AOAC 2005.09 Determination of Zearalenone in Maize

AOAC 2005.09 Determination of Zearalenone in Maize: Eurolabs Laboratory Testing Service

The AOAC 2005.09 method for the determination of zearalenone in maize is a widely recognized and accepted standard for laboratory testing. This method is part of the Association of Official Analytical Chemists (AOAC) Internationals portfolio of standards, which are designed to ensure the accuracy and reliability of analytical results.

The AOAC 2005.09 method is based on high-performance liquid chromatography with fluorescence detection (HPLC-FLD). The test involves extracting zearalenone from maize samples using a mixture of acetonitrile and water, followed by centrifugation and filtration. The extracted sample is then injected into the HPLC system, where the zearalenone content is determined using fluorescence detection.

The AOAC 2005.09 method has been validated through interlaboratory studies and has been shown to be reliable and accurate for determining zearalenone in maize. This method has been adopted by many regulatory agencies around the world as a standard for testing maize samples for zearalenone.

International and National Standards

The AOAC 2005.09 method is based on international standards, including:

These standards ensure that laboratory testing is conducted using consistent and reliable methods, which helps to build confidence in the results.

Standard Development Organizations

The AOAC International is a non-profit organization dedicated to developing and promoting standard methods for analytical chemistry. The AOAC International works closely with other standard development organizations, such as ISO, ASTM, and EN, to ensure that standards are consistent and comparable across different regions and industries.

Evolution of Standards

Standards evolve over time to reflect advances in technology, changes in regulatory requirements, or the need for more accurate or reliable testing methods. The AOAC International regularly reviews and updates its standards to ensure they remain relevant and effective.

Standard Numbers and Scope

The AOAC 2005.09 method has the following standard number:

The scope of this standard is to provide a reliable and accurate method for determining zearalenone in maize samples.

Industry-Specific Examples and Case Studies

The AOAC 2005.09 method has been widely adopted by the food industry, particularly in the production of maize-based products such as cornmeal, cornflakes, and animal feed. This standard ensures that these products are safe for human consumption and do not contain high levels of zearalenone.

Standard Compliance Requirements

Regulatory agencies around the world require compliance with international standards, including the AOAC 2005.09 method, to ensure the safety and quality of food products. Industry stakeholders must adhere to these standards to maintain their market position and reputation.

The AOAC 2005.09 method involves a series of steps that ensure accurate and reliable results:

1. Sample Preparation

The maize sample is ground into a fine powder using a laboratory mill or grinder. The powdered sample is then mixed with acetonitrile and water to extract zearalenone.

2. Extraction

The extracted sample is centrifuged at 4,000 rpm for 10 minutes to separate the liquid from the solid particles. The supernatant is filtered using a 0.45-μm filter membrane.

3. Injections

A volume of 20 μL of the filtered extract is injected into the HPLC system.

HPLC System

The HPLC system consists of:

The fluorescence detector is set at a wavelength of 274 nm for excitation and 360 nm for emission.

Measurement and Analysis Methods

The zearalenone content in the sample is determined using the HPLC system. The peak area of the zearalenone chromatogram is compared to a standard curve generated from a series of zearalenone standards.

Quality Control Measures

Eurolabs laboratory testing service adheres to strict quality control measures, including:

These measures ensure that the results obtained are accurate and reliable.

The test report includes:

Eurolabs laboratory testing service also provides a detailed certificate of analysis for each sample tested.

Persuasive Summary

The AOAC 2005.09 method is an internationally recognized and accepted standard for determining zearalenone in maize samples. Eurolabs laboratory testing service adheres to strict quality control measures, ensuring accurate and reliable results. Our experienced analysts use state-of-the-art equipment, including HPLC-FLD systems, to provide precise determinations of zearalenone content.

Call to Action

Contact us today to learn more about our AOAC 2005.09 laboratory testing service for determining zearalenone in maize samples. Our expert analysts are ready to help you ensure the safety and quality of your products.

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