Comprehensive Guide to CEN EN 15789 Ergot Alkaloid Detection Methods Laboratory Testing Service
Standard-Related Information
CEN EN 15789 Ergot Alkaloid Detection Methods testing is governed by a set of international and national standards that ensure the accuracy, precision, and reliability of laboratory results. The primary standard governing this testing service is CEN EN 15789:2015, which outlines the requirements for detecting ergot alkaloids in foodstuffs.
International and National Standards
Standard Development Organizations
The development of CEN EN 15789 Ergot Alkaloid Detection Methods testing standards is the responsibility of various standard development organizations, including:
Evolution and Updates
Standards evolve over time to reflect advances in technology, changes in regulatory requirements, or emerging safety concerns. CEN EN 15789 Ergot Alkaloid Detection Methods testing standards are reviewed and updated periodically by the relevant standard development organizations.
Scope and Compliance Requirements
The scope of CEN EN 15789 Ergot Alkaloid Detection Methods testing includes:
Compliance with these standards is mandatory for all laboratories performing CEN EN 15789 Ergot Alkaloid Detection Methods testing. Industry sectors requiring this testing include:
Standard Requirements and Needs
The primary reason for conducting CEN EN 15789 Ergot Alkaloid Detection Methods testing is to ensure the safety of food products by detecting potential ergot alkaloids contamination. This testing serves several purposes, including:
1. Product Safety: Ensures that food products are free from ergot alkaloids, which can be toxic to humans and animals.
2. Regulatory Compliance: Meets regulatory requirements for detecting ergot alkaloids in foodstuffs.
3. Quality Assurance: Verifies the quality of raw materials and final products.
4. Competitive Advantage: Demonstrates commitment to product safety and quality.
Consequences of Not Performing This Test
Failure to conduct CEN EN 15789 Ergot Alkaloid Detection Methods testing can result in:
Test Conditions and Methodology
The test is conducted using a combination of high-performance liquid chromatography (HPLC) with fluorescence detection (F-457nm excitation, E-485nm emission) and mass spectrometry (MS). The testing equipment includes:
Sample Preparation
The sample preparation procedure involves:
1. Grinding: Grinding the sample to a fine powder.
2. Extraction: Extracting the ergot alkaloids using a solvent.
3. Clean-up: Cleaning up the extract using a solid-phase extraction (SPE) cartridge.
Measurement and Analysis Methods
The measurement and analysis methods include:
1. HPLC-FLD: Measuring the peak area of ergot alkaloids at 457nm excitation and 485nm emission.
2. MS: Identifying and quantifying the mass-to-charge ratio (m/z) of ergot alkaloids.
Calibration and Validation Procedures
The calibration and validation procedures involve:
1. Instrument calibration: Calibrating the HPLC system, FLD detector, and MS analyzer.
2. Method validation: Validating the test method using certified reference materials (CRMs).
Quality Control Measures During Testing
Quality control measures during testing include:
1. Blank controls: Running blank samples to monitor interferences.
2. QC samples: Running quality control (QC) samples to verify the accuracy and precision of the test.
Data Collection and Recording Procedures
The data collection and recording procedures involve:
1. Instrument logging: Logging instrument settings, temperature, and other parameters.
2. Sample documentation: Documenting sample information, including identification and storage conditions.
Test Reporting and Documentation
The test report includes:
1. Summary of results: A summary of the test results, including any deviations or anomalies.
2. Raw data: Raw data from the HPLC-FLD and MS analysis.
3. Certification: Certification that the sample meets regulatory requirements.
Persuasive Conclusion
In conclusion, CEN EN 15789 Ergot Alkaloid Detection Methods testing is a critical component of ensuring product safety and quality in food manufacturing, brewing, distilling, and animal feed production. By conducting this testing, laboratories can demonstrate their commitment to regulatory compliance, product safety, and quality assurance.
By following the standard requirements and test conditions outlined in CEN EN 15789 Ergot Alkaloid Detection Methods, laboratories can ensure that their results are accurate, precise, and reliable.
Recommendation
We highly recommend that all laboratories performing CEN EN 15789 Ergot Alkaloid Detection Methods testing adhere to these standards to ensure the accuracy, precision, and reliability of laboratory results.
