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iso-13395-nitritenitrate-detection-in-vegetables-and-fruits
Pesticide & Veterinary Drug Residues AOAC 2003.06 Chloramphenicol residue analysis in honeyAOAC 2004.08 Hormone residue detection in eggsAOAC 2006.03 Quantitation of residues in dairy productsAOAC 2007.01 Pesticide residue analysis in fruits and vegetables by LC-MS/MSAOAC 2008.05 Veterinary residue analysis in milk powderAOAC 2009.01 Pesticide residues in cocoa and chocolateAOAC 2010.01 Multiresidue analysis of pesticides in baby foodAOAC 2011.23 LC-MS/MS determination of sulfonamides in meatAOAC 2014.10 Tetracycline residues in fish and shellfish by ELISAAOAC 2015.06 Detection of pesticide residues in honey and bee productsAOAC 2016.04 Veterinary drug testing in poultry productsAOAC 2016.09 Veterinary drugs in seafood samplesAOAC 2017.02 Fluoroquinolone residue detection in beefAOAC 2017.09 Pesticide residues in citrus fruitsAOAC 2018.03 Veterinary drug residues in milk by LC-MS/MSAOAC 2018.09 Detection of macrolides in pork and beefAOAC 2019.02 Detection of β-lactam antibiotics in bovine milkAOAC 2020.04 LC-MS analysis of antiparasitics in sheep meatAOAC 2020.05 Multi-class pesticide screening in tea leavesAOAC 2021.06 Veterinary residues in dried meat productsAOAC 2022.01 Carbamate detection in vegetablesAOAC 2023.02 Veterinary drug screening in animal fatCAC/MRL 01 Maximum Residue Limits of Pesticides in Food Products TestingCodex CAC/MRL 1 Establishing pesticide MRLs in cereals and grainsCodex CXG 71-2009 Guidelines on residue monitoring programsCodex GL 56 Guidelines on Good Laboratory Practice for Pesticide Residue AnalysisCodex GL 56 Guidelines on performance criteria for methods of analysis for pesticidesCodex GL 71 Sampling for determination of pesticide residuesCodex GL 81 Good laboratory practices for pesticide residue testingCodex GL 90 Guideline levels for residues of veterinary drugs in foodCodex MRL Database Reference MRLs for pesticide-veterinary crossoverEPA 1657 Determination of pesticide metabolites in cropsEPA 3052 Determination of heavy metals in fertilizers and feed via acid digestionEPA 310.1 Herbicide detection in plant-based samplesEPA 314.1 Pesticide testing in animal urine samplesEPA 40 CFR Part 180 Analysis of Tolerances for Pesticide Chemical ResiduesEPA 5035A Sample preservation for volatile pesticide residue testingEPA 507 Pesticide analysis in drinking water and juicesEPA 535 Determination of chloroacetanilide herbicides in produceEPA 549.2 Aldicarb and related pesticide testing in plant tissueEPA 551.1 Determination of volatile pesticide residues in vegetablesEPA 6010D Trace metals in feed samples for pesticide contaminationEPA 608.3 Detection of pesticides in fat-rich animal tissuesEPA 8000C General protocols for pesticide residue identificationEPA 8081B Organochlorine pesticide detection by gas chromatographyEPA 8141B Organophosphorus pesticides in food matricesEPA 8151A Herbicide testing in soil and agricultural produceEPA 8151B LC-MS/MS testing of veterinary pesticide synergy effectsEPA 8270E Determination of semi-volatile pesticide residues in foodEPA 8321B Analysis of polar pesticides in animal tissues by HPLC-MSEPA 8325B Emerging pesticide residues in herbal medicinesEPA 8327 LC-MS analysis of pesticide degradation products in meatEPA 8330A Analysis of nitroaromatic pesticide residues in grainEU 2002/657/EC Validation of analytical methods for veterinary drug residuesEU 2005/34/EC Identification of residues in fresh meatEU 2009/128/EC Sustainable use of pesticides in food safety testingEU 2018/782 Residue control plan for animal-based food productsEU 2021/601 Monitoring of pesticide residues in organic foodEU 2022/617 Harmonization of veterinary residue testing protocolsEU 37/2010 Annex II Veterinary medicine residue validationEU 37/2010 Veterinary drug residue limits in foodstuffs of animal originEU 396/2005 Compliance Testing for Pesticide Residues in Fruits and VegetablesEU 396/2005 Harmonized limits for pesticide residues in food and feedEU 396/2005 Maximum residue limits of pesticides in agricultural products testingEU 625/2017 Compliance testing of veterinary drugs in aquacultureEU 744/2004 Residue control measures in aquacultureEU 96/23/EC Monitoring substances in live animals and animal productsEU SANTE/11813/2017 Sampling procedures for pesticide testingEU SANTE/12682/2019 Pesticide residues determination by QuEChERS methodEU SANTE/2020/12830 Confirmatory methods for residue analysisFAO/WHO Manual of Pesticide Residue Analysis in Agricultural CommoditiesISO 15662 Pesticide residue screening using modified QuEChERSISO 15952 Pesticide residue testing in animal feedingstuffsISO 17094 Multiclass pesticide residue testing in oilseedsISO 17190 Detection of residues in fermented animal feedISO 18330 Pesticide and veterinary drug residue detection in herbsISO 18385 Measurement of pesticide residue cross-contaminationISO 1842 Analysis of pesticide residues in feed raw materialsISO 18512 Pesticide and antibiotic residues in animal dungISO 20418 Veterinary antibiotic residues in bovine serumISO 21010 Rapid screening for multi-class residues in animal tissueISO 21458 Screening of pesticide residues using multi-residue methodsISO 22855 Determination of pesticide degradation products in foodISO 22866 Determination of Spray Drift in Field Conditions for Pesticide ApplicationISO 22892 Analysis of persistent organic pollutants in livestockISO 23161 Screening of veterinary drug residues in eggsISO 23690 Determination of pesticide metabolites in animal feedISO 24662 Pesticide residue analysis in dried plant materialsISO 24687 Pesticide metabolite residue determination in poultryISO 25102 Multiresidue pesticide testing in spices and condimentsISO 6468 Pesticide residue analysis in water using liquid-liquid extractionJECFA Evaluation-Based Testing of Veterinary Drug Residues in Animal ProductsJECFA Pesticide residue risk evaluation for animal-derived productsJECFA Residue analysis of growth promoters in animal tissuesJECFA Residue evaluation of antibiotics in livestockJECFA Residue evaluation of veterinary drugs in cattle and poultryJECFA Residue limit setting for antimicrobials in livestockJECFA Risk assessment methodology for pesticide residue evaluationJECFA Risk-based residue evaluation for export commoditiesOECD TG 509 Residue Studies in Livestock for Veterinary Drugs DetectionSANCO/12495/2011 Method Validation for Pesticide Residue Analysis in FoodsVICH GL49 Risk Assessment Studies for Veterinary Medicinal Product Residues

ISO 13395 Nitrite/Nitrate Detection in Vegetables and Fruits Laboratory Testing Service: A Comprehensive Guide

The detection of nitrites and nitrates in vegetables and fruits is a critical aspect of food safety and quality control. The International Organization for Standardization (ISO) has developed the standard ISO 13395, which provides guidelines for the detection of nitrites and nitrates in these products.

Legal and Regulatory Framework

The legal and regulatory framework surrounding this testing service is governed by various national and international standards. In Europe, the European Commissions Regulation (EC) No 396/2005 sets maximum levels for residues of pesticide active substances in or on food and feed. The regulation requires that all food and feed products comply with these limits.

International and National Standards

The following international and national standards apply to this specific laboratory test:

  • ISO 13395:2011 (Nitrites and nitrates - Determination of nitrite and nitrate in vegetables, fruits, and their products)

  • ASTM D6723-01 (Standard Test Method for Nitrate and Nitrite in Fruits and Vegetables)

  • EN 14172 (Determination of nitrate and nitrite in water intended for human consumption)

  • TSE 1149 (Nitrites and nitrates - Determination of nitrite and nitrate in vegetables, fruits, and their products)

    • Standard Development Organizations

    The standard development organizations involved in the creation of ISO 13395 include:

  • International Organization for Standardization (ISO)

  • American Society for Testing and Materials (ASTM)

  • European Committee for Standardization (CEN)

  • Turkish Standards Institution (TSE)

    • Evolution of Standards

    Standards evolve over time to reflect new scientific knowledge, technological advancements, and changes in regulatory requirements. The ISO 13395 standard has undergone several revisions since its initial publication in 1996.

    Standard Numbers and Scope

    The following are the relevant standard numbers and their scope:

  • ISO 13395:2011 (Nitrites and nitrates - Determination of nitrite and nitrate in vegetables, fruits, and their products)

    • Scope: This International Standard specifies a method for the determination of nitrite and nitrate in vegetables, fruits, and their products.

  • ASTM D6723-01 (Standard Test Method for Nitrate and Nitrite in Fruits and Vegetables)

    • Scope: This test method is used to determine the amount of nitrate and nitrite in fruits and vegetables.

    Industry-Specific Examples

    The following industries require this testing service:

  • Fresh produce industry

  • Processed food industry

  • Beverage industry

    • Quality Assurance and Quality Control

    This testing service requires a quality assurance system that ensures accurate and reliable results. The laboratory must have a quality control program in place to monitor the performance of the test method.

    Consequences of Not Performing This Test

    Failure to perform this test can result in the following consequences:

  • Consumer exposure to toxic levels of nitrites and nitrates

  • Contamination of food products with high levels of nitrites and nitrates

  • Economic losses due to recalls and product destruction

    • Risk Factors and Safety Implications

    The following are the risk factors and safety implications associated with this testing service:

  • Exposure to toxic chemicals during sample preparation and analysis

  • Risk of inaccurate results due to contamination or equipment malfunction

  • Consequences of non-compliance with regulatory requirements

    • The ISO 13395 standard specifies a method for the determination of nitrite and nitrate in vegetables, fruits, and their products. The following are the test conditions and methodology:

    1. Sample Preparation

    Weigh approximately 10 g of sample into a 50 ml tube.

    Add 20 ml of distilled water to the tube.

    Shake the mixture for 5 minutes.

    2. Testing Parameters and Conditions

    Parameter Condition

    --- ---

    Temperature 25C 1C

    Humidity 60 10 RH

    Pressure Atmospheric pressure

    3. Measurement and Analysis Methods

    Use a spectrophotometer to measure the absorbance of the sample at 220 nm.

    Calculate the concentration of nitrite and nitrate using the Beer-Lambert law.

    The following are the requirements for test reporting and documentation:

    1. Report Format

    The report must include the name, address, and contact information of the laboratory.

    The report must include the name and address of the customer.

    The report must include a description of the sample.

    The report must include the results of the test.

    2. Interpretation of Test Results

    Interpret the results in accordance with the relevant regulatory requirements.

    Industry-Specific Examples

    The following industries require this testing service:

  • Fresh produce industry

  • Processed food industry

  • Beverage industry

    • Persuasive Summary

    The detection of nitrites and nitrates in vegetables and fruits is a critical aspect of food safety and quality control. The ISO 13395 standard provides guidelines for the detection of these compounds. Laboratories must have a quality assurance system in place to ensure accurate and reliable results.

    By following this guide, laboratories can ensure that they are meeting the requirements of the ISO 13395 standard and providing high-quality testing services to their customers.

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