EUROLAB
en-15891-determination-of-trichothecenes-in-food-using-lc-ms
Mycotoxin Testing AOAC 2000.02 Patulin Testing in Apple PureeAOAC 2000.03 Determination of Patulin in Apple JuiceAOAC 2000.05 Patulin Detection in Fermented BeveragesAOAC 2003.01 Testing for Deoxynivalenol in Corn-Based ProductsAOAC 2003.02 Measurement of Deoxynivalenol in Wheat FlourAOAC 2003.03 Quantitative Analysis of Mycotoxins in Animal FeedAOAC 2005.06 Determination of Fumonisins by Immunoaffinity and LCAOAC 2005.07 Quantification of Aflatoxins in Tree NutsAOAC 2005.08 Determination of Zearalenone in Animal FeedAOAC 2005.09 Determination of Zearalenone in MaizeAOAC 2007.01 Quantitative Determination of Zearalenone in CornAOAC 2008.01 Fumonisin Testing in Corn GritsAOAC 2008.02 Detection of Fumonisins in Corn Using HPLC-FLDAOAC 2008.02 Quantification of Fumonisin B1 and B2 in MaizeAOAC 2008.03 Multi-Residue Detection of Mycotoxins in Animal FeedAOAC 986.16 Testing of Total Aflatoxins in Corn Using FluorometryAOAC 991.31 Aflatoxin Screening in Feed IngredientsAOAC 991.31 Determination of Aflatoxin B1 in Peanut ButterAOAC 991.31 Testing for Total Aflatoxins in Peanuts and CornAOAC 994.08 Screening of Aflatoxins by TLCAOAC 994.09 Detection of Aflatoxins in AlmondsAOAC 998.03 Screening for Aflatoxins in Maize Using Test KitsAOAC 999.07 Detection of Aflatoxin B1 in RiceCEN/TR 16059 General Protocol for Mycotoxin Testing in Food ChainsEN 14123 Determination of Ochratoxin A in Wine by Immunoaffinity ColumnEN 15289 Determination of Ochratoxin A in Cocoa by LCEN 15662 Multimycotoxin Detection in Fruits and Vegetables Using LC-MSEN 15788 Determination of Deoxynivalenol in Baby FoodEN 15792 Detection of Mycotoxins in Tomato PureeEN 15891 HT-2/T-2 Detection in Baby FoodsEN 15891 Measurement of HT-2 and T-2 Toxins in CerealsEN 15891 T-2 and HT-2 Toxin Detection in OatsEN 16571 Testing of Aflatoxin M1 in Milk Products by ELISAEN 16619 Detection of Mycotoxins in Edible OilsEN 17193 Determination of Aflatoxicol in Milk and DairyEN 17194 Determination of Citrinin in Red Yeast Rice Food SupplementsEN 17200 Determination of Mycotoxins in SpicesEN 17201 Determination of Alternaria Toxins in Tomato ProductsEN 17250 Determination of Ergot Alkaloids in Feed by LC-MSEN 17299 Testing of Modified Mycotoxins in Animal FeedEN 17461 Determination of Fusarenon-X in Cereals by LC-MSEN 17462 Rapid Testing of Citrinin in Red Fermented ProductsEN 17463 Screening of Ergot Alkaloids in GrainEN 17521 Mycotoxin Profiling in Complex Matrices Using LC-MS/MSEU 2006/401/EC Mycotoxin Control in Import CommoditiesEU Regulation 1881/2006 Compliance Testing for Mycotoxin Levels in FoodFAO Recommended Methods for Mycotoxin Sampling and DetectionFDA BAM Chapter 19 Mycotoxins Analysis ProtocolsFDA BAM Mycotoxin Analytical Methods for Regulatory MonitoringFDA CFR 21 Part 556 Testing for Mycotoxin Tolerances in Animal FeedISO 14501 Determination of Patulin in Fruit Juices by HPLCISO 15141 Determination of Deoxynivalenol in Cereals by GC-MSISO 16050 Determination of Aflatoxin B1, B2, G1, G2 in CerealsISO 16050 Determination of Aflatoxins in Foodstuffs Using HPLCISO 16050 Testing for Aflatoxins in Herbs and SpicesISO 17375 Determination of Ochratoxin A in Roasted Coffee by HPLCISO 18787 Guidelines for Mycotoxin Sampling in GrainsISO 18856 Determination of Ochratoxin A in Foodstuffs by LC-MS/MSISO 21527 Mold Enumeration for Mycotoxin-Prone FoodsISO 21527-1 Enumeration of Yeasts and Molds for Spoilage RiskISO 24276 Guidelines for Validation of Mycotoxin Detection MethodsISO 24276 Method Validation for Zearalenone DetectionJECFA Limits-Based Mycotoxin Testing in Dried FruitsJECFA Maximum Levels of Mycotoxins in Cereal GrainsJECFA Toxicity Testing for Aflatoxin M1 in Milk

Comprehensive Guide to EN 15891 Determination of Trichothecenes in Food Using LC-MS Testing Services Provided by Eurolab

EN 15891 is a European standard that outlines the requirements for determining trichothecenes in food using liquid chromatography-tandem mass spectrometry (LC-MS) laboratory testing. Trichothecenes are a group of mycotoxins produced by fungi, which can contaminate crops and pose a risk to human health.

Relevant Standards:

  • ISO 17025:2005 General requirements for the competence of testing and calibration laboratories
  • EN 16362:2014 Trichothecenes in food - Sampling and sample preparation
  • EN 16824:2016 Trichothecenes in food - Determination by liquid chromatography-tandem mass spectrometry (LC-MS/MS)
  • TSE (Turkish Standards Institution) - Equivalent to EU standards for trichothecene testing
  • Legal and Regulatory Framework:

    EN 15891 is a harmonized European standard that is recognized by the European Union. The standard ensures that laboratories are competent in performing trichothecene testing, thereby ensuring product safety and compliance with regulatory requirements.

    International and National Standards:

    The International Organization for Standardization (ISO) has published ISO 17025:2005, which sets out general requirements for laboratory competence. This standard is adopted by many countries worldwide, including the European Union.

    In addition to EN 15891, other national standards are used for trichothecene testing in various countries:

  • United States: ASTM D6597-14 Standard Practice for Sampling and Sample Preparation of Trichothecenes in Foods
  • China: GB/T 20963.3-2018 Determination of Trichothecenes in Food by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
  • Standard Development Organizations:

    The European Committee for Standardization (CEN) and the International Organization for Standardization (ISO) are two prominent standard development organizations responsible for developing standards related to trichothecene testing.

    Evolution of Standards:

    Standards evolve over time as new technologies emerge, and regulatory requirements change. Eurolab ensures that its laboratory equipment and procedures comply with the latest standards and regulations.

    Standard Numbers and Scope:

    EN 15891:

  • Title: Determination of trichothecenes in food using liquid chromatography-tandem mass spectrometry (LC-MS/MS)
  • Number: EN 15891
  • Scope: The standard covers the requirements for determining trichothecenes in food using LC-MS/MS laboratory testing.
  • Standard Compliance Requirements:

    Companies must comply with relevant standards, including EN 15891, to ensure product safety and regulatory compliance. Eurolabs laboratory is accredited to ISO 17025:2005, ensuring that our trichothecene testing services meet the required standards.

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    The need for EN 15891 Determination of Trichothecenes in Food Using LC-MS Testing:

  • Business Reasons: Companies must ensure product safety to maintain consumer trust and comply with regulatory requirements.
  • Technical Reasons: Trichothecene testing is essential for detecting mycotoxin contamination, which can have severe health consequences.
  • Consequences of Not Performing This Test:

    Failure to perform trichothecene testing can result in:

  • Consumer harm due to contaminated products
  • Loss of business and reputation
  • Regulatory non-compliance and fines
  • Industries and Sectors that Require This Testing:

    Food manufacturers, importers, exporters, and regulatory bodies require trichothecene testing services.

    Risk Factors and Safety Implications:

    Trichothecenes pose a significant risk to human health due to their potential toxicity. Regular monitoring of food products is essential to prevent contamination.

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    Step-by-Step Explanation of the Test:

    1. Sample preparation

    2. Instrument calibration

    3. LC-MS/MS analysis

    4. Data interpretation and reporting

    Testing Equipment and Instruments Used:

  • Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) instrument
  • Autosampler
  • Column oven
  • Tandem mass spectrometer
  • Testing Environment Requirements:

    Temperature: 20C 1C

    Humidity: 50 10

    Pressure: Atmospheric pressure

    Sample Preparation Procedures:

    Samples are extracted using a mixture of solvents and analyzed using LC-MS/MS.

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    Test Report Requirements:

    Eurolabs test report will include:

  • Sample identification
  • Method used
  • Results
  • Limitations and uncertainties
  • Certification of the analyst
  • Certification of the Analyst:

    Our analysts are certified to perform trichothecene testing according to EN 15891.

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    Eurolabs trichothecene testing services, based on EN 15891, ensure product safety and regulatory compliance. Our laboratory is accredited to ISO 17025:2005, guaranteeing the quality of our results. If you are a food manufacturer or importer/exporter, it is essential to work with a reputable laboratory like Eurolab for your trichothecene testing needs.

    References:

  • EN 15891
  • ISO 17025:2005
  • ASTM D6597-14
  • GB/T 20963.3-2018
  • Tables and Figures:

  • Table 1: Trichothecenes detected by LC-MS/MS
  • Figure 1: LC-MS/MS chromatogram of trichothecene standards
  • Need help or have a question?
    Contact us for prompt assistance and solutions.

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