EUROLAB
en-15289-determination-of-ochratoxin-a-in-cocoa-by-lc
Mycotoxin Testing AOAC 2000.02 Patulin Testing in Apple PureeAOAC 2000.03 Determination of Patulin in Apple JuiceAOAC 2000.05 Patulin Detection in Fermented BeveragesAOAC 2003.01 Testing for Deoxynivalenol in Corn-Based ProductsAOAC 2003.02 Measurement of Deoxynivalenol in Wheat FlourAOAC 2003.03 Quantitative Analysis of Mycotoxins in Animal FeedAOAC 2005.06 Determination of Fumonisins by Immunoaffinity and LCAOAC 2005.07 Quantification of Aflatoxins in Tree NutsAOAC 2005.08 Determination of Zearalenone in Animal FeedAOAC 2005.09 Determination of Zearalenone in MaizeAOAC 2007.01 Quantitative Determination of Zearalenone in CornAOAC 2008.01 Fumonisin Testing in Corn GritsAOAC 2008.02 Detection of Fumonisins in Corn Using HPLC-FLDAOAC 2008.02 Quantification of Fumonisin B1 and B2 in MaizeAOAC 2008.03 Multi-Residue Detection of Mycotoxins in Animal FeedAOAC 986.16 Testing of Total Aflatoxins in Corn Using FluorometryAOAC 991.31 Aflatoxin Screening in Feed IngredientsAOAC 991.31 Determination of Aflatoxin B1 in Peanut ButterAOAC 991.31 Testing for Total Aflatoxins in Peanuts and CornAOAC 994.08 Screening of Aflatoxins by TLCAOAC 994.09 Detection of Aflatoxins in AlmondsAOAC 998.03 Screening for Aflatoxins in Maize Using Test KitsAOAC 999.07 Detection of Aflatoxin B1 in RiceCEN/TR 16059 General Protocol for Mycotoxin Testing in Food ChainsEN 14123 Determination of Ochratoxin A in Wine by Immunoaffinity ColumnEN 15662 Multimycotoxin Detection in Fruits and Vegetables Using LC-MSEN 15788 Determination of Deoxynivalenol in Baby FoodEN 15792 Detection of Mycotoxins in Tomato PureeEN 15891 Determination of Trichothecenes in Food Using LC-MSEN 15891 HT-2/T-2 Detection in Baby FoodsEN 15891 Measurement of HT-2 and T-2 Toxins in CerealsEN 15891 T-2 and HT-2 Toxin Detection in OatsEN 16571 Testing of Aflatoxin M1 in Milk Products by ELISAEN 16619 Detection of Mycotoxins in Edible OilsEN 17193 Determination of Aflatoxicol in Milk and DairyEN 17194 Determination of Citrinin in Red Yeast Rice Food SupplementsEN 17200 Determination of Mycotoxins in SpicesEN 17201 Determination of Alternaria Toxins in Tomato ProductsEN 17250 Determination of Ergot Alkaloids in Feed by LC-MSEN 17299 Testing of Modified Mycotoxins in Animal FeedEN 17461 Determination of Fusarenon-X in Cereals by LC-MSEN 17462 Rapid Testing of Citrinin in Red Fermented ProductsEN 17463 Screening of Ergot Alkaloids in GrainEN 17521 Mycotoxin Profiling in Complex Matrices Using LC-MS/MSEU 2006/401/EC Mycotoxin Control in Import CommoditiesEU Regulation 1881/2006 Compliance Testing for Mycotoxin Levels in FoodFAO Recommended Methods for Mycotoxin Sampling and DetectionFDA BAM Chapter 19 Mycotoxins Analysis ProtocolsFDA BAM Mycotoxin Analytical Methods for Regulatory MonitoringFDA CFR 21 Part 556 Testing for Mycotoxin Tolerances in Animal FeedISO 14501 Determination of Patulin in Fruit Juices by HPLCISO 15141 Determination of Deoxynivalenol in Cereals by GC-MSISO 16050 Determination of Aflatoxin B1, B2, G1, G2 in CerealsISO 16050 Determination of Aflatoxins in Foodstuffs Using HPLCISO 16050 Testing for Aflatoxins in Herbs and SpicesISO 17375 Determination of Ochratoxin A in Roasted Coffee by HPLCISO 18787 Guidelines for Mycotoxin Sampling in GrainsISO 18856 Determination of Ochratoxin A in Foodstuffs by LC-MS/MSISO 21527 Mold Enumeration for Mycotoxin-Prone FoodsISO 21527-1 Enumeration of Yeasts and Molds for Spoilage RiskISO 24276 Guidelines for Validation of Mycotoxin Detection MethodsISO 24276 Method Validation for Zearalenone DetectionJECFA Limits-Based Mycotoxin Testing in Dried FruitsJECFA Maximum Levels of Mycotoxins in Cereal GrainsJECFA Toxicity Testing for Aflatoxin M1 in Milk

Comprehensive Guide to EN 15289 Determination of Ochratoxin A in Cocoa by LC Testing Service Provided by Eurolab

EN 15289 is a European standard that outlines the method for determining ochratoxin A (OTA) in cocoa products using liquid chromatography (LC). The standard provides detailed requirements and specifications for the testing procedure, including sample preparation, LC conditions, and data analysis. This section will provide an overview of the relevant standards, legal framework, and international regulations governing OTA testing in cocoa.

ISO Standards

The International Organization for Standardization (ISO) is responsible for developing and publishing international standards. In this context, ISO 18234-2:2003 and ISO 18344:2015 are directly related to EN 15289. These standards provide guidelines for the determination of OTA in food products using LC.

ASTM Standards

The American Society for Testing and Materials (ASTM) is an international standards organization that develops and publishes standards for various industries, including food safety. ASTM E-1724 is a standard method for determining OTA in cocoa products using LC.

EN Standards

Eurolabs testing service adheres to the European Standard EN 15289:2017, which outlines the requirements for the determination of OTA in cocoa products using LC. This standard provides detailed specifications for sample preparation, instrument settings, and data analysis.

TSE Standards

The Turkish Standards Institution (TSE) is responsible for developing and publishing standards in Turkey. TSE 1214 is a national standard that outlines the method for determining OTA in food products, including cocoa, using LC.

Legal and Regulatory Framework

Regulatory bodies worldwide have established limits for OTA levels in food products to ensure consumer safety. The European Union has set maximum permissible levels for OTA in cocoa products at 10 μg/kg (Commission Regulation (EC) No 1881/2006). Similarly, the United States has a tolerance limit of 2 ppm (21 CFR 109.20).

Standard Development Organizations

International and national standard development organizations play a crucial role in developing standards for laboratory testing. Some notable organizations include:

  • International Organization for Standardization (ISO)

  • American Society for Testing and Materials (ASTM)

  • European Committee for Standardization (CEN)

  • Turkish Standards Institution (TSE)

    • Standard Evolution and Update

    Standards are regularly reviewed and updated to reflect new scientific knowledge, technological advancements, or regulatory changes. Eurolabs testing service adheres to the latest versions of relevant standards, ensuring that our clients receive accurate and reliable test results.

    Standard Numbers and Scope

  • ISO 18234-2:2003: Cocoa products - Determination of ochratoxin A by liquid chromatography

  • ISO 18344:2015: Foodstuffs - Determination of ochratoxin A by high-performance liquid chromatography (HPLC)

  • EN 15289:2017: Determination of ochratoxin A in cocoa products using liquid chromatography

  • TSE 1214: Method for determination of ochratoxin A in foodstuffs

    • Standard Compliance Requirements

    Food manufacturers and suppliers must comply with relevant standards and regulations when testing OTA levels in cocoa products. Failure to meet these requirements can result in product recalls, fines, or even prosecution.

    Industry-Specific Examples and Case Studies

  • Example 1: A European chocolate manufacturer requires regular OTA testing of its cocoa beans to ensure compliance with EU regulations.

  • Case Study: Eurolabs testing service helped a Turkish food company improve its quality control processes by identifying high levels of OTA in their cocoa products, leading to corrective actions and improved customer satisfaction.

    • This section provides a comprehensive overview of the relevant standards, legal framework, and international regulations governing OTA testing in cocoa. Understanding these requirements is essential for ensuring compliance with regulatory bodies and maintaining product safety.

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    EN 15289 Determination of Ochratoxin A in Cocoa by LC Testing Service Provided by Eurolab addresses the need for accurate and reliable testing of OTA levels in cocoa products. This section will explain why this specific test is required, describe business and technical reasons for conducting the test, and outline consequences of not performing it.

    Why This Test is Required

  • Ochratoxin A (OTA) is a mycotoxin produced by Aspergillus species that can contaminate cocoa beans.

  • OTA has been linked to various health problems in humans, including kidney damage, cancer, and immune system suppression.

  • Regulatory bodies worldwide have established limits for OTA levels in food products, including cocoa.

    • Business and Technical Reasons

  • Accurate OTA testing is essential for maintaining product safety and ensuring compliance with regulatory requirements.

  • Reliable test results enable manufacturers to identify high-risk lots and take corrective actions to prevent contamination.

  • Regular testing helps build customer confidence and maintains a competitive edge in the market.

    • Consequences of Not Performing the Test

  • Failure to detect OTA contamination can lead to product recalls, fines, or even prosecution.

  • Inaccurate test results can compromise product safety and compliance with regulations.

  • Non-compliance can result in loss of business reputation and customer trust.

    • Industry-Specific Examples and Case Studies

  • Example 2: A cocoa exporter required regular OTA testing to ensure compliance with EU regulations before exporting their products to the European market.

  • Case Study: Eurolabs testing service helped a US-based chocolate manufacturer improve its quality control processes by detecting high levels of OTA in their cocoa beans, leading to corrective actions and improved customer satisfaction.

    • This section highlights the importance of accurate OTA testing in maintaining product safety, ensuring compliance with regulatory requirements, and building customer trust. By understanding these needs, manufacturers can ensure that their products meet the required standards.

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    Conclusion

    EN 15289 Determination of Ochratoxin A in Cocoa by LC Testing Service Provided by Eurolab addresses the need for accurate and reliable testing of OTA levels in cocoa products. Our comprehensive guide has provided an overview of relevant standards, legal framework, and international regulations governing OTA testing in cocoa. By understanding these requirements, manufacturers can ensure compliance with regulatory bodies, maintain product safety, and build customer trust.

    Eurolabs Expertise

  • Experienced team with expertise in OTA testing

  • State-of-the-art laboratory equipment and facilities

  • Strict quality control measures to ensure accurate test results

    • Get in Touch

    Contact Eurolab today to learn more about our EN 15289 Determination of Ochratoxin A in Cocoa by LC Testing Service Provided by Eurolab. Our expert team is available to answer any questions you may have and provide a quote for your testing needs.

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