EUROLAB
aoac-200111-pyridoxine-vitamin-b6-quantification-in-supplements
Nutritional Labeling & Composition AOAC 2000.12 vitamin K1 determination in baby foodAOAC 2001.03 flavonoid content testing in plant-based nutrition barsAOAC 2001.07 taurine analysis in energy and recovery drinksAOAC 2001.12 vitamin B12 analysis in nutritional yeastAOAC 2001.13 cholesterol quantification in processed meatsAOAC 2002.02 biotin determination in fortified food itemsAOAC 2002.07 steviol glycosides testing in stevia-sweetened itemsAOAC 2002.09 vitamin E (α-tocopherol) analysis in edible oilsAOAC 2003.09 casein to whey ratio testing in milk protein powdersAOAC 2004.05 L-carnitine content testing in sports supplementsAOAC 2005.06 vitamin K determination in infant nutrition productsAOAC 2005.08 lycopene quantification in tomato-based productsAOAC 2007.01 lutein concentration analysis in eye-health supplementsAOAC 2009.01 amino acid profiling for protein supplement labelingAOAC 2009.03 inositol content testing in fortified cerealsAOAC 2010.05 trans fat analysis for label complianceAOAC 2011.01 polyunsaturated fatty acid testing in cooking oilsAOAC 2011.02 protein digestibility analysis for sports nutritionAOAC 2011.14 vitamin D determination in dairy and plant-based drinksAOAC 2011.18 phytonutrient testing in whole-food supplementsAOAC 2011.19 vitamin B complex profile in multivitamin drinksAOAC 2012.11 flavanol testing in cocoa-based nutrition productsAOAC 2012.13 beta-carotene determination in carrot-based baby foodsAOAC 2013.05 choline determination in soy-based nutrition foodsAOAC 2015.06 resistant starch analysis for gut health food claimsAOAC 2016.03 myo-inositol analysis in children’s formula productsAOAC 2017.04 betaine content testing in sports nutrition blendsAOAC 2018.07 omega-6 fatty acid testing in plant-based oilsAOAC 941.15 total sugar testing for natural juice productsAOAC 942.05 lactose quantification in lactose-reduced dairyAOAC 944.12 ethyl alcohol content in ready-to-consume beveragesAOAC 948.13 total solids testing in frozen food productsAOAC 950.46 nitrogen-free extract in pet nutrition labelingAOAC 960.52 calcium determination in dairy-based nutritional drinksAOAC 969.33 protein efficiency ratio testing in infant formulaAOAC 970.65 iron quantification for fortified cereal productsAOAC 974.18 niacinamide testing in vitamin-enriched foodAOAC 974.29 total sugars analysis in sweetened beveragesAOAC 975.03 ethanol content testing in nutritional beveragesAOAC 979.23 antioxidant testing for labeled antioxidant-rich foodsAOAC 979.27 tocopherol (Vitamin E) testing in fortified oilsAOAC 980.13 total solids and moisture content in canned foodsAOAC 983.19 sorbitol content in sugar-free labeled foodsAOAC 984.13 copper concentration analysis for label complianceAOAC 984.13 potassium determination in health drinksAOAC 984.27 chromium quantification in dietary supplement testingAOAC 984.27 niacin testing in multivitamin nutrition tabletsAOAC 984.27 selenium testing in trace mineral supplementsAOAC 985.26 zinc content testing in child nutrition productsAOAC 985.29 based fiber analysis for nutritional labelingAOAC 985.35 vitamin A testing for infant formula labelingAOAC 990.11 pantothenic acid testing in enriched bakery itemsAOAC 991.25 magnesium level determination in dietary supplementsAOAC 991.29 fatty acid profile determination in omega-3 productsAOAC 991.36 caffeine quantification in energy drinksAOAC 991.43 total dietary fiber determination in food productsAOAC 992.06 sodium content analysis for reduced salt label validationAOAC 992.15 vitamin C analysis in fruit juice and beveragesAOAC 992.24 calcium content validation in dairy alternativesAOAC 993.13 lactose analysis in lactose-free labeled productsAOAC 993.14 omega-3 (EPA & DHA) testing in fish oil capsulesAOAC 996.04 iodine testing in iodized salt for label accuracyAOAC 996.06 riboflavin (Vitamin B2) analysis in vitamin drinksAOAC 999.10 thiamine (Vitamin B1) quantification in nutrition productsAOAC 999.13 folic acid determination in fortified breakfast cerealsAOAC 999.14 phosphorus content testing for nutrition claimsCodex Alimentarius compliant carbohydrate profile testing for packaged foodsCodex CXG 2-1985 compliance testing for nutritional labeling accuracyISO 11085 total fat content analysis in cereal productsISO 13496 ash content analysis in dry food commoditiesISO 1442 moisture content determination in nutritional meat productsISO 15705 determination of total organic carbon in food matricesISO 1672 carbohydrate testing by enzymatic method in nutrition studiesISO 17678 compliant protein content determination in milk and dairy productsISO 18330 polyphenol content determination in functional beveragesISO 1841 sodium chloride testing in canned vegetables for labelingISO 1871 total nitrogen determination in high-protein foodsISO 21422 water activity measurement in dried food productsISO 21527-1 microbial stability testing for shelf-life claimsISO 21527-2 yeast and mold count validation in nutrient-dense foodsISO 2171 starch content testing for grain-based nutritional labelsISO 2173 total sugar testing in fruit and vegetable juicesISO 22935 sensory analysis for label claim verification in dairy productsISO 2446 based fat content analysis in liquid milkISO 3071 pH value testing for acidified food labelingISO 3593 dietary fiber content in soy-based nutrition foodsISO 4125 energy value estimation in prepared meal testingISO 6492 based crude protein testing in animal feed supplementsISO 6496 moisture content determination in food labeling complianceISO 6865 crude fiber determination in nutrition-labeled pet foodsISO 763 sodium content testing in meat productsISO 8262-1 gravimetric method for milk fat content testingRegulation (EU) No 1169/2011 nutritional declaration validation testing

Comprehensive Guide to AOAC 2001.11 Pyridoxine (Vitamin B6) Quantification in Supplements Testing Services by Eurolab

Standard-Related Information

AOAC 2001.11 is a widely recognized standard for the quantification of pyridoxine (Vitamin B6) in dietary supplements. This standard is published by the Association of Official Analytical Chemists (AOAC), an international organization that sets standards for analytical chemistry.

The AOAC 2001.11 standard provides a detailed method for the determination of pyridoxine (Vitamin B6) in dietary supplements using high-performance liquid chromatography (HPLC). The standard outlines the sampling procedure, sample preparation, and HPLC conditions required for accurate and precise measurement of pyridoxine (Vitamin B6).

In addition to AOAC 2001.11, other relevant standards that govern this testing service include:

  • ISO 17025:2017 - General requirements for the competence of testing and calibration laboratories

  • EN 14300:2012 - Dietary supplements - Methods of analysis for vitamin B6 (pyridoxine)

  • TSE 1420:2020 - Foodstuffs - Methods of analysis for vitamin B6 (pyridoxine)

    • The legal and regulatory framework surrounding this testing service is governed by national and international laws, regulations, and guidelines. For example:

  • The United States Federal Trade Commission (FTC) requires that dietary supplements contain the amount of pyridoxine (Vitamin B6) stated on the label.

  • In Europe, the European Food Safety Authority (EFSA) sets maximum permissible levels for pyridoxine (Vitamin B6) in foodstuffs.

    • Standard development organizations play a crucial role in setting standards for analytical chemistry. These organizations include:

  • AOAC International

  • International Organization for Standardization (ISO)

  • European Committee for Standardization (CEN)

    • Standards evolve and get updated regularly to reflect new scientific knowledge, advances in technology, and changes in regulatory requirements.

    Standard Requirements and Needs

    AOAC 2001.11 pyridoxine (Vitamin B6) quantification in supplements testing is required by various industries, including:

  • Dietary supplement manufacturers

  • Food processing companies

  • Pharmaceutical firms

  • Regulatory agencies

    • The consequences of not performing this test include:

  • Incorrect labeling or under-labeling of dietary supplements

  • Potential health risks for consumers due to inadequate pyridoxine (Vitamin B6) levels

  • Loss of business reputation and market share due to non-compliance with regulations

    • This testing is essential for ensuring product safety, quality, and compliance with regulatory requirements.

    Test Conditions and Methodology

    The AOAC 2001.11 method involves the following steps:

    1. Sampling: Select a representative sample from the batch or lot.

    2. Sample preparation: Weigh the sample into a suitable container, add reagents, and mix thoroughly.

    3. HPLC analysis: Inject the sample onto an HPLC column, using a suitable mobile phase and detection system.

    The testing equipment and instruments used for this test include:

  • HPLC instrument

  • Column

  • Detector

  • Mobile phase pump

    • The testing environment requirements are as follows:

  • Temperature: 20-25C

  • Humidity: 40-60

  • Pressure: atmospheric pressure

    • Test Reporting and Documentation

    Test results are documented and reported according to the following format:

  • Sample ID

  • Test method (AOAC 2001.11)

  • Analyte (pyridoxine, Vitamin B6)

  • Concentration (mg/g or mg/mL)

  • Precision and accuracy data

  • Certification and accreditation information

    • The report includes an interpretation of test results, certification, and accreditation details.

    Why This Test Should Be Performed

    Performing AOAC 2001.11 pyridoxine (Vitamin B6) quantification in supplements testing provides numerous benefits, including:

  • Ensures product safety and compliance with regulations

  • Provides accurate labeling and quality control

  • Enhances customer confidence and trust

  • Supports international market access and trade facilitation

  • Contributes to innovation and research development

    • Why Eurolab Should Provide This Service

    Eurolab is an expert provider of AOAC 2001.11 pyridoxine (Vitamin B6) quantification in supplements testing services, offering:

  • State-of-the-art equipment and facilities

  • Qualified and certified personnel

  • Accreditation and certification details

  • International recognition and partnerships

  • Quality management systems and procedures

  • Customer service and support capabilities

    • Eurolabs comprehensive guide to AOAC 2001.11 pyridoxine (Vitamin B6) quantification in supplements testing services is designed to provide accurate and reliable results, ensuring product safety and compliance with regulations.

    Conclusion

    AOAC 2001.11 pyridoxine (Vitamin B6) quantification in supplements testing is a critical service that ensures the quality and safety of dietary supplements. Eurolabs comprehensive guide provides a detailed understanding of this standard and its requirements, making it an essential resource for laboratories, manufacturers, and regulatory agencies.

    By following this guide, laboratories can ensure accurate and reliable results, supporting product safety, compliance with regulations, and customer confidence.

    References

    1. AOAC International (2001). Pyridoxine in Dietary Supplements: Official Method 2001.11.

    2. ISO 17025:2017 - General requirements for the competence of testing and calibration laboratories.

    3. EN 14300:2012 - Dietary supplements - Methods of analysis for vitamin B6 (pyridoxine).

    4. TSE 1420:2020 - Foodstuffs - Methods of analysis for vitamin B6 (pyridoxine).

    Appendix

    Table 1: Sampling Procedure

    Sample ID Batch/Lot Number

    --- ---

    001 ABC123

    002 DEF456

    Table 2: HPLC Conditions

    Column Mobile Phase Pump Detector

    --- --- ---

    C18 Water:Acetonitrile (80:20) UV (254 nm)

    Table 3: Test Results

    Sample ID Concentration (mg/g) Precision () Accuracy ()

    --- --- --- ---

    001 10.5 0.2 0.1

    002 12.8 0.3 0.2

    Certification

    Eurolab is certified to ISO 17025:2017, demonstrating its competence in testing and calibration laboratory activities.

    Accreditation

    Eurolab is accredited by the National Accreditation Body (NAB) for AOAC 2001.11 pyridoxine (Vitamin B6) quantification in supplements testing services.

    Contact Us

    For more information on Eurolabs AOAC 2001.11 pyridoxine (Vitamin B6) quantification in supplements testing services, please contact:

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