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jecfa-toxicity-testing-for-aflatoxin-m1-in-milk
Mycotoxin Testing AOAC 2000.02 Patulin Testing in Apple PureeAOAC 2000.03 Determination of Patulin in Apple JuiceAOAC 2000.05 Patulin Detection in Fermented BeveragesAOAC 2003.01 Testing for Deoxynivalenol in Corn-Based ProductsAOAC 2003.02 Measurement of Deoxynivalenol in Wheat FlourAOAC 2003.03 Quantitative Analysis of Mycotoxins in Animal FeedAOAC 2005.06 Determination of Fumonisins by Immunoaffinity and LCAOAC 2005.07 Quantification of Aflatoxins in Tree NutsAOAC 2005.08 Determination of Zearalenone in Animal FeedAOAC 2005.09 Determination of Zearalenone in MaizeAOAC 2007.01 Quantitative Determination of Zearalenone in CornAOAC 2008.01 Fumonisin Testing in Corn GritsAOAC 2008.02 Detection of Fumonisins in Corn Using HPLC-FLDAOAC 2008.02 Quantification of Fumonisin B1 and B2 in MaizeAOAC 2008.03 Multi-Residue Detection of Mycotoxins in Animal FeedAOAC 986.16 Testing of Total Aflatoxins in Corn Using FluorometryAOAC 991.31 Aflatoxin Screening in Feed IngredientsAOAC 991.31 Determination of Aflatoxin B1 in Peanut ButterAOAC 991.31 Testing for Total Aflatoxins in Peanuts and CornAOAC 994.08 Screening of Aflatoxins by TLCAOAC 994.09 Detection of Aflatoxins in AlmondsAOAC 998.03 Screening for Aflatoxins in Maize Using Test KitsAOAC 999.07 Detection of Aflatoxin B1 in RiceCEN/TR 16059 General Protocol for Mycotoxin Testing in Food ChainsEN 14123 Determination of Ochratoxin A in Wine by Immunoaffinity ColumnEN 15289 Determination of Ochratoxin A in Cocoa by LCEN 15662 Multimycotoxin Detection in Fruits and Vegetables Using LC-MSEN 15788 Determination of Deoxynivalenol in Baby FoodEN 15792 Detection of Mycotoxins in Tomato PureeEN 15891 Determination of Trichothecenes in Food Using LC-MSEN 15891 HT-2/T-2 Detection in Baby FoodsEN 15891 Measurement of HT-2 and T-2 Toxins in CerealsEN 15891 T-2 and HT-2 Toxin Detection in OatsEN 16571 Testing of Aflatoxin M1 in Milk Products by ELISAEN 16619 Detection of Mycotoxins in Edible OilsEN 17193 Determination of Aflatoxicol in Milk and DairyEN 17194 Determination of Citrinin in Red Yeast Rice Food SupplementsEN 17200 Determination of Mycotoxins in SpicesEN 17201 Determination of Alternaria Toxins in Tomato ProductsEN 17250 Determination of Ergot Alkaloids in Feed by LC-MSEN 17299 Testing of Modified Mycotoxins in Animal FeedEN 17461 Determination of Fusarenon-X in Cereals by LC-MSEN 17462 Rapid Testing of Citrinin in Red Fermented ProductsEN 17463 Screening of Ergot Alkaloids in GrainEN 17521 Mycotoxin Profiling in Complex Matrices Using LC-MS/MSEU 2006/401/EC Mycotoxin Control in Import CommoditiesEU Regulation 1881/2006 Compliance Testing for Mycotoxin Levels in FoodFAO Recommended Methods for Mycotoxin Sampling and DetectionFDA BAM Chapter 19 Mycotoxins Analysis ProtocolsFDA BAM Mycotoxin Analytical Methods for Regulatory MonitoringFDA CFR 21 Part 556 Testing for Mycotoxin Tolerances in Animal FeedISO 14501 Determination of Patulin in Fruit Juices by HPLCISO 15141 Determination of Deoxynivalenol in Cereals by GC-MSISO 16050 Determination of Aflatoxin B1, B2, G1, G2 in CerealsISO 16050 Determination of Aflatoxins in Foodstuffs Using HPLCISO 16050 Testing for Aflatoxins in Herbs and SpicesISO 17375 Determination of Ochratoxin A in Roasted Coffee by HPLCISO 18787 Guidelines for Mycotoxin Sampling in GrainsISO 18856 Determination of Ochratoxin A in Foodstuffs by LC-MS/MSISO 21527 Mold Enumeration for Mycotoxin-Prone FoodsISO 21527-1 Enumeration of Yeasts and Molds for Spoilage RiskISO 24276 Guidelines for Validation of Mycotoxin Detection MethodsISO 24276 Method Validation for Zearalenone DetectionJECFA Limits-Based Mycotoxin Testing in Dried FruitsJECFA Maximum Levels of Mycotoxins in Cereal Grains

JECFA Toxicity Testing for Aflatoxin M1 in Milk: Eurolabs Laboratory Testing Service

Aflatoxin M1 (AFM1) is a toxic metabolite produced by the Aspergillus flavus fungus, which contaminates milk and dairy products. The Joint FAO/WHO Expert Committee on Food Additives (JECFA) has established a tolerable daily intake for AFM1 to ensure consumer safety. Eurolabs JECFA toxicity testing service for Aflatoxin M1 in milk is designed to monitor and regulate the levels of this toxin in milk and dairy products.

Relevant Standards:

  • ISO 16140-2 (2016): Microbiology of food and animal feeding stuffs -- Horizontal method for the detection of AFM1
  • ASTM E2659-16: Standard Practice for Sampling Dairy Products for Aflatoxin M1 Analysis
  • EN 14264 (2005): Milk and milk products -- Determination of aflatoxins B1, B2, G1, G2, M1, and M2
  • TSE (Turkish Standards Institution) TS 1350:2009: Aflatoxin M1 in milk and dairy products
  • Legal and Regulatory Framework:

    The European Union has set maximum limits for AFM1 in milk and dairy products through Regulation (EC) No 1881/2006. The US FDA has established a tolerance of 20 ppb for AFM1 in milk and dairy products. Other countries, such as Canada, Australia, and New Zealand, have similar regulations.

    International and National Standards:

    JECFA toxicity testing for Aflatoxin M1 in milk is governed by international standards, including those mentioned above. Eurolabs laboratory follows these standards to ensure compliance with regulatory requirements.

    Standard Development Organizations:

    Standards development organizations, such as ISO, ASTM, and EN, play a crucial role in establishing and maintaining standards for laboratory testing. These organizations work together with industry stakeholders to develop and update standards as needed.

    How Standards Evolve and Get Updated:

    Standards evolve through a collaborative process between experts from various fields, including industry, academia, and regulatory agencies. Updates are made to reflect advances in technology, changes in consumer preferences, or new scientific discoveries.

    Standard Numbers and Scope:

    Some relevant standard numbers and their scope are:

  • ISO 16140-2 (2016): Horizontal method for the detection of AFM1
  • ASTM E2659-16: Standard Practice for Sampling Dairy Products for Aflatoxin M1 Analysis
  • EN 14264 (2005): Determination of aflatoxins B1, B2, G1, G2, M1, and M2
  • Standard Compliance Requirements:

    Compliance with standards is essential for industries that require JECFA toxicity testing for Aflatoxin M1 in milk. These requirements vary by industry and country.

    Industry-Specific Examples and Case Studies:

  • Dairy companies must comply with EU regulations to export products to the European market.
  • Food manufacturers may need to test their dairy ingredients for AFM1 levels to ensure compliance with regulatory requirements.
  • Regulatory agencies, such as the FDA, require testing of milk and dairy products to enforce consumer safety standards.
  • Statistical Data and Research Findings:

    Studies have shown that exposure to high levels of AFM1 can lead to liver damage and cancer. The European Union has established a maximum limit for AFM1 in milk and dairy products to protect consumers from this risk.

    Eurolabs JECFA toxicity testing service for Aflatoxin M1 in milk involves the following steps:

    Sample Preparation:

  • Collect milk samples from dairy farms or processing plants
  • Transport samples to Eurolabs laboratory for analysis
  • Prepare samples according to standard protocols (e.g., ISO 16140-2)
  • Testing Equipment and Instruments:

    Eurolab uses state-of-the-art equipment, including:

  • High-performance liquid chromatography (HPLC) system
  • Mass spectrometry (MS) instrument
  • Automatic sampler
  • Testing Environment Requirements:

    The testing environment must meet specific requirements, including temperature, humidity, and pressure control.

    Sample Preparation Procedures:

    Eurolabs laboratory follows standard protocols for sample preparation, which includes:

  • Centrifugation of milk samples to separate cream from skimmed milk
  • Filtration of samples through a 0.45-μm filter
  • Testing Parameters and Conditions:

    The testing parameters and conditions include:

  • Detection limit: 1 ppb
  • Quantitation limit: 2 ppb
  • Sample volume: 10 mL
  • Test Methodology:

    Eurolabs laboratory uses the following test methodology:

  • HPLC-MS/MS analysis of AFM1 in milk samples
  • Calibration and validation of analytical methods according to standard protocols (e.g., ISO 16140-2)
  • Turnaround Time:

    Results are typically available within 24 hours.

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