EUROLAB
aoac-200802-detection-of-fumonisins-in-corn-using-hplc-fld
Mycotoxin Testing AOAC 2000.02 Patulin Testing in Apple PureeAOAC 2000.03 Determination of Patulin in Apple JuiceAOAC 2000.05 Patulin Detection in Fermented BeveragesAOAC 2003.01 Testing for Deoxynivalenol in Corn-Based ProductsAOAC 2003.02 Measurement of Deoxynivalenol in Wheat FlourAOAC 2003.03 Quantitative Analysis of Mycotoxins in Animal FeedAOAC 2005.06 Determination of Fumonisins by Immunoaffinity and LCAOAC 2005.07 Quantification of Aflatoxins in Tree NutsAOAC 2005.08 Determination of Zearalenone in Animal FeedAOAC 2005.09 Determination of Zearalenone in MaizeAOAC 2007.01 Quantitative Determination of Zearalenone in CornAOAC 2008.01 Fumonisin Testing in Corn GritsAOAC 2008.02 Quantification of Fumonisin B1 and B2 in MaizeAOAC 2008.03 Multi-Residue Detection of Mycotoxins in Animal FeedAOAC 986.16 Testing of Total Aflatoxins in Corn Using FluorometryAOAC 991.31 Aflatoxin Screening in Feed IngredientsAOAC 991.31 Determination of Aflatoxin B1 in Peanut ButterAOAC 991.31 Testing for Total Aflatoxins in Peanuts and CornAOAC 994.08 Screening of Aflatoxins by TLCAOAC 994.09 Detection of Aflatoxins in AlmondsAOAC 998.03 Screening for Aflatoxins in Maize Using Test KitsAOAC 999.07 Detection of Aflatoxin B1 in RiceCEN/TR 16059 General Protocol for Mycotoxin Testing in Food ChainsEN 14123 Determination of Ochratoxin A in Wine by Immunoaffinity ColumnEN 15289 Determination of Ochratoxin A in Cocoa by LCEN 15662 Multimycotoxin Detection in Fruits and Vegetables Using LC-MSEN 15788 Determination of Deoxynivalenol in Baby FoodEN 15792 Detection of Mycotoxins in Tomato PureeEN 15891 Determination of Trichothecenes in Food Using LC-MSEN 15891 HT-2/T-2 Detection in Baby FoodsEN 15891 Measurement of HT-2 and T-2 Toxins in CerealsEN 15891 T-2 and HT-2 Toxin Detection in OatsEN 16571 Testing of Aflatoxin M1 in Milk Products by ELISAEN 16619 Detection of Mycotoxins in Edible OilsEN 17193 Determination of Aflatoxicol in Milk and DairyEN 17194 Determination of Citrinin in Red Yeast Rice Food SupplementsEN 17200 Determination of Mycotoxins in SpicesEN 17201 Determination of Alternaria Toxins in Tomato ProductsEN 17250 Determination of Ergot Alkaloids in Feed by LC-MSEN 17299 Testing of Modified Mycotoxins in Animal FeedEN 17461 Determination of Fusarenon-X in Cereals by LC-MSEN 17462 Rapid Testing of Citrinin in Red Fermented ProductsEN 17463 Screening of Ergot Alkaloids in GrainEN 17521 Mycotoxin Profiling in Complex Matrices Using LC-MS/MSEU 2006/401/EC Mycotoxin Control in Import CommoditiesEU Regulation 1881/2006 Compliance Testing for Mycotoxin Levels in FoodFAO Recommended Methods for Mycotoxin Sampling and DetectionFDA BAM Chapter 19 Mycotoxins Analysis ProtocolsFDA BAM Mycotoxin Analytical Methods for Regulatory MonitoringFDA CFR 21 Part 556 Testing for Mycotoxin Tolerances in Animal FeedISO 14501 Determination of Patulin in Fruit Juices by HPLCISO 15141 Determination of Deoxynivalenol in Cereals by GC-MSISO 16050 Determination of Aflatoxin B1, B2, G1, G2 in CerealsISO 16050 Determination of Aflatoxins in Foodstuffs Using HPLCISO 16050 Testing for Aflatoxins in Herbs and SpicesISO 17375 Determination of Ochratoxin A in Roasted Coffee by HPLCISO 18787 Guidelines for Mycotoxin Sampling in GrainsISO 18856 Determination of Ochratoxin A in Foodstuffs by LC-MS/MSISO 21527 Mold Enumeration for Mycotoxin-Prone FoodsISO 21527-1 Enumeration of Yeasts and Molds for Spoilage RiskISO 24276 Guidelines for Validation of Mycotoxin Detection MethodsISO 24276 Method Validation for Zearalenone DetectionJECFA Limits-Based Mycotoxin Testing in Dried FruitsJECFA Maximum Levels of Mycotoxins in Cereal GrainsJECFA Toxicity Testing for Aflatoxin M1 in Milk

AOAC 2008.02 Detection of Fumonisins in Corn Using HPLC-FLD Laboratory Testing Service Provided by Eurolab: A Comprehensive Guide

The AOAC 2008.02 method for detecting fumonisins in corn using high-performance liquid chromatography with fluorescence detection (HPLC-FLD) is a widely accepted and validated testing procedure. This method is governed by various international and national standards, including:

  • AOAC International Method 2008.02: Detection of Fumonisins in Corn Using HPLC-FLD

  • ISO 17025:2017: General Requirements for the Competence of Testing and Calibration Laboratories

  • EN 15894:2013: Fumonisins in Maize - Extraction, Separation and Determination by Liquid Chromatography with Fluorescence Detection (HPLC-FLD)

  • TSE 2009/1: Food Safety - Fumonisins in Corn

    • These standards ensure that the testing service provided by Eurolab meets the required quality and safety criteria. The AOAC International method is a widely accepted standard for detecting fumonisins in corn, while the ISO 17025:2017 standard ensures that laboratories meet the requirements for competence.

    Standard Development Organizations and Their Role

  • AOAC International: Develops and publishes methods and standards for testing and calibration

  • ISO (International Organization for Standardization): Develops and publishes international standards

  • EN (European Committee for Standardization): Develops and publishes European standards

  • TSE (Turkish Standards Institution): Develops and publishes Turkish national standards

    • These standard development organizations play a crucial role in ensuring that laboratory testing services meet the required quality and safety criteria.

    International and National Standards

    The AOAC 2008.02 method is widely accepted and used by laboratories around the world. The international and national standards mentioned above ensure that the testing service provided by Eurolab meets the required quality and safety criteria. Some of the key standards are:

  • ISO 17025:2017: General Requirements for the Competence of Testing and Calibration Laboratories

  • EN 15894:2013: Fumonisins in Maize - Extraction, Separation and Determination by Liquid Chromatography with Fluorescence Detection (HPLC-FLD)

  • TSE 2009/1: Food Safety - Fumonisins in Corn

    • Standard Compliance Requirements for Different Industries

    Different industries have varying standard compliance requirements. For example:

  • Food Industry: AOAC International Method 2008.02 and ISO 17025:2017

  • Pharmaceutical Industry: USP <621> and EP 5.1

  • Cosmetic Industry: ISO 13485:2016

    • Eurolabs laboratory testing service is designed to meet the standard compliance requirements of different industries.

    The AOAC 2008.02 method for detecting fumonisins in corn using HPLC-FLD is a widely accepted and validated testing procedure. The business and technical reasons for conducting this test are:

  • Food Safety: Fumonisins can cause foodborne illnesses, so its essential to detect them in corn

  • Regulatory Compliance: AOAC International Method 2008.02 and ISO 17025:2017 ensure compliance with regulatory requirements

  • Quality Assurance: Regular testing helps maintain product quality and safety

    • The consequences of not performing this test are:

  • Food Safety Risks: Fumonisins can cause foodborne illnesses, leading to customer complaints and reputational damage

  • Regulatory Non-Compliance: Failure to comply with regulatory requirements can result in fines and penalties

  • Quality Assurance Issues: Lack of regular testing can lead to product contamination and quality issues

    • The AOAC 2008.02 method for detecting fumonisins in corn using HPLC-FLD involves the following steps:

    1. Sample Preparation: Weigh and mix a representative sample of corn with an internal standard

    2. Extraction: Use a solvent to extract fumonisins from the corn sample

    3. Separation: Separate the extracted fumonisins using HPLC-FLD

    4. Detection: Detect fumonisins using fluorescence detection

    The testing equipment and instruments used are:

  • HPLC System: Shimadzu or Agilent

  • Fluorescence Detector: Shimadzu or Agilent

  • Sample Preparation Equipment: Vortex mixer, centrifuge, and pipettes

    • Quality Control Measures

    Eurolab takes quality control measures to ensure the accuracy and reliability of test results. These measures include:

  • Calibration: Regular calibration of HPLC-FLD equipment

  • Quality Control Samples: Use of quality control samples for testing and validation

  • Data Analysis: Data analysis using statistical software (e.g., Excel, R)

    • Test Results and Reporting

    The AOAC 2008.02 method provides accurate and reliable results for detecting fumonisins in corn using HPLC-FLD. The test report will include:

  • Sample Identification: Sample name, code, and date of analysis

  • Test Results: Concentration of fumonisins in the sample (mg/kg)

  • Method Detection Limit (MDL): Minimum concentration detectable by the method

    • The AOAC 2008.02 method for detecting fumonisins in corn using HPLC-FLD is a widely accepted and validated testing procedure. Eurolabs laboratory testing service is designed to meet the standard compliance requirements of different industries.

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    Next Section: Case Studies and Success Stories

    This comprehensive guide has provided an overview of the AOAC 2008.02 method for detecting fumonisins in corn using HPLC-FLD, including standard-related information, standard development organizations, international and national standards, and standard compliance requirements for different industries.

    In the next section, we will provide case studies and success stories demonstrating Eurolabs expertise in laboratory testing services for AOAC 2008.02 method.

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