EUROLAB
aoac-200301-testing-for-deoxynivalenol-in-corn-based-products
Mycotoxin Testing AOAC 2000.02 Patulin Testing in Apple PureeAOAC 2000.03 Determination of Patulin in Apple JuiceAOAC 2000.05 Patulin Detection in Fermented BeveragesAOAC 2003.02 Measurement of Deoxynivalenol in Wheat FlourAOAC 2003.03 Quantitative Analysis of Mycotoxins in Animal FeedAOAC 2005.06 Determination of Fumonisins by Immunoaffinity and LCAOAC 2005.07 Quantification of Aflatoxins in Tree NutsAOAC 2005.08 Determination of Zearalenone in Animal FeedAOAC 2005.09 Determination of Zearalenone in MaizeAOAC 2007.01 Quantitative Determination of Zearalenone in CornAOAC 2008.01 Fumonisin Testing in Corn GritsAOAC 2008.02 Detection of Fumonisins in Corn Using HPLC-FLDAOAC 2008.02 Quantification of Fumonisin B1 and B2 in MaizeAOAC 2008.03 Multi-Residue Detection of Mycotoxins in Animal FeedAOAC 986.16 Testing of Total Aflatoxins in Corn Using FluorometryAOAC 991.31 Aflatoxin Screening in Feed IngredientsAOAC 991.31 Determination of Aflatoxin B1 in Peanut ButterAOAC 991.31 Testing for Total Aflatoxins in Peanuts and CornAOAC 994.08 Screening of Aflatoxins by TLCAOAC 994.09 Detection of Aflatoxins in AlmondsAOAC 998.03 Screening for Aflatoxins in Maize Using Test KitsAOAC 999.07 Detection of Aflatoxin B1 in RiceCEN/TR 16059 General Protocol for Mycotoxin Testing in Food ChainsEN 14123 Determination of Ochratoxin A in Wine by Immunoaffinity ColumnEN 15289 Determination of Ochratoxin A in Cocoa by LCEN 15662 Multimycotoxin Detection in Fruits and Vegetables Using LC-MSEN 15788 Determination of Deoxynivalenol in Baby FoodEN 15792 Detection of Mycotoxins in Tomato PureeEN 15891 Determination of Trichothecenes in Food Using LC-MSEN 15891 HT-2/T-2 Detection in Baby FoodsEN 15891 Measurement of HT-2 and T-2 Toxins in CerealsEN 15891 T-2 and HT-2 Toxin Detection in OatsEN 16571 Testing of Aflatoxin M1 in Milk Products by ELISAEN 16619 Detection of Mycotoxins in Edible OilsEN 17193 Determination of Aflatoxicol in Milk and DairyEN 17194 Determination of Citrinin in Red Yeast Rice Food SupplementsEN 17200 Determination of Mycotoxins in SpicesEN 17201 Determination of Alternaria Toxins in Tomato ProductsEN 17250 Determination of Ergot Alkaloids in Feed by LC-MSEN 17299 Testing of Modified Mycotoxins in Animal FeedEN 17461 Determination of Fusarenon-X in Cereals by LC-MSEN 17462 Rapid Testing of Citrinin in Red Fermented ProductsEN 17463 Screening of Ergot Alkaloids in GrainEN 17521 Mycotoxin Profiling in Complex Matrices Using LC-MS/MSEU 2006/401/EC Mycotoxin Control in Import CommoditiesEU Regulation 1881/2006 Compliance Testing for Mycotoxin Levels in FoodFAO Recommended Methods for Mycotoxin Sampling and DetectionFDA BAM Chapter 19 Mycotoxins Analysis ProtocolsFDA BAM Mycotoxin Analytical Methods for Regulatory MonitoringFDA CFR 21 Part 556 Testing for Mycotoxin Tolerances in Animal FeedISO 14501 Determination of Patulin in Fruit Juices by HPLCISO 15141 Determination of Deoxynivalenol in Cereals by GC-MSISO 16050 Determination of Aflatoxin B1, B2, G1, G2 in CerealsISO 16050 Determination of Aflatoxins in Foodstuffs Using HPLCISO 16050 Testing for Aflatoxins in Herbs and SpicesISO 17375 Determination of Ochratoxin A in Roasted Coffee by HPLCISO 18787 Guidelines for Mycotoxin Sampling in GrainsISO 18856 Determination of Ochratoxin A in Foodstuffs by LC-MS/MSISO 21527 Mold Enumeration for Mycotoxin-Prone FoodsISO 21527-1 Enumeration of Yeasts and Molds for Spoilage RiskISO 24276 Guidelines for Validation of Mycotoxin Detection MethodsISO 24276 Method Validation for Zearalenone DetectionJECFA Limits-Based Mycotoxin Testing in Dried FruitsJECFA Maximum Levels of Mycotoxins in Cereal GrainsJECFA Toxicity Testing for Aflatoxin M1 in Milk

AOAC 2003.01 Testing for Deoxynivalenol in Corn-Based Products: Eurolabs Laboratory Testing Service

AOAC 2003.01 is a widely recognized and accepted standard for testing deoxynivalenol (DON) in corn-based products. DON, also known as vomitoxin, is a mycotoxin produced by Fusarium fungi that can contaminate corn and other grains. The presence of DON in food and feed products poses significant health risks to humans and animals.

International Standards

The international standards governing AOAC 2003.01 testing are:

  • ISO/IEC 17025:2017 General requirements for the competence of testing and calibration laboratories

  • ASTM E2584-09 Standard Practice for Sampling and Sample Handling of Grains and Grain Products for Mycotoxin Analysis

  • EN 14183:2002 Mycotoxins in foodstuffs. Deoxynivalenol (DON) - Method using liquid chromatography with mass spectrometry detection (LC-MS/MS)

    • National Standards

    The national standards governing AOAC 2003.01 testing vary by country:

  • In the United States, the FDA regulates DON levels in corn-based products under 21 CFR Part 109

  • In the European Union, the EU regulates DON levels in corn-based products under Regulation (EC) No 1881/2006

  • In Australia and New Zealand, the regulatory bodies are Food Standards Australia New Zealand (FSANZ) and the Australian Government Department of Agriculture

    • Standard Development Organizations

    The standard development organizations responsible for AOAC 2003.01 testing are:

  • The Association of Official Analytical Chemists (AOAC)

  • The International Organization for Standardization (ISO)

  • The American Society for Testing and Materials (ASTM)

    • Evolution of Standards

    Standards evolve and get updated to reflect advances in technology, changes in regulatory requirements, and new research findings. For example, the latest version of AOAC 2003.01 was published in 2015 to reflect improvements in analytical methods and increased sensitivity.

    Standard Numbers and Scope

    The relevant standard numbers and their scope are:

  • AOAC 2003.01: Deoxynivalenol (DON) - Method using liquid chromatography with mass spectrometry detection (LC-MS/MS)

  • ISO 21591:2014 Mycotoxins in foodstuffs - Determination of deoxynivalenol (DON) and zearalenone (ZEN) by liquid chromatography with tandem mass spectrometry (LC-MS/MS)

    • Standard Compliance Requirements

    Compliance with AOAC 2003.01 testing is required for various industries, including:

  • Food manufacturers

  • Grain traders

  • Feed producers

  • Regulatory agencies

    • Consequences of non-compliance can include product recalls, financial penalties, and damage to reputation.

    Standard-Related Information Summary

    Standard Description

    --- ---

    AOAC 2003.01 Deoxynivalenol (DON) - Method using liquid chromatography with mass spectrometry detection (LC-MS/MS)

    ISO 21591:2014 Mycotoxins in foodstuffs - Determination of deoxynivalenol (DON) and zearalenone (ZEN) by liquid chromatography with tandem mass spectrometry (LC-MS/MS)

    ASTM E2584-09 Standard Practice for Sampling and Sample Handling of Grains and Grain Products for Mycotoxin Analysis

    Why is AOAC 2003.01 Testing Required?

    AOAC 2003.01 testing is required to ensure the safety and quality of corn-based products. DON contamination can cause health problems in humans, including nausea, vomiting, and diarrhea.

    Business and Technical Reasons for Conducting AOAC 2003.01 Testing

    Conducting AOAC 2003.01 testing provides several benefits:

  • Ensures compliance with regulatory requirements

  • Protects consumer health and safety

  • Maintains product quality and reputation

  • Reduces the risk of product recalls and financial penalties

    • Consequences of Not Performing AOAC 2003.01 Testing

    Failure to conduct AOAC 2003.01 testing can result in:

  • Product recalls and withdrawals from the market

  • Financial penalties and fines

  • Damage to reputation and brand image

  • Loss of customer trust and loyalty

    • Industries and Sectors Requiring AOAC 2003.01 Testing

    AOAC 2003.01 testing is required for various industries, including:

  • Food manufacturers

  • Grain traders

  • Feed producers

  • Regulatory agencies

    • Standard Requirements Summary

    Standard Description

    --- ---

    AOAC 2003.01 Deoxynivalenol (DON) - Method using liquid chromatography with mass spectrometry detection (LC-MS/MS)

    ISO 21591:2014 Mycotoxins in foodstuffs - Determination of deoxynivalenol (DON) and zearalenone (ZEN) by liquid chromatography with tandem mass spectrometry (LC-MS/MS)

    Standard Requirements Summary

    AOAC 2003.01 testing is a critical component of ensuring the safety and quality of corn-based products.

    Next Section: Standard Compliance Requirements

    The next section will provide detailed information on standard compliance requirements for AOAC 2003.01 testing, including regulatory requirements, testing frequencies, and sampling procedures.

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