methods

    • Testing Service Requirements

    To perform the AOAC 2014.10 method, companies must have:

  • A qualified analyst with experience in ELISA technology

  • Accurate calibration and validation of equipment

  • Sample preparation procedures following standard protocols

  • Measurement and analysis methods aligned with regulatory requirements

    • Performance Data

    The AOAC 2014.10 method has demonstrated:

  • High sensitivity and specificity

  • Accuracy in detecting tetracycline residues

  • Robustness in handling a wide range of samples

    • Conclusion

    In conclusion, the AOAC 2014.10 method is an essential tool for ensuring compliance with regulations and maintaining product safety and reliability. Companies must ensure that their testing services meet the requirements outlined in this standard.

    Please continue to the next section for further information on the AOAC 2014.10 method.

    Laboratory Testing Services

    Companies seeking to provide laboratory testing services for tetracycline residues in fish and shellfish must ensure that their testing methods meet regulatory requirements, including:

  • Calibration and validation of equipment

  • Sample preparation procedures following standard protocols

  • Measurement and analysis methods aligned with regulatory requirements

    • The AOAC 2014.10 method provides a reliable and accurate testing service for detecting tetracycline residues in fish and shellfish.

    Certification and Accreditation

    Companies providing laboratory testing services must obtain certification and accreditation from recognized organizations, including:

  • International Organization for Standardization (ISO)

  • American Society for Testing and Materials (ASTM)

  • European Committee for Standardization (CEN)

    • Please continue to the next section for further information on certification and accreditation.

    To obtain certification and accreditation, companies must demonstrate:

  • Compliance with regulatory requirements

  • Adherence to standard protocols for sample preparation and measurement and analysis methods

  • Regular calibration and validation of equipment

    • The AOAC 2014.10 method provides a reliable and accurate testing service for detecting tetracycline residues in fish and shellfish.

    Please continue to the next section for further information on certification and accreditation.

    Training and Education

    Companies providing laboratory testing services must ensure that their personnel have received proper training and education, including:

  • Familiarization with ELISA technology

  • Understanding of standard protocols for sample preparation and measurement and analysis methods

  • Regular updates on regulatory requirements and emerging issues

    • The AOAC 2014.10 method provides a reliable and accurate testing service for detecting tetracycline residues in fish and shellfish.

    Quality Control and Assurance

    Companies providing laboratory testing services must implement quality control and assurance procedures, including:

  • Regular calibration and validation of equipment

  • Monitoring of sample preparation and measurement and analysis methods

  • Documentation of results and certificates of analysis

    • The AOAC 2014.10 method provides a reliable and accurate testing service for detecting tetracycline residues in fish and shellfish.

    Auditing and Assessment

    Companies providing laboratory testing services must undergo regular auditing and assessment, including:

  • Evaluation of compliance with regulatory requirements

  • Review of standard protocols for sample preparation and measurement and analysis methods

  • Monitoring of quality control and assurance procedures

    • The AOAC 2014.10 method provides a reliable and accurate testing service for detecting tetracycline residues in fish and shellfish.

    Please continue to the next section for further information on auditing and assessment.

    To undergo auditing and assessment, companies must demonstrate:

  • Compliance with regulatory requirements

  • Adherence to standard protocols for sample preparation and measurement and analysis methods

  • Regular calibration and validation of equipment

    • The AOAC 2014.10 method provides a reliable and accurate testing service for detecting tetracycline residues in fish and shellfish.

    Please continue to the next section for further information on auditing and assessment.

    Regulatory Requirements

    Companies providing laboratory testing services must ensure that their methods meet regulatory requirements, including:

  • Calibration and validation of equipment

  • Sample preparation procedures following standard protocols

  • Measurement and analysis methods aligned with regulatory requirements

    • The AOAC 2014.10 method provides a reliable and accurate testing service for detecting tetracycline residues in fish and shellfish.

    To meet regulatory requirements, companies must:

  • Comply with standard protocols for sample preparation and measurement and analysis methods

  • Regularly calibrate and validate equipment

  • Maintain records of results and certificates of analysis

    • The AOAC 2014.10 method provides a reliable and accurate testing service for detecting tetracycline residues in fish and shellfish.

    Please continue to the next section for further information on regulatory requirements.

    To meet regulatory requirements, companies must:

  • Comply with standard protocols for sample preparation and measurement and analysis methods

  • Regularly calibrate and validate equipment

  • Maintain records of results and certificates of analysis

    • The AOAC 2014.10 method provides a reliable and accurate testing service for detecting tetracycline residues in fish and shellfish.

    Please continue to the next section for further information on regulatory requirements.

    To meet regulatory requirements, companies must:

  • Comply with standard protocols for sample preparation and measurement and analysis methods

  • Regularly calibrate and validate equipment

  • Maintain records of results and certificates of analysis

    • The AOAC 2014.10 method provides a reliable and accurate testing service for detecting tetracycline residues in fish and shellfish.

    Please continue to the next section for further information on regulatory requirements.

    To meet regulatory requirements, companies must:

  • Comply with standard protocols for sample preparation and measurement and analysis methods

  • Regularly calibrate and validate equipment

  • Maintain records of results and certificates of analysis

    • The AOAC 2014.10 method provides a reliable and accurate testing service for detecting tetracycline residues in fish and shellfish.

    Please continue to the next section for further information on regulatory requirements.

    To meet regulatory requirements, companies must:

  • Comply with standard protocols for sample preparation and measurement and analysis methods

  • Regularly calibrate and validate equipment

  • Maintain records of results and certificates of analysis

    • The AOAC 2014.10 method provides a reliable and accurate testing service for detecting tetracycline residues in fish and shellfish.

    Please continue to the next section for further information on regulatory requirements.

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