AOAC 974.18 niacinamide testing in vitamin-enriched food

Comprehensive Guide to AOAC 974.18 Niacinamide Testing in Vitamin-Enriched Food Laboratory Testing Service Provided by Eurolab

AOAC 974.18 is a widely recognized standard for the determination of niacinamide in vitamin-enriched foods. The standard is published by the Association of Official Analytical Chemists (AOAC) and provides a detailed methodology for the accurate measurement of niacinamide content in various food products.

The AOAC 974.18 standard is based on the principles of high-performance liquid chromatography (HPLC) with fluorescence detection. This method offers excellent sensitivity, selectivity, and accuracy for the determination of niacinamide in complex food matrices.

The standard is applicable to a wide range of vitamin-enriched foods, including:

In addition to AOAC 974.18, several other international and national standards govern the testing of niacinamide in vitamin-enriched foods. These include:

The standard development organizations responsible for developing these standards include:

These standards undergo periodic review and revision to ensure they remain relevant and effective in ensuring the accuracy and reliability of niacinamide testing.

AOAC 974.18 niacinamide testing is essential for several industries, including:

The consequences of not performing this test can be severe, including:

The business and technical reasons for conducting AOAC 974.18 niacinamide testing include:

Quality assurance and quality control aspects of AOAC 974.18 niacinamide testing include:

This test contributes significantly to product safety and reliability by ensuring that niacinamide levels are accurately measured and within acceptable limits.

AOAC 974.18 niacinamide testing is conducted using a high-performance liquid chromatography system with fluorescence detection. The following steps outline the detailed methodology:

1. Sample preparation: A representative sample of the vitamin-enriched food product is collected and homogenized.

2. Extraction: The sample is extracted using a solvent suitable for the matrix, such as water or an organic solvent.

3. Chromatography: The extracted sample is injected into the HPLC system, where it passes through a column packed with a stationary phase that separates the niacinamide from other components in the sample.

4. Detection: The separated compounds are detected using fluorescence detection, which provides excellent sensitivity and selectivity for niacinamide.

The testing equipment and instruments used include:

Testing environment requirements include:

Sample preparation procedures involve:

Testing parameters and conditions include:

Measurement and analysis methods involve:

Calibration and validation procedures include:

Quality control measures during testing involve:

Conclusion

AOAC 974.18 niacinamide testing is a widely recognized standard for determining niacinamide levels in vitamin-enriched foods. The standard provides a detailed methodology for accurate measurement using high-performance liquid chromatography with fluorescence detection. This test is essential for ensuring product safety, maintaining compliance with regulatory requirements, and building customer confidence and trust.

The AOAC 974.18 standard is applicable to various industries, including food manufacturers, nutritional supplement manufacturers, infant formula manufacturers, and beverage manufacturers. The consequences of not performing this test can be severe, including accidental overdosing or underdosing of niacinamide in food products, non-compliance with regulatory requirements, and damage to brand reputation.

By following the standard methodology outlined above, laboratories can ensure accurate and reliable measurement of niacinamide levels in vitamin-enriched foods.