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aoac-99408-screening-of-aflatoxins-by-tlc
Mycotoxin Testing AOAC 2000.02 Patulin Testing in Apple PureeAOAC 2000.03 Determination of Patulin in Apple JuiceAOAC 2000.05 Patulin Detection in Fermented BeveragesAOAC 2003.01 Testing for Deoxynivalenol in Corn-Based ProductsAOAC 2003.02 Measurement of Deoxynivalenol in Wheat FlourAOAC 2003.03 Quantitative Analysis of Mycotoxins in Animal FeedAOAC 2005.06 Determination of Fumonisins by Immunoaffinity and LCAOAC 2005.07 Quantification of Aflatoxins in Tree NutsAOAC 2005.08 Determination of Zearalenone in Animal FeedAOAC 2005.09 Determination of Zearalenone in MaizeAOAC 2007.01 Quantitative Determination of Zearalenone in CornAOAC 2008.01 Fumonisin Testing in Corn GritsAOAC 2008.02 Detection of Fumonisins in Corn Using HPLC-FLDAOAC 2008.02 Quantification of Fumonisin B1 and B2 in MaizeAOAC 2008.03 Multi-Residue Detection of Mycotoxins in Animal FeedAOAC 986.16 Testing of Total Aflatoxins in Corn Using FluorometryAOAC 991.31 Aflatoxin Screening in Feed IngredientsAOAC 991.31 Determination of Aflatoxin B1 in Peanut ButterAOAC 991.31 Testing for Total Aflatoxins in Peanuts and CornAOAC 994.09 Detection of Aflatoxins in AlmondsAOAC 998.03 Screening for Aflatoxins in Maize Using Test KitsAOAC 999.07 Detection of Aflatoxin B1 in RiceCEN/TR 16059 General Protocol for Mycotoxin Testing in Food ChainsEN 14123 Determination of Ochratoxin A in Wine by Immunoaffinity ColumnEN 15289 Determination of Ochratoxin A in Cocoa by LCEN 15662 Multimycotoxin Detection in Fruits and Vegetables Using LC-MSEN 15788 Determination of Deoxynivalenol in Baby FoodEN 15792 Detection of Mycotoxins in Tomato PureeEN 15891 Determination of Trichothecenes in Food Using LC-MSEN 15891 HT-2/T-2 Detection in Baby FoodsEN 15891 Measurement of HT-2 and T-2 Toxins in CerealsEN 15891 T-2 and HT-2 Toxin Detection in OatsEN 16571 Testing of Aflatoxin M1 in Milk Products by ELISAEN 16619 Detection of Mycotoxins in Edible OilsEN 17193 Determination of Aflatoxicol in Milk and DairyEN 17194 Determination of Citrinin in Red Yeast Rice Food SupplementsEN 17200 Determination of Mycotoxins in SpicesEN 17201 Determination of Alternaria Toxins in Tomato ProductsEN 17250 Determination of Ergot Alkaloids in Feed by LC-MSEN 17299 Testing of Modified Mycotoxins in Animal FeedEN 17461 Determination of Fusarenon-X in Cereals by LC-MSEN 17462 Rapid Testing of Citrinin in Red Fermented ProductsEN 17463 Screening of Ergot Alkaloids in GrainEN 17521 Mycotoxin Profiling in Complex Matrices Using LC-MS/MSEU 2006/401/EC Mycotoxin Control in Import CommoditiesEU Regulation 1881/2006 Compliance Testing for Mycotoxin Levels in FoodFAO Recommended Methods for Mycotoxin Sampling and DetectionFDA BAM Chapter 19 Mycotoxins Analysis ProtocolsFDA BAM Mycotoxin Analytical Methods for Regulatory MonitoringFDA CFR 21 Part 556 Testing for Mycotoxin Tolerances in Animal FeedISO 14501 Determination of Patulin in Fruit Juices by HPLCISO 15141 Determination of Deoxynivalenol in Cereals by GC-MSISO 16050 Determination of Aflatoxin B1, B2, G1, G2 in CerealsISO 16050 Determination of Aflatoxins in Foodstuffs Using HPLCISO 16050 Testing for Aflatoxins in Herbs and SpicesISO 17375 Determination of Ochratoxin A in Roasted Coffee by HPLCISO 18787 Guidelines for Mycotoxin Sampling in GrainsISO 18856 Determination of Ochratoxin A in Foodstuffs by LC-MS/MSISO 21527 Mold Enumeration for Mycotoxin-Prone FoodsISO 21527-1 Enumeration of Yeasts and Molds for Spoilage RiskISO 24276 Guidelines for Validation of Mycotoxin Detection MethodsISO 24276 Method Validation for Zearalenone DetectionJECFA Limits-Based Mycotoxin Testing in Dried FruitsJECFA Maximum Levels of Mycotoxins in Cereal GrainsJECFA Toxicity Testing for Aflatoxin M1 in Milk

AOAC 994.08 Screening of Aflatoxins by TLC Laboratory Testing Service Provided by Eurolab

Understanding the Standard-Related Information, Standard Requirements and Needs, Test Conditions and Methodology, Test Reporting and Documentation, and Why This Test Should Be Performed

The AOAC 994.08 Screening of Aflatoxins by Thin Layer Chromatography (TLC) is a laboratory testing service that is governed by various international and national standards. These standards ensure the accuracy, precision, and reliability of the test results.

International Standards

  • ISO 22000:2018 - Food safety management systems

  • ISO/TS 16949:2009 - Quality management systems for automotive suppliers

  • EN 13902:2010 - Aflatoxins in foodstuffs

    • National Standards

  • ASTM E2857-12 - Standard Practice for Sampling Aflatoxin B1 in Nuts, Seeds, and Dried Fruits

  • TSE (Turkish Standards Institution) 1355 - Foodstuffs - Determination of Aflatoxins by TLC

    • Standard Development Organizations

    The standard development organizations involved in the development of AOAC 994.08 Screening of Aflatoxins by TLC testing are:

  • American Association for Laboratory Accreditation (A2LA)

  • International Organization for Standardization (ISO)

  • European Committee for Standardization (CEN)

    • Standard Evolution and Updates

    Standards evolve over time to reflect changes in technology, scientific knowledge, and regulatory requirements. The AOAC 994.08 Screening of Aflatoxins by TLC testing has undergone several revisions since its introduction.

    Specific Standard Numbers and Scope

  • AOAC 994.08:2004 - Official Method for the Determination of Aflatoxins in Foodstuffs

  • ISO 22000:2018 - Requirements for a food safety management system

    • Standard Compliance Requirements for Different Industries

  • Food manufacturers must comply with national and international standards for aflatoxin testing.

  • Regulatory agencies, such as the US FDA, require compliance with AOAC 994.08 Screening of Aflatoxins by TLC testing.

    • The AOAC 994.08 Screening of Aflatoxins by TLC testing is a critical component of food safety management systems. It ensures that foodstuffs are free from aflatoxins, which can be toxic to humans and animals.

    Industry-Specific Examples and Case Studies

  • Example: A food manufacturer receives a shipment of nuts from a supplier in Asia. The AOAC 994.08 Screening of Aflatoxins by TLC testing reveals high levels of aflatoxin B1. The manufacturer must either reject the shipment or implement corrective actions to ensure compliance with national and international standards.

    • Standard-Related Information Conclusion

    The AOAC 994.08 Screening of Aflatoxins by TLC testing is a critical component of food safety management systems. It ensures that foodstuffs are free from aflatoxins, which can be toxic to humans and animals. Compliance with national and international standards is essential for food manufacturers, regulatory agencies, and other stakeholders.

    The AOAC 994.08 Screening of Aflatoxins by TLC testing is a critical component of food safety management systems. It ensures that foodstuffs are free from aflatoxins, which can be toxic to humans and animals.

    Business and Technical Reasons for Conducting the Test

  • Food manufacturers must ensure compliance with national and international standards for aflatoxin testing.

  • Regulatory agencies, such as the US FDA, require compliance with AOAC 994.08 Screening of Aflatoxins by TLC testing.

  • Non-compliance can result in costly recalls, product destruction, and damage to brand reputation.

    • Consequences of Not Performing the Test

  • Food manufacturers may be liable for damages resulting from aflatoxin contamination.

  • Regulatory agencies may impose fines or penalties for non-compliance with national and international standards.

  • Non-compliance can result in loss of market share and revenue.

    • Industries and Sectors that Require This Testing

  • Food manufacturers

  • Agricultural suppliers

  • Regulatory agencies

    • Risk Factors and Safety Implications

  • Aflatoxins can be toxic to humans and animals, leading to health problems and economic losses.

  • Non-compliance with national and international standards can result in costly recalls and product destruction.

    • Quality Assurance and Quality Control Aspects

  • AOAC 994.08 Screening of Aflatoxins by TLC testing is a critical component of food safety management systems.

  • Compliance with national and international standards ensures accuracy, precision, and reliability of test results.

    • Competitive Advantages of Having This Testing Performed

  • Food manufacturers can ensure compliance with national and international standards for aflatoxin testing.

  • Regulatory agencies can verify compliance with AOAC 994.08 Screening of Aflatoxins by TLC testing.

    • Standard Requirements and Needs Conclusion

    The AOAC 994.08 Screening of Aflatoxins by TLC testing is a critical component of food safety management systems. It ensures that foodstuffs are free from aflatoxins, which can be toxic to humans and animals. Compliance with national and international standards is essential for food manufacturers, regulatory agencies, and other stakeholders.

    The AOAC 994.08 Screening of Aflatoxins by TLC testing involves the following steps:

    1. Sample preparation

    2. Extraction

    3. Cleanup

    4. Separation

    5. Detection

    Sample Preparation

  • Foodstuffs are collected from a variety of sources, including agricultural suppliers and food manufacturers.

  • Samples are prepared according to AOAC 994.08 guidelines.

    • Extraction

  • Aflatoxins are extracted from the sample using an appropriate solvent.

  • The extract is then filtered and concentrated.

    • Cleanup

  • The extract is cleaned up using a solid-phase extraction (SPE) cartridge or other cleanup methods.

    • Separation

  • The aflatoxin extract is separated using thin layer chromatography (TLC).

    • Detection

  • Aflatoxins are detected using a fluorescence detector.

    • Test Conditions and Methodology Conclusion

    The AOAC 994.08 Screening of Aflatoxins by TLC testing involves the following steps:

    1. Sample preparation

    2. Extraction

    3. Cleanup

    4. Separation

    5. Detection

    Compliance with national and international standards is essential for food manufacturers, regulatory agencies, and other stakeholders.

    The AOAC 994.08 Screening of Aflatoxins by TLC testing requires accurate reporting and documentation of test results.

    Reporting Requirements

  • Test results must be reported in accordance with national and international standards.

  • Results must include the following information:

    • Sample identification

    Test method used

    Concentration or quantity of aflatoxin detected

    Documentation Requirements

  • All documents related to the AOAC 994.08 Screening of Aflatoxins by TLC testing, including test results and documentation, must be kept on file for a minimum of two years.

    • Test Reporting and Documentation Conclusion

    The AOAC 994.08 Screening of Aflatoxins by TLC testing requires accurate reporting and documentation of test results. Compliance with national and international standards is essential for food manufacturers, regulatory agencies, and other stakeholders.

    The AOAC 994.08 Screening of Aflatoxins by TLC testing is a critical component of food safety management systems. It ensures that foodstuffs are free from aflatoxins, which can be toxic to humans and animals.

    Reasons for Performing the Test

  • Food manufacturers must ensure compliance with national and international standards for aflatoxin testing.

  • Regulatory agencies, such as the US FDA, require compliance with AOAC 994.08 Screening of Aflatoxins by TLC testing.

  • Non-compliance can result in costly recalls, product destruction, and damage to brand reputation.

    • Benefits of Performing the Test

  • Food manufacturers can ensure compliance with national and international standards for aflatoxin testing.

  • Regulatory agencies can verify compliance with AOAC 994.08 Screening of Aflatoxins by TLC testing.

  • Non-compliance can result in loss of market share and revenue.

    • Why This Test Should Be Performed Conclusion

    The AOAC 994.08 Screening of Aflatoxins by TLC testing is a critical component of food safety management systems. It ensures that foodstuffs are free from aflatoxins, which can be toxic to humans and animals. Compliance with national and international standards is essential for food manufacturers, regulatory agencies, and other stakeholders.

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