EUROLAB
en-15891-measurement-of-ht-2-and-t-2-toxins-in-cereals
Mycotoxin Testing AOAC 2000.02 Patulin Testing in Apple PureeAOAC 2000.03 Determination of Patulin in Apple JuiceAOAC 2000.05 Patulin Detection in Fermented BeveragesAOAC 2003.01 Testing for Deoxynivalenol in Corn-Based ProductsAOAC 2003.02 Measurement of Deoxynivalenol in Wheat FlourAOAC 2003.03 Quantitative Analysis of Mycotoxins in Animal FeedAOAC 2005.06 Determination of Fumonisins by Immunoaffinity and LCAOAC 2005.07 Quantification of Aflatoxins in Tree NutsAOAC 2005.08 Determination of Zearalenone in Animal FeedAOAC 2005.09 Determination of Zearalenone in MaizeAOAC 2007.01 Quantitative Determination of Zearalenone in CornAOAC 2008.01 Fumonisin Testing in Corn GritsAOAC 2008.02 Detection of Fumonisins in Corn Using HPLC-FLDAOAC 2008.02 Quantification of Fumonisin B1 and B2 in MaizeAOAC 2008.03 Multi-Residue Detection of Mycotoxins in Animal FeedAOAC 986.16 Testing of Total Aflatoxins in Corn Using FluorometryAOAC 991.31 Aflatoxin Screening in Feed IngredientsAOAC 991.31 Determination of Aflatoxin B1 in Peanut ButterAOAC 991.31 Testing for Total Aflatoxins in Peanuts and CornAOAC 994.08 Screening of Aflatoxins by TLCAOAC 994.09 Detection of Aflatoxins in AlmondsAOAC 998.03 Screening for Aflatoxins in Maize Using Test KitsAOAC 999.07 Detection of Aflatoxin B1 in RiceCEN/TR 16059 General Protocol for Mycotoxin Testing in Food ChainsEN 14123 Determination of Ochratoxin A in Wine by Immunoaffinity ColumnEN 15289 Determination of Ochratoxin A in Cocoa by LCEN 15662 Multimycotoxin Detection in Fruits and Vegetables Using LC-MSEN 15788 Determination of Deoxynivalenol in Baby FoodEN 15792 Detection of Mycotoxins in Tomato PureeEN 15891 Determination of Trichothecenes in Food Using LC-MSEN 15891 HT-2/T-2 Detection in Baby FoodsEN 15891 T-2 and HT-2 Toxin Detection in OatsEN 16571 Testing of Aflatoxin M1 in Milk Products by ELISAEN 16619 Detection of Mycotoxins in Edible OilsEN 17193 Determination of Aflatoxicol in Milk and DairyEN 17194 Determination of Citrinin in Red Yeast Rice Food SupplementsEN 17200 Determination of Mycotoxins in SpicesEN 17201 Determination of Alternaria Toxins in Tomato ProductsEN 17250 Determination of Ergot Alkaloids in Feed by LC-MSEN 17299 Testing of Modified Mycotoxins in Animal FeedEN 17461 Determination of Fusarenon-X in Cereals by LC-MSEN 17462 Rapid Testing of Citrinin in Red Fermented ProductsEN 17463 Screening of Ergot Alkaloids in GrainEN 17521 Mycotoxin Profiling in Complex Matrices Using LC-MS/MSEU 2006/401/EC Mycotoxin Control in Import CommoditiesEU Regulation 1881/2006 Compliance Testing for Mycotoxin Levels in FoodFAO Recommended Methods for Mycotoxin Sampling and DetectionFDA BAM Chapter 19 Mycotoxins Analysis ProtocolsFDA BAM Mycotoxin Analytical Methods for Regulatory MonitoringFDA CFR 21 Part 556 Testing for Mycotoxin Tolerances in Animal FeedISO 14501 Determination of Patulin in Fruit Juices by HPLCISO 15141 Determination of Deoxynivalenol in Cereals by GC-MSISO 16050 Determination of Aflatoxin B1, B2, G1, G2 in CerealsISO 16050 Determination of Aflatoxins in Foodstuffs Using HPLCISO 16050 Testing for Aflatoxins in Herbs and SpicesISO 17375 Determination of Ochratoxin A in Roasted Coffee by HPLCISO 18787 Guidelines for Mycotoxin Sampling in GrainsISO 18856 Determination of Ochratoxin A in Foodstuffs by LC-MS/MSISO 21527 Mold Enumeration for Mycotoxin-Prone FoodsISO 21527-1 Enumeration of Yeasts and Molds for Spoilage RiskISO 24276 Guidelines for Validation of Mycotoxin Detection MethodsISO 24276 Method Validation for Zearalenone DetectionJECFA Limits-Based Mycotoxin Testing in Dried FruitsJECFA Maximum Levels of Mycotoxins in Cereal GrainsJECFA Toxicity Testing for Aflatoxin M1 in Milk

EN 15891 Measurement of HT-2 and T-2 Toxins in Cereals Laboratory Testing Service Provided by Eurolab: A Comprehensive Guide

The EN 15891 standard is a European standard that governs the measurement of HT-2 and T-2 toxins in cereals. This standard is part of the larger family of standards related to food safety, specifically those dealing with mycotoxins.

ISO Standards

  • ISO/TS 23781:2015 - Mycotoxin - Measurement of HT-2 and T-2 toxins in cereals
  • ISO/TS 23816:2016 - Mycotoxin - Determination of aflatoxins B1, B2, G1, G2, M1, M2, F1, and F2 in peanut butter
  • EN Standards

  • EN 15891:2020 - Measurement of HT-2 and T-2 toxins in cereals
  • EN 15788:2019 - Determination of aflatoxins B1, B2, G1, and G2 in peanuts by reversed-phase high-performance liquid chromatography (HPLC)
  • TSE Standards

  • TS EN ISO/TS 23781:2017 - Mycotoxin - Measurement of HT-2 and T-2 toxins in cereals
  • TS EN ISO/TS 23816:2018 - Mycotoxin - Determination of aflatoxins B1, B2, G1, G2, M1, M2, F1, and F2 in peanut butter
  • ASTM Standards

  • ASTM E2557-12 - Standard Practice for Sampling and Analysis of Aflatoxins in Groundnuts
  • ASTM E2736-13 - Standard Guide for Sampling and Analysis of Mycotoxins in Cereals
  • Standard Development Organizations

    The standard development organizations responsible for the creation, maintenance, and revision of these standards include:

  • International Organization for Standardization (ISO)
  • European Committee for Standardization (CEN)
  • Turkish Standards Institution (TSE)
  • American Society for Testing and Materials (ASTM)
  • These organizations play a crucial role in ensuring that standards are developed, maintained, and updated to reflect the latest scientific knowledge and technological advancements.

    Evolution of Standards

    Standards evolve as new research emerges and technologies improve. For example, the EN 15891 standard has undergone several revisions since its initial publication in 2009. Each revision incorporates the latest scientific findings and methods for measuring HT-2 and T-2 toxins in cereals.

    Standard Numbers and Scope

    The following are some of the relevant standard numbers and their scopes:

  • ISO/TS 23781:2015 - Mycotoxin - Measurement of HT-2 and T-2 toxins in cereals
  • Scope: This standard provides a method for the measurement of HT-2 and T-2 toxins in cereals using liquid chromatography-tandem mass spectrometry (LC-MS/MS).

  • EN 15891:2020 - Measurement of HT-2 and T-2 toxins in cereals
  • Scope: This standard provides a method for the measurement of HT-2 and T-2 toxins in cereals using gas chromatography-mass spectrometry (GC-MS).

    Standard Compliance Requirements

    Compliance with these standards is required by various industries, including food manufacturers, regulatory agencies, and certifying bodies. Failure to comply can result in product recalls, fines, or other penalties.

    The EN 15891 standard is necessary due to the potential health risks associated with HT-2 and T-2 toxins in cereals. These toxins are produced by certain species of fungi, such as Fusarium graminearum, which can contaminate crops during growth or storage.

    Business and Technical Reasons

    Conducting EN 15891 testing is essential for several reasons:

  • Ensures product safety: HT-2 and T-2 toxins have been linked to various health issues, including cancer, kidney disease, and neurological problems.
  • Complies with regulations: Regulatory agencies worldwide require food manufacturers to test their products for mycotoxins.
  • Enhances customer confidence: Testing can help build trust with consumers by demonstrating a commitment to product safety.
  • Consequences of Not Performing the Test

    Failure to conduct EN 15891 testing can result in:

  • Product recalls
  • Fines and penalties from regulatory agencies
  • Damage to brand reputation
  • Loss of market share
  • Industries and Sectors

    The following industries and sectors require EN 15891 testing:

  • Food manufacturers: Bakeries, breweries, dairy processors, and other food companies that use cereals in their products.
  • Regulatory agencies: Government agencies responsible for ensuring product safety, such as the US FDA and the European Commissions Health and Consumers Directorate-General.
  • Certifying bodies: Organizations that verify compliance with standards, such as NSF International and SGS.
  • Standard Requirements

    To comply with EN 15891, laboratories must:

  • Use a validated method for measuring HT-2 and T-2 toxins
  • Follow specific guidelines for sampling, storage, and handling of cereal samples
  • Report results in accordance with established protocols
  • Standard Specifications

    The following are some of the key specifications for EN 15891 testing:

  • Method: Liquid chromatography-tandem mass spectrometry (LC-MS/MS) or gas chromatography-mass spectrometry (GC-MS)
  • Sampling: Random sampling from a representative population
  • Storage and handling: Samples must be stored in a secure, temperature-controlled environment to prevent contamination
  • Standard Implementation

    To implement EN 15891 testing, laboratories should:

  • Familiarize themselves with the standards requirements and specifications
  • Validate their methods using certified reference materials (CRMs)
  • Develop a quality control program to ensure accurate results
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